A5035950294- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Fungal and yeast genetics research
- Ubiquitin and proteasome pathways
- Genetic Mapping and Diversity in Plants and Animals
- Plant Virus Research Studies
- 14-3-3 protein interactions
- Plant Physiology and Cultivation Studies
- Microbial Metabolic Engineering and Bioproduction
- Plant Gene Expression Analysis
- Plant Genetic and Mutation Studies
- Plant Parasitism and Resistance
- Plant Micronutrient Interactions and Effects
- DNA Repair Mechanisms
- Polysaccharides and Plant Cell Walls
- Polymer-Based Agricultural Enhancements
- Plant-Microbe Interactions and Immunity
- Light effects on plants
- Microfluidic and Bio-sensing Technologies
Okayama University of Science
2023
Advanced Science Research Center
2023
RIKEN Center for Sustainable Resource Science
2011-2021
Tsurumi University
2010
Japan Science and Technology Agency
2010
Plant (United States)
2010
RIKEN Center for Integrative Medical Sciences
2004-2006
Wako University
2005
Yokohama City University
2005
RIKEN
2004-2005
Abscisic acid (ABA) is one of the most important phytohormones involved in abiotic stress responses, seed maturation, germination, and senescence. ABA predominantly produced vascular tissues exerts hormonal responses various cells, including guard cells. Although require extrusion from ABA-producing cells an intercellular signaling pathway, transport mechanisms through plasma membrane remain unknown. Here we isolated ATP-binding cassette (ABC) transporter gene, AtABCG25 , Arabidopsis by...
High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome in Arabidopsis thaliana. These exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180–base pair repeats. Unexpectedly, within centromeres not merely structural but also encoded several expressed genes. The flanking were densely populated by repetitive elements yet experienced normal levels recombination. genetically...
We analyzed the spatial expression pattern of Arabidopsis thaliana adenosine phosphates-isopentenyltransferase genes (AtIPT1, AtIPT3 to AtIPT8) and effect inorganic nitrogen sources on their regulation. In mature plants, AtIPTs were differentially expressed in various tissues including roots, leaves, stems, flowers siliques. transgenic seedlings expressing a gene for green fluorescent protein (GFP) driven by AtIPT promoters, AtIPT1::GFP was predominantly vascular stele AtIPT3::GFP phloem...
Abstract The phytohormone abscisic acid (ABA) regulates physiologically important developmental processes and stress responses. Previously, we reported on Arabidopsis (Arabidopsis thaliana) L. Heynh. ahg mutants, which are hypersensitive to ABA during germination early growth. Among them, ABA-hypersensitive germination3 (ahg3) showed the strongest hypersensitivity. In this study, found that AHG3 gene is identical AtPP2CA, encodes a protein phosphatase 2C (PP2C). Although AtPP2CA has been be...
There are three iron superoxide dismutases in Arabidopsis thaliana: FE SUPEROXIDE DISMUTASE1 (FSD1), FSD2, and FSD3. Their biological roles chloroplast development unknown. Here, we show that FSD2 FSD3 play essential early development, whereas FSD1, which is found the cytoplasm, does not. An fsd2-1 fsd3-1 double mutant had a severe albino phenotype on agar plates, fsd2 fsd3 single knockout mutants pale green phenotypes. Chloroplast was arrested young seedlings of mutant. The plants were...
Summary The Spo11 protein is a eukaryotic homologue of the archaeal DNA topoisomerase VIA subunit (topo VIA). In archaea it involved, together with its B VIB), in replication. However, most eukaryotes, including yeasts, insects and vertebrates, instead have single gene for Spo11/topo no homologues topo VIB. these organisms, mediates double‐strand breaks that initiate meiotic recombination. Many plant species, contrast to other three one Arabidopsis, AtSPO11‐1, AtSPO11‐2 AtSPO11‐3, all share...
Summary More than 10 000 transposon‐tagged lines were constructed by using the Activator ( Ac )/ Dissociation Ds ) system in order to collect insertional mutants as a useful resource for functional genomics of Arabidopsis . The flanking sequences element 11 800 independent determined high‐throughput analysis semi‐automated method. sequence data allowed us map unique insertion site on genome each line. 7566 is inserted or close coding regions, potentially affecting function 5031 25 genes....
The MS/MS spectral tag (MS2T) library-based peak annotation procedure was developed for informative non-targeted metabolic profiling analysis using LC-MS. An MS2T library of Arabidopsis metabolites created from a set spectra acquired the automatic data acquisition function mass spectrometer. By this library, we obtained structural information detected peaks in profile without performing additional analysis; achieved by searching corresponding accession library. In case tissues containing...
In plants, water vapour is released into the atmosphere through stomata in a process called transpiration. Abscisic acid (ABA) key phytohormone that facilitates stomatal closure its action on guard cells. Recently, ATP-binding cassette (ABC) transporter genes, AtABCG25 and AtABCG40, were shown to be involved ABA transport responses. However, functions of many other AtABCG family genes are still unknown. Here, we identified another ABCG gene (AtABCG22) required for regulation Arabidopsis. The...
To identify candidate genes involved in Arabidopsis flavonoid biosynthesis, we applied transcriptome coexpression analysis and independent component analyses with 1388 microarray data from publicly available databases. Two glycosyltransferases, UGT79B1 UGT84A2 were found to cluster anthocyanin biosynthetic genes. Anthocyanin was drastically reduced ugt79b1 knockout mutants. Recombinant protein converted cyanidin 3-O-glucoside 3-O-xylosyl(1→2)glucoside. recognized 3-O-glucosylated...
Abstract Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo . In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, synthesized the mitochondria molecular mechanisms underlying transport into chloroplasts unknown. Here we show that AtPHT4;4, a member of phosphate transporter 4 family Arabidopsis thaliana , functions as transporter. vitro analysis shows proteoliposomes containing purified AtPHT4;4...
Summary Arabidopsis carries three receptor genes for the phytohormone gibberellin (GA), AtGID1a , AtGID1b and AtGID1c . Expression of each gene in rice gid1‐1 mutant GA receptors causes reversion its severely dwarfed phenotype insensitivity to a normal level, even though loss‐of‐function shows no clear ( Nakajima et al. 2006 ). In this paper, we report functional redundancy specificity AtGID1 by analyzing multiple mutants loss function. Seeds double knockout atgid1a atgid1b atgid1c...
Abstract Abscisic acid (ABA) is a phytohormone that responds to environmental stresses, such as water deficiency. Recent studies have shown ABA biosynthetic enzymes are expressed in the vascular area under both nonstressed and water-stressed growth conditions. However, specific cells vasculature involved biosynthesis not been identified. Here, we detected expression of two genes encoding enzymes, ABSCISIC ACID DEFICIENT2 ALDEHYDE OXIDASE3, phloem companion tissues. Furthermore, identified an...
Human DNA variation is currently a subject of intense research because its importance for studying human origins, evolution, and demographic history association studies complex diseases. A ∼10-kb region on chromosome 1, which contains only four small exons (each <155 bp), was sequenced 61 humans (20 Africans, 20 Asians, 21 Europeans) 1 chimpanzee, gorilla, orangutan. We found 52 polymorphic sites among the 122 sequences 382 variant human, orangutan sequences. For introns (8,991 nucleotide...
Accumulating evidence suggests that mRNA degradation systems are crucial for various biological processes in eukaryotes. Here we provide an system is associated with some plant hormones and stress responses plants. We analysed a novel Arabidopsis abscisic acid (ABA)-hypersensitive mutant, ahg2-1, showed ABA hypersensitivity not only germination, but also at later developmental stages, displayed pleiotropic phenotypes. found ahg2-1 accumulated more endogenous seeds mannitol-treated plants...
Mutant lines covering all Arabidopsis genes allow us to pursue systematic functional genomics. A comprehensive phenotype description, called a phenome, is highly sought after in the profiling of -omics data. We selected 4000 transposon-insertional with transposon insertions their gene-coding regions, and systematically observed visible each line. For first 3 weeks germination, plants were grown on agar plates juvenile phenotypes recorded. Then transferred soil recorded at growth stage. About...
Despite the importance of extracellular events in cell wall organization and biogenesis, mechanisms related factors are largely unknown. We isolated an allele shaven3 (shv3) mutant Arabidopsis thaliana, which exhibits ruptured root hair cells during tip growth. SHV3 encodes a novel protein with two tandemly repeated glycerophosphoryl diester phosphodiesterase-like domains glycosylphosphatidylinositol anchor, several its paralogs found Arabidopsis. Here, we report detailed characterization...
We transposed Dissociation(Ds) elements from three start loci on chromosome 5 in Arabidopsis (Nossen ecotype) by using a local transposition system. determined partial genomic sequences flanking the Dselements and mapped elements' insertion sites 1,173 lines comparison with published sequence. Most of contained single copy Ds element. One-half 5; particular, “hot spots” near were clearly observed. In other lines, across chromosomes. found hot spots at tops chromosomes 2 4, nucleolus...