A5036079911- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Alzheimer's disease research and treatments
- Genetic Neurodegenerative Diseases
- Parkinson's Disease Mechanisms and Treatments
- Amino Acid Enzymes and Metabolism
- Advanced Chemical Physics Studies
- Fullerene Chemistry and Applications
- Sulfur Compounds in Biology
- Nuclear Physics and Applications
- Carbon Nanotubes in Composites
- Neurological disorders and treatments
- RNA and protein synthesis mechanisms
- Medical Imaging Techniques and Applications
- Photochemistry and Electron Transfer Studies
- Metabolism and Genetic Disorders
- Analytical chemistry methods development
- Graphene research and applications
- Ginseng Biological Effects and Applications
- Corneal surgery and disorders
- Alkaloids: synthesis and pharmacology
- Liver physiology and pathology
- HIV-related health complications and treatments
- Peripheral Neuropathies and Disorders
Bruker (United States)
2011-2021
Kanagawa University
2019
Nihon University
1997-2009
Tohoku University
1998-2004
Tokyo Metropolitan Institute of Gerontology
2000
Tokyo Metropolitan Institute of Medical Science
2000
Amyloid β (Aβ) deposition in the brain is an early and invariable feature of Alzheimer's disease (AD). The Aβ peptides are composed about 40 amino acids generated from amyloid precursor proteins (APP), by β- γ-secretases. distribution individual brains aged people, those suffering AD cerebral angiopathy (CAA), not fully characterized. We employed matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) to illustrate spatial a broad range species human autopsied...
DNA is packaged as chromatin in the interphase nucleus. During mitosis, fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal into daughter cells. Despite >1 century research on information regarding higher order structure chromosomes limited, and it still not clear proteins involved further folding fiber chromosomes. To obtain a global view proteins, we performed proteome analyses three types isolated human We first show results...
Significance Dimerization of HIV-1 protease (PR) plays a critical role in the replication HIV-1. Darunavir (DRV) inhibits not only proteolytic activity but also PR dimerization. The present study shows that dimerization process undergoes two steps and DRV first step by binding to monomers one-to-one molar ratio. demonstrates binds transframe precursor protein, indicating DRV’s monomer is involved Gag-Pol autoprocessing inhibition. To our knowledge, report represents demonstration two-step...
Abstract Apart from nutrients required for the brain, there has been no report that naturally occurring peptides can cross blood-brain barrier (BBB). The aim of this study was to identify BBB-transportable using in situ mouse perfusion experiments. Based on structural features Gly- N -methylated Gly (Gly-Sar), a reported compound, 18 dipeptides were synthesized, and perfused brain two minutes. Among synthesized dipeptides, Gly-Sar, Gly-Pro, Tyr-Pro transported across BBB with K i values 7.60...
Introduction: Amyloid-beta (Aβ) pathology is the precipitating histopathological characteristic of Alzheimer's disease (AD). Although formation amyloid plaques in human brains suggested to be a key factor initiating AD pathogenesis, it still not fully understood upstream events that lead Aβ plaque and its metabolism inside brains. Methods: Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) has been successfully introduced study brain tissue both mouse models...
Abstract Proteome analysis by 2‐DE and PMF MALDI‐TOF MS was performed on human amnion amniotic fluid at term. Ninety‐two soluble nineteen membrane proteins were identified from amnion. Thirty‐five fluid. Calgranulin A B found in all patients infected with Ureaplasma urealyticum , but not any of the without infection, indicating that they are potential markers intrauterine infection. Identity calgranulin confirmed MALDI‐TOF/TOF MS. This study represents first extensive proteome term...
Abstract In transsulfuration reaction catalyzed by rat mercaptopyruvate sulfurtransferase (MST), a stable persulfide is formed at the catalytic site cysteine Cys(247) as intermediate. The outer sulfur atom donated substrate, thiosulfate, or mercaptopyruvate. MST serves thioredoxin-dependent antioxidant possessing self-regulated enzymatic activity. After oxidation of persulfurated treatment with hydrogen peroxide, mass spectrometric analysis showed that oxidized to form Cys-thiosulfenate...
Abstract Docosahexaenoic acid (DHA) and ultra‐long‐chain polyunsaturated fatty acids (ULC–PUFAs) are uniquely enriched in membrane phospholipids of retinal photoreceptors. Several studies have shown that di‐DHA‐ ULC–PUFA‐containing photoreceptors an important role maintaining normal visual function; however, the molecular mechanisms underlying synthesis enrichment these unique lipids retina, their specific roles function remain unclear. Long‐chain acyl‐coenzyme A (CoA) synthetase 6 (ACSL6)...
Abstract Fuchs endothelial corneal dystrophy (FECD) is a slowly evolving, bilateral disease of the endothelium, characterized by an abnormal accumulation extracellular matrix (ECM) in basement membrane (Descemet’s membrane, DM). This results formation small round excrescences, called guttae, and progressive disappearance cells. In intermediate stage, numerous guttae create significant optical aberrations, late loss function leads to permanent edema. The molecular components have not been...
The matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometric technique (IMS) offered a new breakthrough perspective in the analysis of drug abuse forensic science; however, it only produced barcode-like images, semi-quantitative analysis. In order to develop intermittent monitoring by this IMS for and medical sciences, is important quantitatively measure contents longitudinally sliced hair sections. We developed quantitative spectrometry (QIMS) nicotine (NC) hairs...
Fuchs endothelial corneal dystrophy (FECD) is a bilateral, progressive disease characterized by the formation of extracellular matrix (ECM) excrescences called guttae. This study integrated proteomic and transcriptomic analyses to elucidate molecular composition spatial organization ECM proteins in Descemet membrane (DM) FECD patients. Through shotgun proteomics FECD-derived DM specimens RNA sequencing data from (n = 10) control 7) cells, we identified 19 significantly upregulated molecules...
Abstract The differential diagnosis of Parkinson's disease and multiple system atrophy can be challenging, especially in the early stages diseases. We developed a proteomic profiling strategy for parkinsonian diseases using mass spectrometry analysis magnetic‐bead‐based enrichment cerebrospinal fluid peptides/proteins subsequent multivariate statistical analysis. Cerebrospinal was obtained from 37 patients diagnosed with disease, 32 atrophy, 26 other neurological as controls. samples were...
Flavan-3-ols, procyanidins and their monomers are major flavonoids present in peanuts that show a wide range of biological properties health benefits, based on potent antioxidant activity. Procyanidin oligomers, especially A-type, reportedly abundant peanut skin; however, localization the raw testa remains poorly understood. Therefore, we performed matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to investigate flavan-3-ols testa. 1,5-Diaminonaphthalene was...
To use a new, unbiased biomarker discovery strategy to obtain and assess proteomic data from cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS)-related disorders.CSF protein profiles were analyzed 107 either MS-related disorders (including relapsing remitting MS [RRMS], primary progressive [PPMS], anti-aquaporin4 antibody seropositive-neuromyelitis optica spectrum disorder [SP-NMOSD], seronegative-NMOSD long cord lesions on spinal magnetic resonance imaging [SN-NMOSD]),...
The neuropathology of Alzheimer's disease (AD) is characterized by the accumulation and aggregation amyloid β (Aβ) peptides into extracellular plaques brain. Aβ peptides, composed 40 amino acids, are generated from precursor proteins (APP) β- γ-secretases. deposited not only in cerebral parenchyma but also leptomeningeal vessel walls, known as angiopathy (CAA). While a variety were identified, detailed production distribution individual pathological tissues AD CAA have been fully addressed....
Pathological hallmark of Alzheimer's disease (AD) is characterized by the accumulation and aggregation amyloid β (Aβ) peptides into extracellular plaques brain. Clarification process how soluble Aβ starts to assemble fibrils an essential step in elucidating pathogenesis AD. In our previous study, proteoforms including full-length Aβ40 Aβ42/43 with N- C-terminal truncated forms were visualized postmortem brains from AD patients matrix-assisted laser desorption/ionization-based mass...
2-Aminobenzamide-derivatized oligosaccharides were separated by three lectin column chromatographies and then subjected to matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for structural characterization of the carbohydrates. The combination sequential exoglycosidase digestion MALDI-TOF MS greatly facilitates monosaccharide sequencing is more feasible than size-exclusion chromatography in terms time consumed laboriousness procedure. By this...
Neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disorder, characterized by the presence of eosinophilic inclusions (NIIs) within nuclei central and peripheral nervous system cells. This study aims to identify components NIIs, which have been difficult analyze directly due their insolubility. In order establish method we first analyzed huntingtin inclusion-rich fraction obtained from brains Huntington model mice. Although sequence with expanded polyglutamine could not be...
Plants release specialized (secondary) metabolites from their roots to communicate with other organisms, including soil microorganisms. The spatial behavior of such around these can help us understand roles for the communication; however, currently, they are unclear because soil-based studies complex. Here, we established a multimodal metabolomics approach using imaging mass spectrometry (IMS) and liquid chromatography-tandem (LC-MS/MS) spatially assign under laboratory conditions agar. In...
The dimeric C70 oxides were synthesized from C70/C70O mixed powder by hydrothermal treatment at 373 K for 12 h in aqueous NaOH solution. In this treatment, nucleophilic OH- ions are abundant and addition reactions considered to synthesize fullerene oxides. yield was estimated as ca. 2−3% summing up each isomer also produced polyoxides, it is lower than that of C120O. Three isomers C140O isolated using High Performance Liquid Chromatography (HPLC) characterized with UV−vis, FT-IR, 13C NMR...