A5036067145- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Proteoglycans and glycosaminoglycans research
- Plant-Microbe Interactions and Immunity
- Light effects on plants
- Oral and Maxillofacial Pathology
- Plant biochemistry and biosynthesis
- Plant Parasitism and Resistance
- Polysaccharides and Plant Cell Walls
- Bone and Dental Protein Studies
- Plant and animal studies
- Plant Virus Research Studies
- Glycosylation and Glycoproteins Research
- Plant Genetic and Mutation Studies
- Postharvest Quality and Shelf Life Management
- RNA and protein synthesis mechanisms
- Plant responses to water stress
- Seed Germination and Physiology
- Plant Micronutrient Interactions and Effects
- Cell Adhesion Molecules Research
National Cheng Kung University
2022-2024
National University of Tainan
2024
Radboud University Nijmegen
2024
Saitama University
2014-2023
National Institute of Advanced Industrial Science and Technology
2013-2022
Suntory (Japan)
2014
Japan Science and Technology Agency
2003-2008
Nihon University
1984-2008
Kanazawa University
2008
Tokyo Medical and Dental University
1984-2006
We demonstrated that the GCC box, which is an 11-bp sequence (TAAGAGCCGCC) conserved in 5' upstream region of ethylene-inducible pathogenesis-related protein genes Nicotiana spp and some other plants, essential for ethylene responsiveness when incorporated into a heterologous promoter. Competitive gel retardation assays showed DNA binding activities to be specific box tobacco nuclear extracts. Four different cDNAs encoding proteins were isolated, their products designated ethylene-responsive...
Ethylene-responsive element binding factors (ERFs) are members of a novel family transcription that specific to plants. A highly conserved DNA domain known as the ERF is unique feature this protein family. To characterize in detail factors, we isolated Arabidopsis cDNAs encoding five different proteins (AtERF1 AtERF5) and analyzed their structure, preference, transactivation ability, mRNA expression profiles. The AtERFs were placed into three classes based on amino acid identity within...
ABSCISIC ACID-RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) (i.e., ABF2) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element (ABRE) motif in promoter region of ABA-inducible genes. Here, we show expression intact AREB1 gene on its own insufficient lead downstream genes under normal growth conditions. To overcome masked transactivation activity AREB1, created an activated form (AREB1DeltaQT). AREB1DeltaQT-overexpressing plants showed...
Summary Arabidopsis thaliana RD26 cDNA, isolated from dehydrated plants, encodes a NAC protein. Expression of the gene was induced not only by drought but also abscisic acid (ABA) and high salinity. The protein is localized in nucleus its C terminal has transcriptional activity. Transgenic plants overexpressing were highly sensitive to ABA, while ‐repressed insensitive. results microarray analysis showed that ABA‐ stress‐inducible genes are upregulated ‐overexpressed repressed plants....
We reported previously that three ERF transcription factors, tobacco ERF3 (NtERF3) and Arabidopsis AtERF3 AtERF4, which are categorized as class II ERFs, active repressors of transcription. To clarify the roles these in transcriptional regulation plants, we attempted to identify functional domains repressor mediates repression Analysis results a series deletions revealed C-terminal 35 amino acids NtERF3 sufficient confer capacity for on heterologous DNA binding domain. This domain suppressed...
The redundancy of genes for plant transcription factors often interferes with efforts to identify the biologic functions such factors. We show here that four different fused EAR motif, a repression domain only 12 amino acids, act as dominant repressors in transgenic Arabidopsis and suppress expression specific target genes, even presence redundant factors, resultant loss-of-function phenotypes. Chimeric EIN3, CUC1, PAP1, AtMYB23 included motif dominantly suppressed their caused insensitivity...
Abstract Wood is formed by the successive addition of secondary xylem, which consists cells with a conspicuously thickened wall composed mainly lignin and cellulose. Several genes involved in cellulose biosynthesis have been characterized, but factors that regulate formation walls woody tissues remain to be identified. In this study, we show plant-specific transcription factors, designated NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1) NST3, are key regulators Arabidopsis thaliana....
Abstract In plants, secondary wall thickenings play important roles in various biological processes, although the factors regulating these processes remain to be characterized. We show that expression of chimeric repressors derived from NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1) and NST2 Arabidopsis thaliana resulted an anther dehiscence defect due loss thickening endothecium. Plants with double, but not single, T-DNA–tagged lines for NST1 had same anther-indehiscent phenotype as...
In Arabidopsis, MYB transcription factors regulate flavonoid biosynthesis via the formation of protein complexes with a basic helix-loop-helix (bHLH) factor and WD40 repeat protein. Several R3-type single-MYB proteins (R3-MYB), such as CPC TRY, act negative regulators development epidermal cells. However, have not yet been reported, to our knowledge. We show here that an R3-MYB protein, AtMYBL2, acts transcriptional repressor negatively regulates anthocyanin in Arabidopsis. AtMYBL2 knockout...
Ethylene-responsive element-binding proteins (EREBPs)have novel DNA-binding domains (ERF domains), which are widely conserved in plants, and interact specifically with sequences containing AGCCGCC motifs (GCC box). Deletion experiments show that some flanking region at the N terminus of 59-amino acid ERF domain is required for stable binding to GCC box. Three domain-containing fragments EREBP2, EREBP4, AtERF1 from tobacco Arabidopsis, bind sequence box a high affinity pm range. The conferred...
Plants form shoot meristems in the so-called boundary region, and these are necessary for normal morphogenesis of aerial parts plants. However, molecular mechanisms that regulate formation not fully understood. We report here expression a chimeric repressor from TCP3 (TCP3SRDX), member TEOSINTE BRANCHED1, CYCLOIDEA, PCF (TCP) transcription factors Arabidopsis thaliana, resulted ectopic shoots on cotyledons various defects organ development. Expression TCP3SRDX induced boundary-specific...
Abstract Coordination of the maintenance undifferentiated fate cells in shoot meristem and promotion cellular differentiation plant organs is essential for development shoots. CINCINNATA-like (CIN-like) TEOSINTE BRANCHED1, CYCLOIDEA, PCF (TCP) transcription factors are involved this coordination via negative regulation CUP-SHAPED COTYLEDON (CUC) genes, which regulate formation meristems specification organ boundaries. However, molecular mechanism action CIN-like TCPs poorly understood. We...
Constituting approximately 10% of flowering plant species, orchids (Orchidaceae) display unique flower morphologies, possess an extraordinary diversity in lifestyle, and have successfully colonized almost every habitat on Earth. Here we report the draft genome sequence Apostasia shenzhenica, a representative one two genera that form sister lineage to rest Orchidaceae, providing reference for inferring content structure most recent common ancestor all extant improving our understanding their...
Summary The Arabidopsis thaliana NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), acts as a key regulator of xylem vessel differentiation. In order to identify direct target genes VND7, we performed global transcriptome analysis using transgenic lines in which VND7 activity could be induced post‐translationally. This identified 63 putative encode broad range proteins, such factors, IRREGULAR XYLEM proteins and proteolytic enzymes, known closely associated with formation....
Abstract The Arabidopsis thaliana NAC domain transcription factor VASCULAR-RELATED NAC-DOMAIN7 (VND7) acts as a master regulator of xylem vessel differentiation. To understand the mechanism by which VND7 regulates differentiation, we used yeast two-hybrid system to screen for proteins that interact with and identified cDNAs encoding two proteins, VND-INTERACTING1 (VNI1) VNI2. Binding assays demonstrated VNI2 effectively interacts VND family VND1-5, well other at lower affinity. is expressed...
Abstract Many eukaryotes have from one to three heat shock factors (Hsfs), but plants more than 20 Hsfs, designated class A, B, and C. Class A Hsfs are activators of transcription, details the roles individual not been fully characterized. We show here that Arabidopsis (Arabidopsis thaliana) HsfB1 HsfB2b, members transcriptional repressors negatively regulate expression heat-inducible (HsfA2, HsfA7a, HsfB1, HsfB2b) several protein genes. In hsfb1 hsfb2b double mutant plants, a large number...
Abstract Most transcription factors act either as activators or repressors, and no such with dual function have been unequivocally identified characterized in plants. We demonstrate here that the Arabidopsis thaliana protein WUSCHEL (WUS), which regulates maintenance of stem cell populations shoot meristems, is a bifunctional factor acts mainly repressor but becomes an activator when involved regulation AGAMOUS (AG) gene. show WUS box, conserved among WOX genes, domain essential for all...
Orchids make up about 10% of all seed plant species, have great economical value, and are specific scientific interest because their renowned flowers ecological adaptations. Here, we report the first draft genome sequence a lithophytic orchid, Dendrobium catenatum. We predict 28,910 protein-coding genes, find evidence whole duplication shared with Phalaenopsis. observed expansion many resistance-related suggesting powerful immune system responsible for adaptation to wide range niches. also...
Abstract Jasmonates (JAs) are plant hormones that regulate the balance between growth and responses to biotic abiotic stresses. Although recent studies have uncovered mechanisms for JA-induced in Arabidopsis thaliana, by which plants attenuate remain elusive. Here, we report a basic helix-loop-helix–type transcription factor, ABA-INDUCIBLE BHLH-TYPE TRANSCRIPTION FACTOR/JA-ASSOCIATED MYC2-LIKE1 (JAM1), acts as transcriptional repressor negatively regulates JA signaling. Gain-of-function...
The waxy plant cuticle protects cells from dehydration, repels pathogen attack, and prevents organ fusion during development. transcription factor WAX INDUCER1/SHINE1 (WIN1/SHN1) regulates the biosynthesis of substances in Arabidopsis thaliana. Here, we show that MIXTA-like MYB factors MYB106 MYB16, which regulate epidermal cell morphology, also development coordinately with WIN1/SHN1 Torenia fournieri. Expression a chimeric repressor (35S:MYB106-SRDX) knockout/down MYB16 induced...
Significance Gibberellin (GA)-dependent degradation of DELLA protein, a key negative regulator, is essential for GA action. However, it unclear how regulates downstream gene expression. Although possessing strong transactivation activity, lacks DNA-binding domain. Therefore, model has been proposed in which acts as transcriptional coactivator with another transcription factor or factors containing Here, we show that some members the INDETERMINATE DOMAIN (IDD) protein family are such...