A5054959768- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Phytoplasmas and Hemiptera pathogens
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Insect Resistance and Genetics
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Cocoa and Sweet Potato Agronomy
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- Pineapple and bromelain studies
- Banana Cultivation and Research
- Agricultural pest management studies
- Insect symbiosis and bacterial influences
- Legume Nitrogen Fixing Symbiosis
- Viral Infections and Vectors
- Pacific and Southeast Asian Studies
- Plant pathogens and resistance mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Plant Disease Management Techniques
University of Hawaii System
2015-2024
University of Hawaiʻi at Mānoa
2010-2023
Chinese Academy of Tropical Agricultural Sciences
2023
Ministry of Agriculture and Rural Affairs
2023
Hebei Agricultural University
2017
Chinese Academy of Agricultural Sciences
2017
Guangdong Academy of Agricultural Sciences
2017
University of Rochester
2017
Institute of Plant Protection
2017
William & Mary
2010
Proteins containing the basic-helix-loop-helix (B-HLH) domain have been shown to be important in regulating cellular differentiation. We isolated a cDNA for human B-HLH factor, denoted HEB, that shares nearly complete identity with immunoglobulin enhancer binding proteins encoded by E2A and ITF2 genes (E proteins). Functional characterization of protein expressed from this indicates HEB is third member E-protein class factors. mRNA was found several tissues cell types, including skeletal...
Abstract Maintenance of indeterminacy is fundamental to the generation plant architecture and a central component life strategy. Indeterminacy in plants characteristic shoot root meristems, which must balance maintenance with organogenesis. The Petunia hybrida HAIRY MERISTEM (HAM) gene, member GRAS family transcriptional regulators, promotes by an undefined non-cell-autonomous signaling mechanism(s). Here, we report that Arabidopsis (Arabidopsis thaliana) mutants triply homozygous for...
The literature on global trends in recreational fishing, the determinants of participation and social embedding fishing public eye are reviewed across world. Data support a conceptual life-cycle model fisheries according to which interest rises rapidly with economic development before leveling off eventually declining. Participation angling globe varies substantially is directly related societal-level developments affecting resources, time, socialization into fishing. Moreover, culture way...
Summary Banana bunchy top virus (BBTV) is a ssDNA transmitted by the banana aphid, ( Pentalonia nigronervosa ). A polymerase chain reaction (PCR) assay was used to study BBTV transmission efficiency, determine minimum acquisition‐access period, inoculation‐access retention time, and examine possibility of transovarial in this vector. acquired aphids within 4 h 15 min feeding. On average, more than 65% single viruliferous adult BBTV. The retained for their adulthood 15–20 days. None 131...
A Citrus volkameriana tree displaying symptoms similar to citrus leprosis on its leaves and bark was found in Hawaii. virus C (CiLV-C)-specific detection assays, however, were negative for all tissues tested. Short, bacilliform virus-like particles observed by transmission electron microscopy the cytoplasm of symptomatic but not healthy controls. Double-stranded (ds) RNAs ≈8 3 kbp size present leaf tissue Excluding poly(A) tails, largest molecule, RNA1, 8,354 bp length. The ≈3 dsRNA band be...
ABSTRACT Closterovirus-like particles associated with mealybug wilt of pineapple were acquired and transmitted by the pink mealybug, Dysmicoccus brevipes, gray D. neobrevipes. Mealybugs wilt-associated virus (PMWaV) from infected plants or detached leaves. The was detected in tissue blot immunoassay confirmed immunosorbent electron microscopy. Plants exposed to mealybugs reared on PMWaV-free remained uninfected. presence ants correlated an increased rate spread when caged brevipes. All...
The coat protein genes of two cucumber mosaic virus (CMV) isolates infecting banana plants in Hawaii were cloned and sequenced. Based on nucleotide amino acid sequence comparisons, both belong to CMV subgroup I. One isolate (CMV-Hawaii), which is common the state induces mild symptoms, shares 99% identity (both sequences) with CMV-C strain. Another (CMV-Oahu), was found only at farms Hawaii, severe leaf distortion symptoms. CMV-Oahu 91% 93% identity, respectively, CMV-Hawaii. A reverse...
Abstract A purification procedure (Z ee et al. 1987) for closterovirus‐like particles associated with grapevine leafroll disease was improved after various sources of plant tissues, extraction buffers, pH values, and centrifugation techniques had been examined. An antiserum against the CA‐4 isolate produced used in ELISA. The virus‐like NY‐1 measured crude preparations were about 1,800 to 1,900 nm long. molecular weight coat protein NY‐1and NY‐2 isolates ca. 43 × 10 3 daltons SDS‐PAGE...
Tomato mottle mosaic virus (ToMMV) is a recently identified species in the genus Tobamovirus and was first reported from greenhouse tomato sample collected Mexico 2013. In August 2013, ToMMV detected on peppers (Capsicum spp.) China. However, little known about molecular biological characteristics of ToMMV.
Dickeya zeae , a bacterial plant pathogen of the family Pectobacteriaceae, is responsible for wide range diseases on potato, maize, rice, banana, pineapple, taro, and ornamentals significantly reduces crop production. D. causes soft rot taro ( Colocasia esculenta ) heart pineapple Ananas comosus ). In this study, we used Pacific Biosciences single-molecule real-time (SMRT) sequencing to sequence two high-quality complete genomes novel strains : PL65 (size: 4.74997 MB; depth: 701x; GC: 53.6%)...
Approximately 3,600 orchid plants representing 44 genera from three collections, 22 commercial farms, and six nurseries on the islands of Oahu Hawaii were tested for Cymbidium mosaic virus (CyMV), Odontoglossum ringspot (ORSV), tomato spotted wilt (TSWV), potyviruses with enzyme-linked immunosorbent assay (ELISA). CyMV was detected in 59 samples ORSV 23 samples, which represented 61 25% surveyed, respectively. Double infection both viruses occurred 20 20% genera. 29 seven 31 sites respectively
Mealybug wilt of pineapple (MWP) is one the most destructive diseases (Ananas comosus) worldwide. At least Ampelovirus species, Pineapple mealybug associated virus-2 (PMWaV-2), and feeding are involved in etiology MWP. A previously undescribed sharing highest homology with PMWaV-1 a putative deletion mutant PMWaV-2 were detected reverse transcription-polymerase chain reaction (RT-PCR) assays using degenerate primers. Results verified additional sequence information by immunosorbent electron...
Bacterial soft rot of banana was first noticed in 2009 Guangzhou city, China. The disease also observed on various cultivars different genotypes several other cities. Symptoms the included leaf wilting, collapse pseudostems, and unusual odor. Five isolated strains that fulfilled Koch's postulates were used for biochemical testing. five most similar to Dickeya dadantii or D. zeae, but much less paradisiaca when using phenotype characteristics. Sequence analysis 16S rDNA, dnaX, gryB, recA a...
Ti ringspot is an emerging foliar disease of the ti plant (Cordyline fruticosa) in Hawaii that quickly spreading throughout islands. Symptoms include small chlorotic ringspots on leaves often coalesce to form larger lesions. Although several virus species have been discovered symptomatic plants, none associated with these symptoms. Here, we report and characterize a novel closely symptoms Hawaii. The presence double membrane bodies approximately 85 nm diameter cells sequence analyses five...
Hibiscus ( spp.) are popular ornamental and landscape plants in Hawaii which susceptible to foliar diseases caused by viruses belonging the genera Cilevirus Higrevirus (family Kitaviridae ). In this study, a virus infecting H. rosa-sinensis displaying symptoms consistent with infection kitavirus, including yellow chlorotic blotches green perimeter, was characterized. The genome consisted of two RNAs 8.4 4.4 kb length, organized most similarly cileviruses, but important distinctions. These...
The roles of Pineapple mealybug wilt-associated viruses (PMWaVs) and (Dysmicoccus spp.) feeding in the etiology wilt pineapple (MWP) were evaluated. Container-grown (Ananas comosus) plants from five commercially grown Hawaiian proprietary selections a field study utilizing randomized complete block design used to test four treatments for induction MWP: PMWaV-1-free PMWaV-1-infected maintained mealybug-free, that received monthly applications nonviruliferous mealybugs. A second PMWaV,...
A number of cucurbit-infecting tobamoviruses have been reported in the past but there is confusion about their identity and relationships. Cucumber viruses 3 (CV3) 4 (CV4) were originally described United Kingdom whereas watermelon (W) cucumber (C) 'isolates' green mottle mosaic virus (CGMMV) came from Japan. The results serological studies RNA-cDNA analyses shown that CV3, CV4 CGMMV-W are very closely related, CGMMV-C quite different. It concluded two distinct infect cucurbits; they only...