The purpose of this study was to discover the effects soybean β-conglycinin (7S-globulin) and glycinin (11S-globulin) on serum lipid levels metabolism in livers normal genetically obese mice. Male (ICR) (KK-Ay) mice were fed ad libitum high fat diets for two weeks, followed by a 2-week restriction diet (2 g diet/mouse/day) containing 20% casein, β-conglycinin, or glycinin, then sacrificed immediately. Serum triglyceride (TG), glucose, insulin β-conglycinin-fed lower than casein- glycinin-fed...

Abstract Apart from nutrients required for the brain, there has been no report that naturally occurring peptides can cross blood-brain barrier (BBB). The aim of this study was to identify BBB-transportable using in situ mouse perfusion experiments. Based on structural features Gly- N -methylated Gly (Gly-Sar), a reported compound, 18 dipeptides were synthesized, and perfused brain two minutes. Among synthesized dipeptides, Gly-Sar, Gly-Pro, Tyr-Pro transported across BBB with K i values 7.60...

In this study, we compared the intestinal absorption from different sources with same amino acid composition (protein, peptide, and mixture) by measuring changes in serum concentrations of various acids. Peptides used study were prepared enzymatic digestion 11S globulin (a major component soy protein). Twelve normal male adults asked to drink beverages containing 12.5g test sample, representing 5% suspension each following three preparations : globulin, an mixture equivalent composition....

Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays an important role in cognitive abilities, including memory and learning. We demonstrated that soybean protein hydrolysate (SPH) diet suppresses age-related decline via upregulation BDNF mouse model senescence. Our purpose was to identify novel bioactive peptides SPH, which enhance expression. treated primary astrocytes with SPH as well its positively charged chromatographic fraction. Significant increases...

In this study, HepG2 cells were treated with short peptides (7S-peptides) derived from highly purified soybean beta-conglycinin (7S), which was free lipophilic protein, and the effect of peptide treatment on lipid metabolism determined. 7S-peptide suppressed secretion apolipoprotein B-100 into medium. The 7S-peptides also incorporation (3)H-glycerol (14)C-acetate triacylglyceride but not major phospholipids, such as phosphatidylcholine phosphatidylethanolamine. Additionally, synthesis...

Purpose: This study compared the effects of a combination soy peptide supplementation and exercise with those only, on cognitive function elderly adults. Patients methods: randomized, non-blinded, controlled clinical trial included 67 participants aged 60 years or more non-cognitive dysfunction who were divided into two groups according to intervention method: an group (Ex group, n = 36) plus nutrition (Ex+Nt 31). The Ex completed memory training activity for 15 mins aerobic 45 once week 90...

The formation of cholic acid and chenodeoxycholic through cleavage the side chains CoA esters 3α, 7α, 12α-trihydroxy-5β-cholestan-26-oic 7α-dihydroxy-5β-cholestan-26-oic is believed to occur in peroxisomes. Recently, we found a new peroxisomal enzyme, D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein, suggested that this protein responsible for conversion 12α-trihydroxy-5β-cholest-24-en-26-oyl-CoA 7α-dihydroxy-5β-cholest-24-en-26-oyl-CoA their...

Malonyl isoflavone glucosides are water-soluble derivatives of soybean hypocotyls. This study compared the hydrolysis and absorption malonyl nonmalonyl in rats. Plasma concentrations isoflavones were measured after oral administration or glucosides. After fasting, duodenum, jejunum, ileum, colon excised, homogenates prepared. The extent each glucoside by intestinal homogenate was measured. levels aglycones, such as daidzein glycitein, higher rats administered than those area under curve...

We purified catalase‐2 of the nematode Caenorhabditis elegans and identified peroxisomes in this organism. The C. were not detectable by cytochemical staining using 3,3′‐diaminobenzidine, a commonly used method depending on peroxidase activity peroxisomal catalase at pH 9 which genuine peroxidases are inactive. cDNA sequences predict two catalases very similar to each other throughout molecule, except for short C‐terminal sequence; (500 residues long) carries targeting signal 1‐like sequence...

Soybean (Glycine max L.) storage proteins are composed of two major components, beta-conglycinin and glycinin, corresponding to 7S 11S globulins, respectively. Recently, soybean (7S globulin) has been reported show beneficial functions in animals human. To date, there is no method for the precise quantification processed food products or seeds. We report here a novel this purpose. At first, antibodies specifically reactive subunits were prepared. And then, direct enzyme-linked immunosorbent...

To examine whether edible peptide intake affects neurotransmitter metabolism in the brain, we evaluated effect of peptides derived from soy proteins or fish collagen on free amino acids and monoamines mouse brain. Ingestion led to markedly higher levels tyrosine, a catecholamine precursor, serum, cerebral cortex compared those following ingestion vehicle alone peptides. Soy also effectively increased 3-methoxy-4-hydroxyphenylethyleneglycol normetanephrine, principal metabolites...

We used a DNA microarray to compare the gene expression profiles in liver among three groups of mice fed diet containing 5% royal jelly (RJ), RJ stored at 40 degrees C for 7 d (40-7d RJ) or control which provides same total energy as RJ. Expression 267 genes was increased decreased by 1.8-fold more animals given 14 compared with diet, though serum cholesterol, triglyceride, phospholipid, glucose, insulin and leptin levels were unaffected. Many involved cell growth, signal transduction,...

We cloned a full‐length cDNA of the nematode Caenorhabditis elegans that encodes 44‐kDa protein (P‐44, 412 residues) similar to sterol carrier x (SCPx). Mammalian SCPx is bipartite protein: its 404‐residue N‐terminal and 143‐residue C‐terminal domains are 3‐ketoacyl‐CoA thiolase identical precursor 2 (SCP2; also termed non‐specific lipid‐transfer protein), respectively. P‐44 has 56% sequence identity domain but lacks SCP2 sequence. Northern blot analysis revealed only single mRNA species 1.4...

Both the linear plasmids, pDHL1 (8·4 kb) and pDHL2 (9·2 kb), of Debaryomyces hansenii TK require presence a third plasmid pDHL3 (15·0 in same host cell for their replication. A 3·5 kb Bam HI-PstI fragment strongly hybridized by Southern analysis to NcoI-AccI pDHL2, suggesting importance this conserved region replication two smaller pDHL plasmids. The 4·2 containing above was cloned sequenced. results showed that encodes protein 1000 amino acid residues, having strong similarity DNA...

We examined the effect of orally administering l-Ser-l-Tyr (SY) dipeptide on brain a serine deficiency disease model mouse to attain efficient delivery l-Tyr and l-Ser into brain. Oral SY administration increased level more efficiently than with same intake dose, but did not significantly affect when compared administration.

Soy peptide, when consumed as a functional food, has been reported to improve cognitive function. This study aimed verify the combined effect of soy peptide supplementation and exercise on function among community-dwelling older adults in Japan.In this population-based, non-blinded randomized controlled trial, 72 who were independent activities daily living randomly assigned an "exercise plus nutrition" program (Ex + Nt group, n = 36) or 36). For 3 months, both groups participated training...

We examined the expression and localization of type‐II 3‐oxoacyl‐CoA thiolase in nematode Caenorhabditis elegans . Type‐II acts on esters with a methyl group at α carbon, whereas conventional thiolases do not. Mammalian thiolase, which is also termed sterol carrier protein x (SCPx) or SCP2/3‐oxoacyl‐CoA located peroxisomes involved phytanic acid degradation most probably bile synthesis. The enzyme lacks SCP2 domain, carries peroxisomal‐targeting signal, but produces acids cell‐free system....

The effect of soy and casein peptide intake on the metabolism amino acids monoamine neurotransmitters in serum brain were examined C57BL/6 mice. Acute oral administration (0.026 g/30 g body weight) caused a notable increase tyrosine, catecholamine precursor, cerebral cortex, whereas at same dose led to an tyrosine serum, but not cortex. In addition also effective augmentation 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG), principal metabolite noradrenaline, significant facilitation...

Soy peptides were produced by microbial protease digestion of soy proteins. Under an acidic condition (pH 3.8), peptide solution (5% w/v) yielded sediment during chilled storage. The object the present study was to identify in order elucidate sedimentation mechanism. Peptides remaining after storage had a molecular mass 200 – 5,000 Dalton (Da), based on elution volume obtained from size exclusion chromatography. However, extracted using 30% acetonitrile-0.1% trifluoroacetic acid larger than...