A5085962935- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Plant and animal studies
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Plant and Fungal Species Descriptions
- Plant responses to water stress
- Bryophyte Studies and Records
- Entomopathogenic Microorganisms in Pest Control
- Plant Taxonomy and Phylogenetics
- Ecology and Conservation Studies
- Plant Genetic and Mutation Studies
- Cellular transport and secretion
- Light effects on plants
- Plant Pathogens and Fungal Diseases
- Insect and Arachnid Ecology and Behavior
- Plant Physiology and Cultivation Studies
Kumamoto University
2016-2025
Kumamoto Industrial Research Institute
2023-2024
Kyushu University
2024
Graduate School USA
2009-2023
The University of Tokyo
2002-2014
Collaborative Research Group
2012-2014
Tokyo University of Science
2004-2010
RIKEN
2003-2006
Nagoya University
2006
Osaka University
2006
The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical physiological adaptations to land, a life cycle with alternation between multicellular haploid diploid generations that facilitated efficient dispersal desiccation tolerant spores, in plant. We analyzed genome liverwort Marchantia polymorpha, member basal plant...
In plants and animals, small peptide ligands that signal in cell-cell communication have been suggested to be a crucial component of development. A bioassay single-cell transdifferentation demonstrates dodecapeptide with two hydroxyproline residues is the functional product genes from CLE family, which includes CLAVATA3 Arabidopsis. The suppresses xylem cell development at concentration 10(-11) M promotes division. An application, corresponding all 26 Arabidopsis protein family members,...
Land plants evolved a long-distance transport system of water and nutrients composed the xylem phloem, both which are generated from procambium- cambium-comprising vascular stem cells. However, little is known about molecular mechanism cell communication governing xylem-phloem patterning. Here, we show that dodecapeptide (HEVHypSGHypNPISN; Hyp, 4-hydroxyproline), TDIF (tracheary element differentiation inhibitory factor), secreted phloem suppresses cells into through leucine-rich repeat...
Abstract Although numerous physiological studies have addressed the interactions between brassinosteroids and auxins, little is known about underlying molecular mechanisms. Using an Affymetrix GeneChip representing approximately 8,300 Arabidopsis genes, we studied comprehensive transcript profiles over 24 h in response to indole-3-acetic acid (IAA) brassinolide (BL). We identified 409 genes as BL inducible, 276 IAA 637 total. These two hormones regulated only 48 common, suggesting that most...
The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell-specific protein presumed to be precursor of secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied in situ tissues determined the structure modified 12-amino acid (MCLV3), which was derived from conserved motif CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying typical phenotype...
Distinctive from that of the animal system, basic plan plant body is continuous formation a structural unit, composed stem with meristem at top and lateral organs continuously forming meristem. Therefore, mechanisms controlling formation, maintenance, development will be key to understanding higher plants. Genetic analyses filamentous flower (fil) mutants have indicated FIL required for maintenance growth inflorescence floral meristems, Arabidopsis thaliana. encodes protein carrying zinc...
The shoot apical meristem (SAM) is the fundamental structure that located at growing tip and gives rise to all aerial parts of plant tissues organs, such as leaves, stems flowers. In Arabidopsis thaliana, CLAVATA3 (CLV3) pathway regulates stem cell pool in SAM, which a small peptide ligand derived from CLV3 perceived by two major receptor complexes, CLV1 CLV2-CORYNE (CRN)/SUPPRESSOR OF LLP1 2 (SOL2), restrict WUSCHEL (WUS) expression. this study, we used functional, synthetic (MCLV3) isolate...
Significance Morphological adjustment is an important strategy for survival of living organisms in challenging environments. Plasticity the root system architecture critical nutrient acquisition plants. Among essential elements, nitrogen (N) strongly affects development. This article uncovers a key signaling mechanism regulating outgrowth lateral roots and expansion plant systems. The demonstrated this study suggests morphological N-poor
Despite numerous physiological studies addressing the interactions between brassinosteroids (BRs) and auxins, little is known about underlying molecular mechanisms. We studied expression of IAA5 IAA19 in response to treatment with indole acetic acid (IAA) or brassinolide (BL), most active BR. Exogenous IAA induced these genes quickly transiently, whereas exogenous BL them gradually continuously. also found that a fusion DR5, synthetic auxin element, GUS (beta-glucuronidase) gene was similar...
Summary The plant hormone, auxin, regulates many aspects of growth and development. Despite its importance, the molecular mechanisms underlying action auxin are largely unknown. To gain a more comprehensive understanding primary responses to we analyzed expression genes in Arabidopsis seedlings treated with indole‐3‐acetic acid (IAA) for 15 min. We identified single gene that is downregulated early, 29 upregulated early. Several types typical transcription factors as early genes, suggesting...
Plant roots have important roles not only in absorption of water and nutrients, but also stress tolerance such as desiccation, salt, low temperature. We investigated stress-response proteins from rice using 2-dimensional polyacrylamide-gel electrophoresis found a protein, RO-292, which was induced specifically when 2-week-old seedlings were subjected to salt drought stress. The full-length RO-292 cDNA cloned, determined encode protein 160 amino acid residues (16.9 kDa, pI 4.74). deduced...
Using 26 chemically synthetic CLAVATA3/ESR (CLE) peptides, which correspond to the predicted products of 31 Arabidopsis CLE genes, we investigated peptide function in and rice. Treatment with some peptides inhibited root elongation rice as well Arabidopsis. It also reduced size shoot apical meristem but not Database searches revealed 47 putative genes genome multiple domains indicating diverse these plants.
Arabidopsis sol2 mutants showed CLV3 peptide resistance. Twenty-six synthetic CLE peptides were examined in the clv1, clv2 and mutants. different levels of resistance to various peptides, spectrum was quite similar that clv2. SOL2 encoded a receptor-like kinase protein which is identical CORYNE (CRN). GeneChip analysis revealed expression several genes altered root tip. Here, we suggest SOL2, together with CLV2, plays an important role regulation meristem development through signaling pathway.
In Arabidopsis, the CLAVATA (CLV) pathway operates in regulation of size stem cell population shoot apical meristem (SAM). CLV3 functions as a small peptide ligand to negatively regulate expression WUSCHEL (WUS) transcription factor through three major receptor kinase complexes CLV1, CLV2-SUPPRESSOR OF LLP1-2 (SOL2)/CORYNE (CRN) and recently identified RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2)/TOADSTOOL (TOAD2). Aiming understand precise molecular details signaling, we investigated contribution...
Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ESR (CLE)-like effector proteins. These proteins have been shown to act as ligand mimics of plant CLE peptides and are required for successful nematode infection; however, the receptors CLE-like not identified. Here we demonstrate that CLV2 CORYNE (CRN), members receptor kinase family, signaling. Exogenous peptide assays overexpression CLEs in Arabidopsis demonstrated CRN perception CLEs. In addition, promoter-reporter showed both...
Over-reduction of the photosynthetic electron transport (PET) chain should be avoided, because accumulation reducing carriers produces reactive oxygen species (ROS) within photosystem I (PSI) in thylakoid membranes and causes oxidative damage to chloroplasts. To prevent production ROS H+ gradient (ΔpH) needs built up across suppress over-reduction state PET chain. In this study, we aimed identify critical component that stimulates ΔpH formation under illumination higher plants. do this,...
Abstract CLE peptides have been implicated in various developmental processes of plants and mediate their responses to environmental stimuli. However, the biological relevance most genes remains be functionally characterized. Here, we report that CLE9 , which is expressed stomata, acts as an essential regulator induction stomatal closure. Exogenous application or overexpression effectively led closure enhanced drought tolerance, whereas loss‐of‐function mutants were sensitivity stress....
Clearing techniques eliminate factors that interfere with microscopic observation, including light scattering and absorption by pigments cytoplasmic components. The allow fluorescence-based detailed analyses of materials characterization the three-dimensional structure organs. We describe a simple rapid clearing imaging method, termed 'TOMEI' (Transparent plant Organ MEthod for Imaging), which enables observation intact This method involves reagent containing 2,2′-thiodiethanol....
Phenotypic analysis of single and multiple mutants as well in situ localization the expression patterns floral genes have revealed that FIL AMENTOUS FLOWER (FIL) gene plays important roles establishing inflorescence Arabidopsis. As previously reported, fil mutant generates clusters both filamentous structures flowers with organs altered number shape. The structural resemblance to peduncles pattern APETALA1 (AP1) shown these are underdeveloped fail form receptacles organs, indicating one is...
Within the leaf of an angiosperm, vascular system is constructed in a complex network pattern called venation. The formation this vein has been widely studied as paradigm tissue plants. To elucidate molecular mechanism controlling patterning process, we previously isolated Arabidopsis mutants van1 to van7, which show discontinuous pattern. Here report phenotypic analysis van3 mutant relation auxin signaling and polar transport, characterization VAN3 gene protein. Double analyses with pin1,...