A5059176335- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant-derived Lignans Synthesis and Bioactivity
- Sesame and Sesamin Research
- Plant Stress Responses and Tolerance
- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
- Plant responses to water stress
- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- Peanut Plant Research Studies
- Phytoestrogen effects and research
- Plant Genetic and Mutation Studies
- Postharvest Quality and Shelf Life Management
- Ubiquitin and proteasome pathways
- Phytochemical compounds biological activities
- Banana Cultivation and Research
- Primate Behavior and Ecology
- Veterinary Medicine and Surgery
- Plant Growth Enhancement Techniques
Suntory Foundation for Life Sciences
2015-2024
Osaka Prefecture University
2022
University of Shiga Prefecture
2022
Suntory (Japan)
2022
Kyoto University
1996-2013
National Institute of Advanced Industrial Science and Technology
2003-2013
Japan Women's University
2010
Japan Science and Technology Agency
2003-2006
Iwate Biotechnology Research Center
2005
The University of Tokyo
2005
The redundancy of genes for plant transcription factors often interferes with efforts to identify the biologic functions such factors. We show here that four different fused EAR motif, a repression domain only 12 amino acids, act as dominant repressors in transgenic Arabidopsis and suppress expression specific target genes, even presence redundant factors, resultant loss-of-function phenotypes. Chimeric EIN3, CUC1, PAP1, AtMYB23 included motif dominantly suppressed their caused insensitivity...
Plants form shoot meristems in the so-called boundary region, and these are necessary for normal morphogenesis of aerial parts plants. However, molecular mechanisms that regulate formation not fully understood. We report here expression a chimeric repressor from TCP3 (TCP3SRDX), member TEOSINTE BRANCHED1, CYCLOIDEA, PCF (TCP) transcription factors Arabidopsis thaliana, resulted ectopic shoots on cotyledons various defects organ development. Expression TCP3SRDX induced boundary-specific...
Abstract Coordination of the maintenance undifferentiated fate cells in shoot meristem and promotion cellular differentiation plant organs is essential for development shoots. CINCINNATA-like (CIN-like) TEOSINTE BRANCHED1, CYCLOIDEA, PCF (TCP) transcription factors are involved this coordination via negative regulation CUP-SHAPED COTYLEDON (CUC) genes, which regulate formation meristems specification organ boundaries. However, molecular mechanism action CIN-like TCPs poorly understood. We...
The waxy plant cuticle protects cells from dehydration, repels pathogen attack, and prevents organ fusion during development. transcription factor WAX INDUCER1/SHINE1 (WIN1/SHN1) regulates the biosynthesis of substances in Arabidopsis thaliana. Here, we show that MIXTA-like MYB factors MYB106 MYB16, which regulate epidermal cell morphology, also development coordinately with WIN1/SHN1 Torenia fournieri. Expression a chimeric repressor (35S:MYB106-SRDX) knockout/down MYB16 induced...
Sophisticated regulation of gene expression, including microRNAs (miRNAs) and their target genes, is required for leaf differentiation, growth, senescence. The impact miR319 its TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR (TCP) genes on development has been extensively investigated, but the redundancies these families often interfere with evaluation function in developmental context. Here, we present genetic evidence involvement MIR319 TCP Arabidopsis...
Lateral organ traits in higher plants, such as lamina shape and trichome distribution, change gradually association with shoot maturation. Regulation of this maturation process the vegetative phase has been extensively investigated, members SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box family transcription factors have shown to be involved process. However, little is known about regulation reproductive phase. We analyzed SPL10, SPL11 SPL2, which are closely related SBP-box Arabidopsis. While...
Leaf senescence is the final process of leaf development that involves mobilization nutrients from old leaves to newly growing tissues. Despite identification several transcription factors involved in regulation this process, mechanisms underlying progression are largely unknown. Herein, we describe proteasome-mediated class II ETHYLENE RESPONSE FACTOR (ERF) transcriptional repressors and involvement these Arabidopsis (Arabidopsis thaliana). Based on previous results showing tobacco...
Photoperiod is one of the most reliable environmental cues for plants to regulate flowering timing. In Arabidopsis thaliana, CONSTANS (CO) transcription factor plays a central role in regulating photoperiodic flowering. contrast posttranslational regulation CO protein, still little was known about transcriptional regulation. Here we show that CINCINNATA (CIN) clade class II TEOSINTE BRANCHED 1/ CYCLOIDEA/ PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR (TCP) proteins act as activators. Our yeast...
Specific plant species produce unique isoquinoline alkaloids (IQAs); however, the mechanism of their evolution and regulation biosynthesis are largely unknown. We report here isolation a novel basic helix–loop–helix protein, CjbHLH1, from IQA-producing Coptis japonica. A BLAST search indicated that CjbHLH1 homologs were only found in IQAs. Transient RNA interference (RNAi) overexpression C. japonica protoplasts revealed activity transcription IQA biosynthetic genes, little genes involved...
Osmotin-like protein (OLP) is a neutral isoform in the group 5 pathogenesis-related (PR) tobacco proteins. The OLP gene, like basic PR genes, constitutively expressed roots and cultured cells. not naturally present intact healthy leaves, but ethylene treatment induces high accumulation there. To study mechanism of gene expression as induced by ethylene, we cloned from Nicotiana sylvestris, an ancestor N. tabacum. Sequence analysis showed that it has no intron its promoter region contains two...
A dehydration-inducible Arabidopsis CIN-like TCP gene, TCP13, acts as a key regulator of plant growth in leaves and roots under dehydration stress conditions. Plants modulate their shape response to environmental stress. However, regulatory mechanisms underlying the changes remain elusive. The CINCINNATA (CIN)-like TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) family transcription factors (TFs) are regulators for limiting through negative effect auxin response. Here, we report that stress-inducible...
Abstract Arabidopsis (Arabidopsis thaliana) ethylene-responsive element binding protein (AtEBP) gene was isolated as a suppressor of Bax-induced cell death by functional screening in yeast (Saccharomyces cerevisiae). To further examine the suppressive action AtEBP plant cells, we established transgenic tobacco (Nicotiana tabacum) plants overexpressing well ectopically expressing mouse Bax under dexamethasone-inducible promoter. We prepared crosses selective lines each plant, which were...
We clarified important roles of Arabidopsis TCP1, a member the TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) transcription factors, in longitudinal elongation petioles, rosette leaves inflorescent stems. found that promoter TCP1 was active cotyledonary petioles distal part expanding leaves, as well midrib region petiole.
Chimeric REpressor gene-Silencing Technology (CRES-T) is a powerful gene-silencing tool to analyze the function of Arabidopsis transcription factors. To investigate whether CRES-T also applicable horticultural plants inadequate for genetic engineering because their limited molecular biological characterization and polyploidy, we applied torenia hexaploid chrysanthemum produced transgenic expressing chimeric repressor derived from TEOSINTE BRANCHED1, CYCLOIDEA, PCF family factor 3 (TCP3)...
Plants grow under threats of environmental changes that could injure cellular viability and damage whole-plant physiology. To defend themselves against such threats, plants induce protective responses, including the production defense molecules. The red/purple pigment anthocyanin is synthesized upon leaf fruit development as well stimuli excess light exposure. Therefore, biosynthesis considered a model signaling pathway integration developmental responses. This tightly regulated by...
We reported previously that a chimeric repressor, in which transcription factor was fused to the EAR motif repression domain, acted as dominant repressor and suppressed expression of target genes, such resultant phenotypes were similar those associated with loss-of-function alleles. report here AtMYB23 induced variety morphological changes, namely ectopic formation root hairs, short primary root, elongation leaves inflorescence stems, absence accumulation mucilage on seed coats, addition...
The final shape of shoot lateral organs, namely, leaves and flowers, is determined by coordinated growth after the initiation primordia from meristems in seed plants. This coordination achieved complex action many transcription factors, which include TEOSINTE BRANCHED1, CYCLOIDEA, PCF (TCP) family. We have recently reported that CINCINNATA-like (CIN-like) TCP genes act dose-dependently to regulate flat smooth morphology Arabidopsis thaliana. In contrast, roles CIN-like flower development are...
Cyclamen persicum is among the most popular pot plants in world. Creating new ruffled flower petals an important breeding target this plant. TCP transcription factors are involved defining plant morphology angiosperms. To modify shape cyclamen, we isolated a gene from cyclamen (CpTCP1), which clustered same group as TCP3 (AtTCP3) and TCP4 (AtTCP4) Arabidopsis CINCINNATA (CIN) Antirrhinum majus. A chimeric repressor construct for CpTCP1 driven by cauliflower mosaic virus (CaMV) 35S promoter...
FioreDB (http://www.cres-t.org/fiore/public_db/) is a database of phenotypes induced by Chimeric REpressor gene-Silencing Technology (CRES-T) in ornamental and model plants. CRES-T induces loss-of-function phenotype transcription factor (TF) gene expression chimeric repressor produced fusion TF to the strong transcriptional repression domain (SRDX). The earlier version provided phenotypic information many kinds repressors various plants including torenia, chrysanthemum, cyclamen, gentian,...
Transcription factors of the basic Helix-Loop-Helix (bHLH) family play a central role in cell proliferation, determination, and differentiation. In plants, regulatory functions bHLHs phenylpropanoid biosynthesis have been well established with regard to other interacting-proteins; i.e., MYB WD40 repeat proteins. On hand, those alkaloid are greatly limited due distribution alkaloids plant species. Recently, several groups reported bHLH biosynthesis: novel CjbHLH1 isoquinoline Coptis japonica,...
Tobacco ETHYLENE-RESPONSIVE FACTOR3 (ERF3) is a member of the ERF-domain transcription factors and has transcriptional repressor activity, whereas other ERF proteins show activation activity. To understand regulation ERF3-repressor protein(s) were screened which interact with ERF3 in yeast two-hybrid system. A partial sequence (B8) NtUBC2, tobacco ubiquitin-conjugating enzyme was isolated. This B8 specifically interacted Further analyses revealed that region unique to B8. The physiological...