Abstract Hydrogen sulfide (H 2 S) and hydrogen polysulfides S n , >1) are endogenous regulators of many physiological processes. In order to better understand the symbiotic relationship cellular cross‐talk between H it is highly desirable develop single fluorescent probes which enable dual‐channel discrimination . Herein, we report rational design, synthesis, evaluation first dual‐detection probe DDP‐1 that can visualize with different fluorescence signals. The showed high selectivity...

Abstract The design, synthesis, properties, and cell imaging applications of a series 2‐pyridyl disulfide based fluorescent probes (WSP1, WSP2, WSP3, WSP4 WSP5) for hydrogen sulfide detection are reported. strategy is on the dual‐nucleophilicity sulfide. A mediated tandem nucleophilic substitution‐cyclization reaction used to release fluorophores turn fluorescence. showed high sensitivity selectivity over other reactive sulfur species, including cysteine glutathione.

Reactive sulfur species have received considerable attention due to their various biological functions. Among these molecules, hydrogen polysulfides (H2Sn, n > 1) are recently suggested be the actual signaling molecules derived from sulfide (H2S). Hydrogen may also own biosynthetic pathways. The research on H2Sn is rapidly growing. However, detection of still challenging. In this work we report a H2Sn-mediated benzodithiolone formation under mild conditions. Based reaction, specific...

A sulfane sulfur mediated benzodithiolone formation was developed. Based on this reaction, two fluorescent probes (SSP1 and SSP2) for the detection of species (persulfide, polysulfide, elemental sulfur) were prepared evaluated. The showed high selectivity sensitivity to sulfurs. Moreover, SSP2 successfully applied bioimaging sulfurs in living cells.

Near-infrared (NIR) fluorescent dyes with favorable photophysical properties are highly useful for bioimaging, but such still rare. The development of a unique class NIR via modifying the rhodol scaffold fused tetrahydroquinoxaline rings is described. These new showed large Stokes shifts (>110 nm). Among them, WR3, WR4, WR5, and WR6 displayed high fluorescence quantum yields excellent photostability in aqueous solutions. Moreover, their were tunable by easy modifications on phenolic hydroxy...

In this work, an efficient approach for targeting and detecting cancer cells has been developed through the design of assembly fluorescent carbon nanodots folic acid (C-dots–FA), which is endocytosible by overexpressed folate receptor (FR) molecule. The C-dots were prepared a facile microwave pyrolysis method, but their surfaces passivated with 4,7,10-trioxa-1,13-tridecanediamine, so that active amino groups could be engineered further conjugation FA. uptake designed C-dots–FA HeLa cells, as...

Endogenous hydrogen polysulfides (H2Sn; n>1) have been recognized as important regulators in sulfur-related redox biology. H2Sn can activate tumor suppressors, ion channels, and transcription factors with higher potency than H2S. Although are drawing increasing attention, their exact mechanisms of action still poorly understood. A major hurdle this field is the lack reliable convenient methods for detection. Herein we report a H2Sn-mediated benzodithiolone formation under mild conditions....

Hydrogen sulfide (H2S), an important gasotransmitter, can mediate a variety of pathophysiological processes, and H2S-based donors have been intensively explored for the therapy cardiovascular injury, nerve damage intestinal disorders. However, most H2S are not capable simultaneously real-time tracking intracellular delivery, which limits their biological application elucidating specific function H2S. Herein we develop first reactive oxygen species (ROS)-triggered off-on fluorescence donor...

A new strategy that utilizes the interaction between NO and a selenide is reported for fluorescence detection of NO, in which rhodamine B selenolactone serves as model selenide.

A hydrogen polysulfide mediated aziridine ring-opening reaction was discovered. Based on this reaction, a novel H2S(n)-specific chemosensor (AP) developed. AP showed high sensitivity and selectivity for H2S(n). Notably, the fluorescent turn-on product (1) exhibited excellent two-photon photophysical properties, large Stokes shift, solid state luminescent efficiency.

A new near-infrared fluorescence off–on probe with phenyl 2-(benzoylthio)benzoate as the recognition moiety is developed and applied in imaging H₂S_n living cells mice <italic>in vivo</italic>.

Acute lung injury is characterized by neutrophilic inflammation and increased permeability. Thiosulfate a stable metabolite of hydrogen sulfide, gaseous mediator that exerts antiinflammatory effects. Although sodium thiosulfate (STS) has been used as an antidote, the effect STS on acute unknown. The authors assessed effects mice vascular endothelial cells subjected to inflammation.Lung was in challenged with intratracheal lipopolysaccharide or cecal ligation puncture without STS. Effects...

A new resorufin-based spectroscopic probe, 7-(p-acetoxyphenylmethyloxy)-3H-phenoxazin-3-one (1), has been developed for detecting carboxylesterase activity. The probe is designed by introducing p-acetoxyphenylmethyloxy as a bifunctional moiety to resorufin. not only quenches the signal of resorufin, but also serves recognition unit carboxylesterase. As result, prepared latent 1 shows very low background signal, which rather desired achieving sensitive detection. specific cleavage carboxylic...

A new resorufin-based probe is developed, which exhibits a rapid and sensitive color fluorescence off–on response to benzoyl peroxide (BPO) in aqueous media containing 10% ethanol via deboronation. The has been applied the simple detection of BPO real samples such as wheat flour antimicrobial agent.

Hydrogen sulfide (H2S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation H2S-derived sulfane sulfur species/polysulfides. Because high reactivity species, detection polysulfides in systems challenging currently used methods are neither sensitive nor quantitative. Herein, we describe LC-MS/MS-based method makes use Sulfane Sulfur Probe 4 to...

Sulfur dioxide (SO2) has long been considered a toxic environmental pollutant and byproduct of industrial processing. Recently it become evident that SO2 may also have regulatory functions in mammalian pulmonary systems. However, the study these effects proven to be challenging due difficulty administering reliable manner. In this work, we report discovery new pH-dependent water-soluble donor, benzothiazole sulfinate (BTS). We found BTS slow sustained release at physiological pH....

Here we report a unique reaction between phenyl diselenide-ester substrates and H2S to form 1,2-benzothiaselenol-3-one. This proceeded rapidly under mild conditions. Thiols could also react with the diselenide substrates. However, resulted S–Se intermediate retained high reactivity toward eventually led same cyclized product Based on this two fluorescent probes were developed showed selectivity sensitivity for H2S. The presence of thiols was found not interfere detection process.

Thionitrous acid (HSNO), the smallest S-nitrosothiol, is emerging as a potential key intermediate in cellular redox regulation linking two signaling molecules H2 S and NO. However, chemical biology of HSNO remains poorly understood. A major hurdle lack methods for selective detection biological systems. Herein, we report rational design, synthesis, evaluation first fluorescent probe TAP-1 detection. showed high selectivity sensitivity to aqueous media cells, providing useful tool...

Abstract Hydrogen sulfide (H 2 S) is an important signaling molecule whose up‐ and down‐regulation have specific biological consequences. Although significant advances in H S up‐regulation, by the development of donors, been achieved recent years, precise still challenging. The lack potent/specific inhibitors for S‐producing enzymes contributes to this problem. We expect scavengers alternative approach address Since chemical sensors share same criteria, we constructed a sensor database,...