A5076764999- Sulfur Compounds in Biology
- Amino Acid Enzymes and Metabolism
- Neuroscience of respiration and sleep
- Folate and B Vitamins Research
- Neuroscience and Neuropharmacology Research
- Obstructive Sleep Apnea Research
- Aortic aneurysm repair treatments
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Nitric Oxide and Endothelin Effects
- Alzheimer's disease research and treatments
- Alcohol Consumption and Health Effects
- Pharmacogenetics and Drug Metabolism
- Peripheral Artery Disease Management
- Pharmacological Effects of Natural Compounds
- Polyamine Metabolism and Applications
- RNA Interference and Gene Delivery
- Mast cells and histamine
- Virus-based gene therapy research
- Drug Transport and Resistance Mechanisms
- Molecular Sensors and Ion Detection
- Aldose Reductase and Taurine
- Vascular Procedures and Complications
- Biochemical effects in animals
- Infectious Aortic and Vascular Conditions
Sanyo-Onoda City University
2019-2024
Miyagi Prefectural Hospital Organization
2024
Pharmac
2020-2023
Uji Hospital
2023
Fukuoka University
1991-2020
National Center of Neurology and Psychiatry
2010-2019
National Institute for Materials Science
2011-2019
NTL Institute for Applied Behavioral Science
2011-2019
National Neuroscience Institute
2010-2019
Shenzhen Institutes of Advanced Technology
2019
Hydrogen sulfide (H2S), which is well known as a toxic gas, produced endogenously from L-cysteine in mammalian tissues. H2S present at relatively high levels the brain, suggesting that it has physiological function. Two other gases, nitric oxide and carbon monoxide, are also have been proposed neuronal messengers brain. In this work we show following: (1) an H2S-producing enzyme, cystathionine beta-synthase (CBS), highly expressed hippocampus; (2) CBS inhibitors hydroxylamine...
The recent discovery that hydrogen sulfide (H(2)S) is an endogenously produced gaseous second messenger capable of modulating many physiological processes, much like nitric oxide, prompted us to investigate the potential H(2)S as a cardioprotective agent. In current study, we demonstrate delivery at time reperfusion limits infarct size and preserves left ventricular (LV) function in vivo model myocardial ischemia-reperfusion (MI-R). This observed cytoprotection associated with inhibition...
Abstract: 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) reduction is one of the most frequently used methods for measuring cell proliferation and neural cytotoxicity. It widely assumed that MTT reduced by active mitochondria in living cells. By using isolated from rat brain B12 cells, we indeed found malate, glutamate, succinate support mitochondria. However, data presented this study do not exclusive role intact Using a variety approaches, cells confined to...
Hydrogen sulfide (H(2)S) is a synaptic modulator as well neuroprotectant. Currently, pyridoxal-5'-phosphate (PLP)-dependent cystathionine beta-synthase (CBS) thought to be the major H(2)S-producing enzyme in brain. We recently found that brain homogenates of CBS-knockout mice, even absence PLP, produce H(2)S at levels similar those wild-type suggesting presence another enzyme. Here we show 3-mercaptopyruvate sulfurtransferase (3MST) combination with cysteine aminotransferase (CAT) produces...
Hydrogen sulfide (H2S), which is a well-known toxic gas, found in relatively high concentrations the brain. Although neuromodulatory role of H2S has been demonstrated, little known its other biological functions. Here we show that protects primary cultures neurons from death well-studied model oxidative stress caused by glutamate, process called glutamate toxicity--or oxytosis. We increases glutathione levels, normally decrease during cell cascade, enhancing activity gamma-glutamylcysteine...
Hydrogen sulfide (H(2)S) is a synaptic modulator as well neuroprotectant in the brain. We recently showed that H(2)S protects neurons from oxidative stress by increasing levels of glutathione (GSH), major cellular antioxidant, more than twice control through enhancing cystine transport. Here we show enhances transport cysteine to increase GSH production and redistribute localization mitochondria. The efficiency enhanced even greater fourfold under glutamate. reinstated fetal brain decreased...
Hydrogen sulfide (H(2)S) has recently been identified as a biological response modifier. Here, we report the design and synthesis of novel fluorescence probe for H(2)S, HSip-1, utilizing azamacrocyclic copper(II) ion complex chemistry to control fluorescence. HSip-1 showed high selectivity sensitivity its potential applications was confirmed by employing it imaging H(2)S in live cells.
Hydrogen sulfide (H2S) has been recognized as a smooth muscle relaxant. Cystathionine γ-lyase, which is localized to muscle, thought be the major H2S-producing enzyme in thoracic aorta. Here we show that 3-mercaptopyruvate sulfurtransferase (3MST) and cysteine aminotransferase (CAT) are vascular endothelium aorta produce H2S. Both 3MST CAT were endothelium. Lysates of endothelial cells produced H2S from α-ketoglutarate. The present study provides new insight into production release relaxant
Hydrogen sulfide (H2S) is recognized as a neuromodulator well neuroprotectant in the brain. H2S can be produced from cysteine by enzymes such cystathionine beta-synthase. However, mechanism for releasing under physiologic conditions has not been identified. Here we show that released bound sulfur, an intracellular store of neurons and astrocytes mice rats presence concentrations endogenous reducing substances glutathione cysteine. The highest pH to release another sulfur store, acid-labile...
Accumulating evidence shows that hydrogen sulfide (H2S) has a variety of physiological functions. H2S is produced from cysteine by 3 sulfurtransferases. H2S, in turn, generates polysulfides, the functions which are not well understood. induces Ca2+ influx astrocytes, type glia. However, receptor mediates response been identified. Here, we have shown polysulfides induce activating transient potential (TRP)A1 channels rat astrocytes (EC50 91 nM, Hill coefficient value 1.77±0.26) and maximum...
Hydrogen sulfide (H2S) has been recognized as a physiological mediator with variety of functions. It regulates synaptic transmission, vascular tone, inflammation, transcription, and angiogenesis; protects cells from oxidative stress ischemia-reperfusion injury; promotes healing ulcers.In addition to cystathionine β-synthase γ-lyase, 3-mercaptopyruvate sulfurtransferase along cysteine aminotransferase was recently demonstrated produce H2S. Even in bacteria, H2S produced by these enzymes...
H2S (hydrogen sulfide) has recently been recognized as a signalling molecule well cytoprotectant. We demonstrated that 3MST (3-mercaptopyruvate sulfurtransferase) produces from 3MP (3-mercaptopyruvate). Although reducing substance is required for an intermediate persulfide at the active site of to release H2S, not identified. In present study we show Trx (thioredoxin) and DHLA (dihydrolipoic acid) associate with H2S. Other substances, such NADPH, NADH, GSH, cysteine CoA, did have any effect...
Abstract Hydrogen polysulfides (H 2 S n ) have a higher number of sulfane sulfur atoms than hydrogen sulfide S), which has various physiological roles. We recently found H in the brain. induced some responses previously attributed to but with much greater potency S. However, and its producing enzyme were unknown. Here, we detected 3 S, produced from 3-mercaptopyruvate (3 MP) by sulfurtransferase (3MST), High performance liquid chromatography fluorescence detection (LC-FL) tandem mass...