A5025184328- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- CRISPR and Genetic Engineering
- Plant Reproductive Biology
- Insect symbiosis and bacterial influences
- Bioenergy crop production and management
- Plant Gene Expression Analysis
- Insect behavior and control techniques
- Transgenic Plants and Applications
- Genetically Modified Organisms Research
- Spectroscopy and Chemometric Analyses
- Plant and animal studies
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Circadian rhythm and melatonin
- Chromosomal and Genetic Variations
Oregon State University
2021-2025
Zero to Three
2025
University of Washington
2017-2022
Photoperiod is one of the most reliable environmental cues for plants to regulate flowering timing. In Arabidopsis thaliana, CONSTANS (CO) transcription factor plays a central role in regulating photoperiodic flowering. contrast posttranslational regulation CO protein, still little was known about transcriptional regulation. Here we show that CINCINNATA (CIN) clade class II TEOSINTE BRANCHED 1/ CYCLOIDEA/ PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR (TCP) proteins act as activators. Our yeast...
CYCLING DOF FACTOR 1 (CDF1) and its homologs play an important role in the floral transition by repressing expression of activator genes such as CONSTANS (CO) FLOWERING LOCUS T (FT) Arabidopsis. The day-length-specific removal CDF1-dependent repression is a critical mechanism photoperiodic flowering. However, which CDF1 represses CO FT transcription remained elusive. Here we demonstrate that Arabidopsis CDF proteins contain non-EAR motif-like conserved domains required for interaction with...
The plant‐specific protein GIGANTEA ( GI ) controls many developmental and physiological processes, mediating rhythmic post‐translational regulation. physically binds several proteins implicated in the circadian clock, photoperiodic flowering, abiotic stress responses. To understand 's multifaceted function, we aimed to comprehensively quantitatively identify potential interactors of a time‐specific manner, using proteomics on Arabidopsis plants expressing epitope‐tagged . We detected...
Abstract Because of the limitations inherent in conventional breeding trees and clonally propagated crops, gene editing is great interest. Dozens published papers attest to high efficiency CRISPR-based systems clonal crops trees. The opportunity for “clean” edits expected avoid or reduce regulatory burdens many countries may improve market acceptance. To date, however, nearly all studies retained machinery genome. Despite efficiency, technical obstacles are likely greatly limit progress...
Wide variation in amenability to transformation and regeneration (TR) among many plant species genotypes presents a challenge the use of genetic engineering research breeding. To help understand causes this variation, we performed association mapping network analysis using population 1204 wild trees Populus trichocarpa (black cottonwood). enable precise high-throughput phenotyping callus shoot TR, developed computer vision system that cross-referenced complementary red, green, blue (RGB)...
Abstract The spread of transgenes and exotic germplasm from planted crops into wild or feral species is a difficult problem for public regulatory acceptance genetically engineered plants, particularly wind-pollinated trees such as poplar. We report that overexpression poplar homolog the floral repressor SHORT VEGETATIVE PHASE-LIKE (SVL) , Arabidopsis MADS-box PHASE (SVP) delayed onset flowering several years in three genotypes field-grown transgenic poplars. Higher expression SVL correlated...
Abstract Eucalyptus spp. are widely cultivated for the production of pulp, energy, essential oils, and as ornamentals. However, their dispersal from plantings, especially when grown an exotic, can cause ecological disruptions. To provide new tools prevention sexual by pollen well to induce male‐sterility hybrid breeding, we studied clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9‐mediated knockout three floral genes in both FT ‐expressing (early‐flowering) non‐...
Introduction: CRISPR gene editing, while highly efficient in creating desired mutations, also has the potential to cause off-target mutations. This risk is especially high clonally propagated plants, where editing reagents may remain genome for long periods of time or perpetuity. We studied a diverse population Populus and Eucalyptus trees that had CRISPR/Cas9-containing transgenes targeted one two types floral development genes, homologs LEAFY AGAMOUS . Methods: Using sequence approach, we...
The circadian clock represents a critical regulatory network, which allows plants to anticipate environmental changes as inputs and promote plant survival by regulating various physiological outputs. Here, we examine the function of clock-regulated transcription factor, CYCLING DOF FACTOR 6 (CDF6), during cold stress in Arabidopsis thaliana. We found that gates CDF6 transcript accumulation vasculature stress. mis-expression results an altered flowering phenotype both ambient A genome-wide...
Summary The large, plant-specific protein GIGANTEA (GI) is involved in many physiological processes, mediating rhythmic, post-translational regulation part through circadian and light of GI RNA expression. binds several proteins implicated the clock, control photoperiodic flowering, abiotic stress responses, has co-chaperone activity. By extension, further interaction partners might mediate less well-understood roles but number rhythmicity these interactors unknown. Here, we seek potential a...
Abstract Adventitious rooting is critical to the propagation, breeding, and genetic engineering or editing of trees. The capacity for plants undergo these processes highly heritable; however, basis its variation largely uncharacterized. To identify regulators processes, we performed a genome-wide association study (GWAS) using 1,148 genotypes Populus trichocarpa . GWAS are often limited by abilities researchers collect precise phenotype data on high-throughput scale; help overcome this...