A5012549742- Zebrafish Biomedical Research Applications
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Spinal Cord Injury Research
- Developmental Biology and Gene Regulation
- Advanced Fluorescence Microscopy Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Photoreceptor and optogenetics research
- Cancer-related gene regulation
- Electrospun Nanofibers in Biomedical Applications
- Polymer Surface Interaction Studies
- Ocular and Laser Science Research
- Wnt/β-catenin signaling in development and cancer
- Cellular Mechanics and Interactions
- Signaling Pathways in Disease
- Proteoglycans and glycosaminoglycans research
- Planarian Biology and Electrostimulation
- MicroRNA in disease regulation
- Optical Coherence Tomography Applications
- Connective tissue disorders research
- Orthopaedic implants and arthroplasty
- Congenital heart defects research
- Traumatic Brain Injury and Neurovascular Disturbances
- Spectroscopy Techniques in Biomedical and Chemical Research
Technische Universität Dresden
2011-2024
Max Planck Institute for the Science of Light
2020-2024
ETH Zurich
2024
University of Edinburgh
2016-2021
Discovery Centre
2019-2020
Memorial Medical Center
2016
Universität Ulm
2013-2015
Abertay University
2011
Wright State University
1984
Abstract Spinal cord injury leads to a massive response of innate immune cells in non-regenerating mammals, but also successfully regenerating zebrafish. However, the role successful regeneration is poorly defined. Here we show that inhibiting inflammation reduces and promoting it accelerates axonal spinal-lesioned zebrafish larvae. Mutant analyses peripheral macrophages, not neutrophils or microglia, are necessary for repair. Macrophage-less irf8 mutants prolonged with elevated levels Tnf-α...
Zebrafish regenerate their fins via the formation of a population progenitor cells, blastema. Wnt/β-catenin signaling is essential for blastemal cell proliferation and patterning overlying epidermis. Yet, we find that β-catenin neither active in epidermis nor majority proliferative cells. Rather, tissue-specific pathway interference indicates Wnt nonproliferative distal blastema required proximal blastema, cells lining osteoblasts directs osteoblast differentiation. Thus, regulates epidermal...
Abstract The inhibitory extracellular matrix in a spinal lesion site is major impediment to axonal regeneration mammals. In contrast, the zebrafish allows substantial axon re-growth, leading recovery of movement. However, little known about regulation and composition growth-promoting matrix. Here we demonstrate that activity Wnt/β-catenin pathway fibroblast-like cells pivotal for re-growth functional recovery. signaling induces expression col12a1a/b deposition Collagen XII, which necessary...
In adult zebrafish, relatively quiescent progenitor cells show lesion-induced generation of motor neurons. Developmental neuron from the spinal domain (pMN) sharply declines already at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated same domain. We demonstrate here that within after a lesion or specific genetic ablation neurons 72 hpf, pMN reverts to expense oligodendrogenesis. contrast, dorsal Pax2-positive interneurons was not altered. Larval...
Zebrafish have an unlimited capacity to regenerate bone after fin amputation. In this process, mature osteoblasts dedifferentiate osteogenic precursor cells and thus represent important source of newly forming bone. By contrast, differentiated do not appear contribute repair injuries in mammals; rather, form anew from mesenchymal stem cells. This raises the question whether osteoblast dedifferentiation is specific appendage regeneration, a special feature lepidotrichia fish fin, or process...
Zebrafish can completely regenerate amputated fins via formation of a blastema, proliferative mass undifferentiated precursor cells. During regenerative growth, blastema proliferation must be tightly coordinated with cellular differentiation, but little is known about how this achieved. Here, we show that Notch signaling essential for maintenance cells in state. We found the pathway activated response to fin amputation highly region blastema. Chemical interference resulted complete block...
Wnt/β-catenin signaling regulates tissue homeostasis and regeneration in metazoans. In planarians-flatworms with high regenerative potential-Wnt ligands are thought to control polarity by shaping a β-catenin activity gradient along the anterior-posterior axis, yet downstream mechanisms poorly understood. We performed an RNA sequencing (RNA-seq)-based screen identified hundreds of β-catenin-dependent transcripts, which several were expressed muscle stem cells graded fashion. particular,...
Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in humans and other mammals, whereas it facilitates axon regeneration the zebrafish. However, molecular basis of these different fates is not understood. Here, we identify small leucine-rich proteoglycans (SLRPs) as a contributing factor failure mammals. We demonstrate that SLRPs chondroadherin, fibromodulin, lumican, prolargin are enriched rodent human but zebrafish CNS lesions....
After peripheral nerve injury, mature Schwann cells (SCs) de-differentiate and undergo cell reprogramming to convert into a specialized repair phenotype that promotes regeneration. Reprogramming of SCs the is tightly controlled at genome level includes downregulation pro-myelinating genes activation repair-associated genes. Nerve injuries induce not only biochemical but also mechanical changes in tissue architecture which impact SCs. Recently, we showed mechanically sense stiffness...
Fibrosis and persistent inflammation are interconnected processes that inhibit axon regeneration in the mammalian central nervous system (CNS). In zebrafish, by contrast, fibroblast-derived extracellular matrix deposition tightly regulated to facilitate regeneration. However, regulatory cross-talk between fibroblasts innate immune regenerating CNS remains poorly understood. Here, we show zebrafish possess a dual role inducing resolving inflammation, which both essential for We identify...
The design and synthesis of a polyphenylene dendrimer ( PPD 3 ) with discrete binding sites for lipophilic guest molecules characteristic surface patterns is presented. Its semi‐rigidity in combination precise positioning hydrophilic hydrophobic groups at the periphery yields refined architecture pockets that accommodate defined numbers biologically relevant such as fatty acids or drug doxorubicin. size, architecture, textures allow to even penetrate brain endothelial cells are major...
The quantification of physical properties biological matter gives rise to novel ways understanding functional mechanisms. One the basic biophysical is mass density (MD). It affects dynamics in sub-cellular compartments and plays a major role defining opto-acoustical cells tissues. As such, MD can be connected refractive index (RI) via well known Lorentz-Lorenz relation, which takes into account polarizability matter. However, computing based on RI measurements poses challenge, as it requires...
Spinal cord injury (SCI) results in loss of neurons, oligodendrocytes and myelin sheaths, all which are not efficiently restored. The scarcity the lesion site impairs re-myelination spared fibres, leaves axons denuded, impedes signal transduction contributes to permanent functional deficits. In contrast mammals, zebrafish can functionally regenerate spinal cord. Yet, little is known about oligodendroglial lineage biology capacity after SCI a regeneration-permissive context. Here, we report...
Abstract Understanding the photodamage mechanism underlying highly nonlinear dynamic of femtosecond laser pulses at second transparent window tissue is crucial for label-free microscopy. Here, we report identification two cavitation regimes from 1030 nm when interacting with central nervous system in zebrafish. We show that low repetition rates, damage confined due to plasma-based ablation and sudden local temperature rise. At high becomes collateral plasma-mediated photochemistry....
Chondrolectin (Chodl) is needed for motor axon extension in zebrafish and dysregulated mouse models of spinal muscular atrophy (SMA). However, the mechanistic basis Chodl function not known. Here, we use Chodl-deficient mutants to show that absence leads anatomical functional defects neuromuscular synapse. In zebrafish, growth an identified beyond "en passant" synapse later branching from synaptic points are impaired, leading deficits. Mechanistically, motor-neuron-autonomous depends on its...
Zebrafish regenerate their spinal cord after injury, both at larval and adult stages. Larval zebrafish have emerged as a powerful model system to study injury regeneration due high optical transparency for in vivo imaging, amenability high-throughput analysis, rapid time. Here, we describe protocol the mechanical transection of cord, followed by whole-mount tissue processing situ hybridization immunohistochemistry elucidate principles regeneration. For complete details on use execution this...