A5049944942- Molecular Sensors and Ion Detection
- Sulfur Compounds in Biology
- Nanoplatforms for cancer theranostics
- Luminescence and Fluorescent Materials
- Click Chemistry and Applications
- Advanced biosensing and bioanalysis techniques
- Lanthanide and Transition Metal Complexes
- Photoreceptor and optogenetics research
- Photodynamic Therapy Research Studies
- Peptidase Inhibition and Analysis
- Advanced Fluorescence Microscopy Techniques
- Analytical Chemistry and Sensors
- Photochromic and Fluorescence Chemistry
- Cancer, Hypoxia, and Metabolism
- Neuroscience of respiration and sleep
- Amino Acid Enzymes and Metabolism
- bioluminescence and chemiluminescence research
- Advanced MRI Techniques and Applications
- Polyamine Metabolism and Applications
- Porphyrin and Phthalocyanine Chemistry
- Biotin and Related Studies
- Chemical Synthesis and Analysis
- Folate and B Vitamins Research
- Nanoparticle-Based Drug Delivery
- Medical Imaging Techniques and Applications
Keio University
1984-2025
The University of Tokyo
2014-2023
Kumamoto University
2014-2020
Bunkyo University
2011-2020
Showa Pharmaceutical University
2018
Japan Science and Technology Agency
2008-2015
The Open University of Japan
2015
Massachusetts General Hospital
2014
Washington State University
2014
Harvard University
2014
Hydrogen sulfide (H(2)S) has recently been identified as a biological response modifier. Here, we report the design and synthesis of novel fluorescence probe for H(2)S, HSip-1, utilizing azamacrocyclic copper(II) ion complex chemistry to control fluorescence. HSip-1 showed high selectivity sensitivity its potential applications was confirmed by employing it imaging H(2)S in live cells.
A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive selective detection of HOCl in real time. MMSiR its oxidized product SMSiR have excellent properties, including pH-independence fluorescence, high resistance autoxidation photobleaching, good tissue penetration NIR emission. The value confirmed by real-time imaging phagocytosis using a microscope. wsMMSiR, more hydrophilic derivative permitted effective vivo...
Detection of chelatable zinc (Zn2+) in biological studies has attracted much attention recently, because Zn2+ plays important roles many systems. Lanthanide complexes (Eu3+, Tb3+, etc.) have excellent spectroscopic properties for applications, such as long luminescence lifetimes the order milliseconds, a large Stoke's shift >200 nm, and high water solubility. Herein, we present design synthesis novel lanthanide sensor molecule, [Eu-7], detecting Zn2+. This europium (Eu3+) complex employs...
The absorption and emission wavelengths of group 14 pyronines rhodamines, which contain silicon, germanium, or tin at the 10 position xanthene chromophore, showed large bathochromic shifts compared to original owing stabilization LUMO energy levels by σ*-π* conjugation between atom-C (methyl) σ* orbitals a π* orbital fluorophore. These rhodamines retain advantages including high quantum efficiency in aqueous media (Φ(fl) = 0.3-0.45), tolerance photobleaching, water solubility. Group have...
Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis novel fluorescence probe for hydrogen peroxide, utilizing photoinduced electron transfer strategy based on benzil chemistry control fluorescence. The practical value this highly sensitive selective probe, NBzF, was confirmed its application imaging generation live RAW 264.7 macrophages. NBzF also employed cell generated as signaling...
Based on the findings that azo functional group has excellent properties as hypoxia-sensor moiety, we developed hypoxia-sensitive near-infrared fluorescent probes in which a large fluorescence increase is triggered by cleavage of an bond. The were used for imaging hypoxic cells and real-time monitoring ischemia liver kidney live mice.
Reactive oxygen species (ROS) operate as signaling molecules under various physiological conditions, and overproduction of ROS is involved in the pathogenesis many diseases. Therefore, fluorescent probes for visualizing are promising tools with which to uncover molecular mechanisms pathological processes might also be useful diagnosis. Here we describe a novel fluorescence probe, FOSCY-1, operating physiologically favorable near-infrared region. The probe consists two differentially...
We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si–rhodamine scaffold to obtain emission in range suitable for vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency aqueous media. Both antibody-bound free dye exhibited tolerance photobleaching solution. Subcutaneous xenograft tumors were successfully visualized mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody,...
Novel ratiometric, near-infrared fluorescent pH probes with various pKa values have been designed and synthesized on the basis of aminocyanine bearing a diamine moiety, their photochemical properties were evaluated. Under acidic conditions, these showed 46- to 83-nm red shift absorption maximum. This change is sufficiently large permit use as ratiometric probes, reversible, whereas monoamine-substituted aminocyanines irreversible changes because instability under conditions. Furthermore, can...
Abstract Hydrogen sulfide (H 2 S) and hydrogen polysulfides S n , >1) are endogenous regulators of many physiological processes. In order to better understand the symbiotic relationship cellular cross‐talk between H it is highly desirable develop single fluorescent probes which enable dual‐channel discrimination . Herein, we report rational design, synthesis, evaluation first dual‐detection probe DDP‐1 that can visualize with different fluorescence signals. The showed high selectivity...
Let it shine: New hypoxia-sensitive fluorescent probes were developed; they consist of a rhodamine moiety with an azo group directly conjugated to the fluorophore. Because ultrafast conformational change around NN bond, compounds are nonfluorescent under normoxia. However, hypoxia, is reduced, and strongly derivative released. As service our authors readers, this journal provides supporting information supplied by authors. Such materials peer reviewed may be re-organized for online...
We have synthesized and evaluated a series of hydroxymethyl rhodamine derivatives found an intriguing difference intramolecular spirocyclization behavior: the acetylated derivative green (Ac-HMRG) exists as closed spirocyclic structure in aqueous solution at physiological pH, whereas HMRG itself takes open nonspirocyclic structure. Ac-HMRG is colorless nonfluorescent, strongly fluorescent. On basis these findings, we developed general design strategy to obtain highly sensitive fluorescence...
Photodynamic therapy (PDT) utilizes photoirradiation in the presence of photosensitizers to ablate cancer cells via generation singlet oxygen (1O2), but it is important minimize concomitant injury normal tissues. One approach for achieving this use activatable that can generate 1O2 only under specific conditions. Here, we report a novel photosensitizer selectively activated hypoxia, common condition solid tumors. We found introducing an azo moiety into conjugated system seleno-rosamine dye...
Fluorogenic probes for bioimaging have become essential tools life science and medicine, the key to their development is a precise understanding of mechanisms available fluorescence off/on control, such as photoinduced electron transfer (PeT) Förster resonance energy (FRET). Here we establish new molecular design strategy rationally develop activatable fluorescent probes, which exhibit change in response target biomolecules, by controlling twisted intramolecular charge (TICT) process. This...
Superior fluorescence imaging methods are needed for detailed studies on biological phenomena, and one approach that permits precise analyses is time-resolved measurement, which offers a high signal-to-noise ratio. Herein, we describe new system to visualize biomolecules within living samples by means of time-resolved, long-lived luminescence microscopy (TRLLM). In TRLLM, short-lived background scattered light gated out, allowing the be selectively imaged. Usual provides images with...
To improve optical imaging of Ca2+ and to make available a distinct color window for multicolor imaging, we designed synthesized CaSiR-1, far-red near-infrared fluorescence probe Ca2+, using Si-rhodamine (SiR) as the fluorophore well-known chelator BAPTA. This wavelength region is advantageous, affording higher tissue penetration, lower background autofluorescence, phototoxicity in comparison with UV visible range. CaSiR-1 has high off/on ratio over 1000. We demonstrate its usefulness action...
We present a design strategy for fluorescence probes with high off/on activation ratio in the red wavelength region, based on novel fluorescein analogue which O atom at 10 position of xanthene chromophore is replaced Si atom. To demonstrate usefulness this strategy, we designed and synthesized red-fluorescent probe β-galactosidase, showed that it works live HEK293 cells.
We have designed and synthesized a reversible near-infrared (NIR) fluorescence probe, 2-Me TeR, for reactive oxygen species (ROS), utilizing the redox properties of tellurium (Te) atom. TeR is oxidized to fluorescent TeOR by various ROS, while generated quickly reduced in presence glutathione regenerate TeR. This redox-induced NIR-fluorescence response allowed us detect endogenous production ROS subsequent homeostatic recovery intracellular reductive environment hydrogen peroxide-stimulated...
Fluorescence imaging is a powerful tool for the visualization of biological molecules in living cells, tissue slices, and whole bodies, important elucidating phenomena. Furthermore, zinc (Zn(2+)) second most abundant heavy metal ion human body after iron, detection chelatable Zn(2+) studies has attracted much attention. Herein, we present novel, highly sensitive off-on fluorescent chemosensor by using internal charge transfer (ICT) mechanism. The rationale our approach to sensor as follows....
Abstract We adopted a spirocyclization‐based strategy to design γ‐glutamyl hydroxymethyl selenorhodamine green (gGlu‐HMSeR) as photo‐inactive compound that would be specifically cleaved by the tumor‐associated enzyme γ‐glutamyltranspeptidase (GGT) generate potent photosensitizer HMSeR. gGlu‐HMSeR has spirocyclic structure and is colorless does not show marked phototoxicity toward low‐GGT‐expressing cells or normal tissues upon irradiation with visible light. In contrast, HMSeR predominantly...
Here we report the development of fluorogenic substrates for glutathione S-transferase (GST), a multigene-family enzyme mainly involved in detoxification endogenous and exogenous compounds, including drug metabolism. GST is often overexpressed variety malignancies resistance to various anticancer drugs. Despite medical significance this enzyme, no practical fluorescence imaging activity or high-throughput screening inhibitors are yet available. So, set out develop new GST. In preliminary...
In spite of the wide availability various near-infrared (NIR) fluorophores as labeling reagents, there are few functional NIR fluorescent probes for which change in absorption and/or fluorescence spectra upon specific reaction with biomolecules is seen. The widely used photoinduced electron-transfer mechanism unsuitable fluorophores, such tricarbocyanines, because their long excitation wavelength results a small singlet energy. We have reported unique spectral properties amine-substituted...
Near-infrared (NIR) fluorescence probes are especially useful for simple and noninvasive in vivo imaging inside the body because of low autofluorescence high tissue transparency NIR region compared with other wavelength regions. However, existing matrix metalloproteinases (MMPs), which tumor, atherosclerosis, inflammation markers, have various disadvantages, as regards sensitivity. Here, we report a novel design strategy to obtain probe that is rapidly internalized by free diffusion well...