A5025271632- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Legume Nitrogen Fixing Symbiosis
- RNA and protein synthesis mechanisms
- Plant Physiology and Cultivation Studies
- Advanced Fluorescence Microscopy Techniques
- Light effects on plants
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Polysaccharides and Plant Cell Walls
- Phytase and its Applications
- Gut microbiota and health
- Nematode management and characterization studies
- Plant Parasitism and Resistance
- Biochemical and Structural Characterization
- Pancreatic and Hepatic Oncology Research
- Plant responses to water stress
- Cancer Cells and Metastasis
- bioluminescence and chemiluminescence research
- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Spectroscopy Techniques in Biomedical and Chemical Research
University of Münster
2025
University of Regensburg
2014-2023
Cluster of Excellence on Plant Sciences
2015-2016
Heinrich Heine University Düsseldorf
2008-2016
Abstract Stem cells in shoot and floral meristems of Arabidopsis thaliana secrete the signaling peptide CLAVATA3 (CLV3) that restricts stem cell proliferation promotes differentiation. The CLV3 pathway is proposed to comprise receptor kinase CLV1 receptor-like protein CLV2. We show here novel CORYNE (CRN) CLV2 act together, parallel with CLV1, perceive signal. Mutations CRN cause proliferation, similar clv1, clv2, clv3 mutants. has additional functions during plant development, including...
Stem cell number in shoot and floral meristems of Arabidopsis (Arabidopsis thaliana) is regulated by the CLAVATA3 (CLV3) signaling pathway. Perception CLV3 peptide requires receptor kinase CLV1, receptor-like protein CLV2, CORYNE (CRN). Genetic analysis suggested that CLV2 CRN act together parallel with CLV1. We studied intracellular localization fusions fluorescent tags their capacities for interaction via efficiency fluorescence resonance energy transfer. found require each other export...
Fertilization of an egg by multiple sperm (polyspermy) leads to lethal genome imbalance and chromosome segregation defects. In
Cell-cell communication and interaction is critical during fertilization triggers free cytosolic calcium ([Ca2+]cyto) as a key signal for egg activation polyspermy block in animal oocytes. Fertilization flowering plants more complex, involving of pollen tube with adjoining synergid cells, culminating release two sperm cells their fusion the central cell, respectively. Here, we report occurrence role [Ca2+]cyto signals entire double process Arabidopsis. oscillations are initiated after...
Genetic studies have identified the membrane protein EIN2 (ethylene insensitive 2) as a central component of ethylene signalling in Arabidopsis. In addition, might take part multiple hormone pathways and response to pathogens demonstrated by recent genetic biochemical studies. Here we show, an integrated approach using vivo vitro fluorescence techniques, that is localized at ER (endoplasmic reticulum) where it shows specific interaction with receptor ETR1.
Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ESR (CLE)-like effector proteins. These proteins have been shown to act as ligand mimics of plant CLE peptides and are required for successful nematode infection; however, the receptors CLE-like not identified. Here we demonstrate that CLV2 CORYNE (CRN), members receptor kinase family, signaling. Exogenous peptide assays overexpression CLEs in Arabidopsis demonstrated CRN perception CLEs. In addition, promoter-reporter showed both...
In plants, the flagellin and CLAVATA3 signaling pathways act through induced preassembled receptor complexes, respectively.
Stem cell maintenance in plants depends on the activity of small secreted signaling peptides CLAVATA3/EMBRYO SURROUNDING REGION (CLE) family, which, shoot, act through at least three kinds receptor complexes, CLAVATA1 (CLV1) homomers, CLAVATA2 (CLV2) / CORYNE (CRN) heteromers, and CLV1/CLV2/CRN multimers. In root, CLV2/CRN complexes function proximal meristem to transmit signals from CLE peptide CLE40. While CLV1 consists an extracellular domain intracellular kinase domain, CLV2, a...
mRNA translation is tightly regulated by various classes of RNA-binding proteins (RBPs) during development and in response to changing environmental conditions. In this study, we characterize the arginine-glycine-glycine (RGG) motif containing RBP family Arabidopsis thaliana representing homologues multifunctional regulators ribosomal preservation factors Stm1 from yeast (ScStm1) human SERBP1 (HsSERBP1). The genome encodes three RGG named AtRGGA, AtRGGB AtRGGC. While AtRGGA ubiquitously...
Summary Small RNAs, such as microRNAs (miRNAs), regulate gene expression and play important roles in many plant processes. Although our knowledge of their biogenesis mode action has significantly progressed, we still have comparatively little information about biological functions. In particular, spatio‐temporal patterns rely on either indirect detection by use reporter constructs or labor‐intensive direct situ hybridization sectioned material. None the current approaches allows a systematic...
Transcription of the ribosomal RNA precursor by polymerase (Pol) I is a major determinant cellular growth, and dysregulation observed in many cancer types. Here, we present purification human Pol from cells carrying genomic GFP fusion on largest subunit allowing structural functional analysis enzyme across species. In contrast to yeast, carries single-subunit stalk, vitro transcription indicates reduced proofreading activity. Determination cryo-EM reconstruction close-to-native state...
RNA binding proteins (RBPs) have multiple and essential roles in transcriptional posttranscriptional regulation of gene expression all living organisms. Their biochemical identification the proteome a given cell or tissue requires significant protein amounts, which limits studies rare highly specialized cells. As consequence, we know almost nothing about role(s) RBPs reproductive processes such as egg development, fertilization early embryogenesis flowering plants. To systematically identify...