A5074429774- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- RNA regulation and disease
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- Gene expression and cancer classification
- Evolution and Genetic Dynamics
- Plant Virus Research Studies
- Demographic Trends and Gender Preferences
- thermodynamics and calorimetric analyses
- Hemiptera Insect Studies
- Viral Infectious Diseases and Gene Expression in Insects
Heinrich Heine University Düsseldorf
2008-2023
Martin Luther University Halle-Wittenberg
2015
The shift from solitary to social behavior is one of the major evolutionary transitions. Primitively eusocial bumblebees are uniquely placed illuminate evolution highly insect societies. Bumblebees also invaluable natural and agricultural pollinators, there widespread concern over recent population declines in some species. High-quality genomic data will inform key aspects bumblebee biology, including susceptibility implicated viability threats.We report high quality draft genome sequences...
Organisms have evolved a bewildering diversity of mechanisms to generate the two sexes. The honeybee (Apis mellifera) employs an interesting system in which sex is determined by heterozygosity at single locus (the Sex Determination Locus) harbouring complementary determiner (csd) gene. Bees heterozygous Locus are females, whereas bees homozygous or hemizygous males. Little known, however, about regulation that links determination sexual differentiation. To investigate control development...
Effective splice site selection is critically controlled by flanking splicing regulatory elements (SREs) that can enhance or repress use. Although several computational algorithms currently identify a multitude of potential SRE motifs, their predictive power with respect to mutation effects limited. Following RESCUE-type approach, we defined hexamer-based ‘HEXplorer score’ as average Z-score all six hexamers overlapping given nucleotide in an arbitrary genomic sequence. Plotted along...
Highly social insects are characterized by caste dimorphism, with distinct size differences of reproductive organs between fertile queens and the more or less sterile workers. An abundance nutrition instruction via diet-specific compounds has been proposed as explanations for nutrition-driven queen worker polyphenism. Here, we further explored these models in honeybee (Apis mellifera) using rearing a novel mutational screening approach clustered regularly interspaced short palindromic...
Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack tools that allow measuring neuronal activity simultaneously different brain regions. Here, we developed first pan-neuronal genetic driver Hymenopteran model organism, honey bee, and expressed calcium indicator GCaMP6f under control bee synapsin promoter. We show is widely brain, allowing to record from multiple To assess power this tool, focused on olfactory system, recording...
Abstract Background The HIV-1 Rev protein is a key component in the early to late switch splicing from intronless (e.g. tat , rev ) intron-containing Rev-dependent gag vif env transcripts. Previous results suggested that cis -acting sequences and inefficient 5' 3' splice sites are prerequisite for function. However, we other groups have shown two of sites, D1 D4, efficiently used vitro vivo . Here, focus on efficiency taking into consideration what extent their intrinsic efficiencies...
Sex in honeybees, Apis mellifera, is genetically determined by heterozygous versus homo/hemizygous genotypes involving numerous alleles at the single complementary sex determination locus. The molecular mechanism of however unknown because there are more than 4950 known possible allele combinations, but only two sexes species. We show how protein variants expressed from determiner (csd) gene determine sex. In females, amino acid differences between Csd potential-specifying domain (PSD)...
Identifying the forces shaping intraspecific phenotypic and genotypic divergence are of key importance in evolutionary biology. Phenotypic may result from local adaptation or, especially species with strong gene flow, pronounced plasticity. Here, we examine morphological genetic among populations western honey bee Apis mellifera topographically heterogeneous East African region. The currently accepted "mountain refugia hypothesis" states that living disjunct montane forests belong to a...
ABSTRACT Small noncoding HIV-1 leader exon 3 is defined by its splice sites A2 and D3. While 3′ site (3′ss) needs to be activated for vpr mRNA formation, the location of start codon within downstream intron requires silencing splicing at 5′ss Here we show that inclusion both processing promoted an exonic enhancer (ESE ) localized between silencer ESSV The ESE sequence was found bound members Transformer 2 (Tra2) protein family. Coexpression these proteins in provirus-transfected cells led...
Functional genetic studies in honeybees have been limited by transformation tools that lead to a high rate of transposon integration into the germline queens. A is required reduce screening efforts because each treated queen needs be maintained separate honeybee colony. Here, we report on further improvement using combination different procedures. We employed hyperactive transposase protein (hyPBase
Hygienic behavior (HB) enables honeybees to tolerate parasites, including infection with the parasitic mite Varroa destructor, and it is a well-known example of quantitative genetic trait. The understanding molecular processes underpinning differences in this remains limited. We performed gene expression studies worker bees that displayed HB. established high low source HB performance studied engagements into single under same environmental conditions. found percentage engaged hygienic...
Abstract The honey bee, A pis mellifera , displays a rich behavioural repertoire, social organization and caste differentiation, has an interesting mode of sex determination, but we still know little about its underlying genetic programs. We lack stable transgenic tools in bees that would allow control gene activity lines. As initial step towards method, identified promoter sequences the bee can drive constitutive, tissue‐specific cold shock‐induced expression. m‐actin5c elp2l m‐hsp83...
Short linear motifs (SLiMs) can play pivotal functional roles in proteins, such as targeting proteins to specific subcellular localizations, modulating the efficiency of translation and tagging for degradation. Until recently we had little knowledge about SLiM evolution. Only a few amino acids these are functionally important, making them likely evolve ex nihilo suggesting that they key protein Several reports now suggest appear disappear while their function is preserved, process sometimes...
ABSTRACT Understanding of the neural bases for complex behaviors in Hymenoptera insect species has been limited by a lack tools that allow measuring neuronal activity simultaneously different brain regions. Here, we developed first pan-neuronal genetic driver Hymenopteran model organism, honey bee, and expressed calcium indicator GCaMP6f under control bee synapsin promoter. We show is widely brain, allowing to record from multiple To assess power this tool, focused on olfactory system,...
Effective selection between true and decoy splice sites is critically controlled by flanking splicing regulatory elements (SREs), which can enhance or repress site use. Recent experimental evidence suggests that the entire regional context of SREs rather than a single enhancer/silencer hexamer jointly contribute to splicing. Extending score concept [Fairbrother et al.: Science 2002, 297:1007-13], we represent property an 5’ss neighborhood weighted average normalized Z-scores for all...