A5016606500- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- CRISPR and Genetic Engineering
University of California, Berkeley
2025
University of Washington
2017
CYCLING DOF FACTOR 1 (CDF1) and its homologs play an important role in the floral transition by repressing expression of activator genes such as CONSTANS (CO) FLOWERING LOCUS T (FT) Arabidopsis. The day-length-specific removal CDF1-dependent repression is a critical mechanism photoperiodic flowering. However, which CDF1 represses CO FT transcription remained elusive. Here we demonstrate that Arabidopsis CDF proteins contain non-EAR motif-like conserved domains required for interaction with...
TnpB is an evolutionarily diverse family of RNA-guided endonucleases associated with prokaryotic transposons. Due to their small size and putative evolutionary relationship Cas12s, holds significant potential for genome editing mechanistic exploration. However, most TnpBs lack robust gene-editing activity, unbiased profiling mutational effects on activity has not been experimentally explored. Here, we mapped comprehensive sequence-function landscapes a ribonucleoprotein discovered many...