Stem cell number in shoot and floral meristems of Arabidopsis (Arabidopsis thaliana) is regulated by the CLAVATA3 (CLV3) signaling pathway. Perception CLV3 peptide requires receptor kinase CLV1, receptor-like protein CLV2, CORYNE (CRN). Genetic analysis suggested that CLV2 CRN act together parallel with CLV1. We studied intracellular localization fusions fluorescent tags their capacities for interaction via efficiency fluorescence resonance energy transfer. found require each other export...

Article18 February 2020Open Access Transparent process Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner Arun Kumar Kondadi orcid.org/0000-0002-5888-7834 Institute Biochemistry and Molecular Biology I, Medical Faculty, Heinrich Heine University Düsseldorf, Germany Search for more papers by this author Ruchika Anand orcid.org/0000-0001-7337-6007 Sebastian Hänsch Faculty Mathematics Natural Sciences, Center Advanced Imaging, Jennifer Urbach...

PML nuclear bodies (NBs) are involved in the regulation of key pathways but their biochemical function metabolism is unknown. In this study NB assembly dynamics were assessed by live cell imaging and mathematic modeling its major component parts. We show that all six isoforms exhibit individual exchange rates at NBs identify V as a scaffold subunit. SP100 exchanges least five times faster than proteins. Turnover modulated SUMOylation. Exchange not temperature-dependent depletion cellular ATP...

To investigate the dynamics of centromere organization, we have assessed exchange rates inner proteins (CENPs) by quantitative microscopy throughout cell cycle in human cells. CENP-A and CENP-I are stable components that incorporated into centromeres via a “loading-only” mechanism G1 S phase, respectively. A subfraction CENP-H also stays stably bound to centromeres. In contrast, CENP-B, CENP-C, some hMis12 exhibit distinct cycle–specific binding stabilities, with residence times ranging from...

GBPs are essential for immunity against intracellular pathogens, especially Toxoplasma gondii control. Here, the molecular interactions of murine (mGBP1/2/3/5/6), homo- and hetero-multimerization properties mGBP2 its function in parasite killing were investigated by mutational, Multiparameter Fluorescence Image Spectroscopy, live cell microscopy methodologies. Control T. replication requires GTP hydrolysis isoprenylation thus, enabling reversible oligomerization vesicle-like structures....

Significance Synucleinopathies such as Parkinson’s disease feature deposition of misfolded α-synuclein. It is likely that cellular proteostasis compensates for α-synuclein to some extent, but, once exhausted, can form seeds a prion-like spread in the brain. Here, we demonstrate that, human dopaminergic neurons and mouse brain, H1N1 influenza virus induces aggregation by blocking protein degradation pathways. Following intranasal instillation, spreading along olfactory route into brain areas...

In plants, the flagellin and CLAVATA3 signaling pathways act through induced preassembled receptor complexes, respectively.

Article4 April 2022Open Access Source DataTransparent process PLETHORA-WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance Rebecca C Burkart orcid.org/0000-0003-0015-5312 Institute for Developmental Genetics, Heinrich-Heine University, Düsseldorf, Germany Contribution: Conceptualization, Data curation, Validation, ​Investigation, Visualization, Methodology, Writing - original draft Search more papers by this author Vivien I Strotmann...

The MBW (for R2R3MYB, basic helix-loop-helix [bHLH], and WD40) genes comprise an evolutionarily conserved gene cassette that regulates several traits such as (pro)anthocyanin anthocyanin biosynthesis epidermal cell differentiation in plants. Trichome Arabidopsis (Arabidopsis thaliana) is governed by GLABRA1 (GL1; R2R3MYB), GL3 (bHLH), transparent TESTA (TTG1; WD40). They are thought to form a trimeric complex acts transcriptional activation complex. We provide evidence these three proteins...

ABSTRACT Septins are conserved cytoskeletal structures functioning in a variety of biological processes including cytokinesis and cell polarity. A wealth information exists on the heterooligomeric architecture septins their subcellular localization at distinct sites. However, precise mechanisms assembly intracellular transport unknown. Here, we demonstrate that endosomal along microtubules is crucial for formation higher-order fungus Ustilago maydis. Importantly, septin dependent each...

The formation of plaques represents the hallmark phage infection visualizing clearance bacterial lawn in structured environments. In this study, we have addressed impact cellular development on Streptomyces undergoing a complex developmental life cycle. Analysis plaque dynamics revealed, after period size enlargement, significant regrowth transiently phage-resistant mycelium into lysis zone. venezuelae mutant strains defective at different stages indicated that was dependent onset aerial...

Abstract Bioimage data are generated in diverse research fields throughout the life and biomedical sciences. Its potential for advancing scientific progress via modern, data‐driven discovery approaches reaches beyond disciplinary borders. To fully exploit this potential, it is necessary to make bioimaging data, general, multidimensional microscopy images image series, FAIR, that is, findable, accessible, interoperable reusable. These FAIR principles management now widely accepted community...

Guanylate binding proteins (GBPs) are large interferon-inducible GTPases, executing essential host defense activities against Toxoplasma gondii , an invasive intracellular apicomplexan protozoan parasite of global importance. T . establishes a parasitophorous vacuole (PV) which shields the from host’s mechanisms. Murine GBPs (mGBPs) recognize PVs and assemble into supramolecular mGBP homo- heterocomplexes that required for disruption membrane eventually resulting in cell-autonomous immune...

Abstract Förster Resonance Energy Transfer combined with Fluorescence Lifetime Imaging Microscopy (FRET-FLIM) is revolutionizing plant biology, by enabling the study of protein–protein interactions (PPIs) within live cells. This manuscript describes principles FRET and practical application acceptor photobleaching (FRET-APB) in exploring PPIs vivo . It mainly focuses on superior characteristics FRET-FLIM details materials methods for implementing this technique plants. provides a profound...

RTX toxins, characterized by their calcium-binding glycine-rich repeat regions, are known for ability to disrupt host cell membranes. Although the acylation of specific lysine residues in these toxins is established as crucial hemolytic activity, precise mechanisms underlying this enhancement remain unclear. In study, we explore role pore-forming behaviour toxin MbxA comparing lytic activities acylated and its non-acylated form, proMbxA, on vitro membrane systems, well living cells. Our...

One of the most abundantly IFN-γ-induced protein families in different cell types is 65-kDa guanylate-binding family that recruited to parasitophorous vacuole intracellular parasite Toxoplasma gondii. Here, we elucidate relationship between biochemistry and cellular host defense functions mGBP2 response The wild type exhibits low affinities guanine nucleotides, self-assembles upon GTP binding, forming tetramers activated state, stimulates GTPase activity a cooperative manner. products two...

Autoantibodies against second extracellular loops of β1-adrenergic receptors frequent in dilated cardiomyopathy confer myocardial dysfunction presumably via cAMP stimulation. Here, we investigate the autoantibody impact on receptor conformation and function. IgG was prepared from patients with cardiomyopathy, matched healthy donors (10 each) or commercial preparations (2). binding to peptides detected 5 10 2 controls. colocalization native 8 1 controls 7 at >30 mg IgG/L). All IgGs exhibiting...

Sex in honeybees, Apis mellifera, is genetically determined by heterozygous versus homo/hemizygous genotypes involving numerous alleles at the single complementary sex determination locus. The molecular mechanism of however unknown because there are more than 4950 known possible allele combinations, but only two sexes species. We show how protein variants expressed from determiner (csd) gene determine sex. In females, amino acid differences between Csd potential-specifying domain (PSD)...

Abstract Type 1 secretion systems (T1SS) of Gram-negative bacteria are responsible for the various proteases, lipases, S-layer proteins or toxins into extracellular space. The paradigm these is hemolysin A (HlyA) T1SS Escherichia coli . This multiple membrane protein complex able to secrete toxin HlyA in one step across both E. membranes. Common all secreted substrates a C-terminal sequence being necessary as well sufficient secretion. However, it not known whether transport occurs...