A5071566036- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Insect-Plant Interactions and Control
- Science and Education Research
- Insect Resistance and Genetics
- RNA modifications and cancer
- Environmental Sustainability and Education
- Genetics, Bioinformatics, and Biomedical Research
- Insect symbiosis and bacterial influences
- Education Pedagogy and Practices
- Mechanical stress and fatigue analysis
- Evolution and Genetic Dynamics
- Rural and Ethnic Education
- Health, Education, and Aging
- Insect Utilization and Effects
- Malaria Research and Control
- Parasites and Host Interactions
- Molecular Biology Techniques and Applications
- Banana Cultivation and Research
- Gene expression and cancer classification
- Fuel Cells and Related Materials
- HVDC Systems and Fault Protection
- Studies on Chitinases and Chitosanases
Universidade de São Paulo
2008-2025
Universidade Federal de São Carlos
2013-2022
Universidade de Ribeirão Preto
2006-2022
Universidade Estadual Paulista (Unesp)
2008
Universidade Federal de Uberlândia
2007
Universidade Brasil
2006
The shift from solitary to social behavior is one of the major evolutionary transitions. Primitively eusocial bumblebees are uniquely placed illuminate evolution highly insect societies. Bumblebees also invaluable natural and agricultural pollinators, there widespread concern over recent population declines in some species. High-quality genomic data will inform key aspects bumblebee biology, including susceptibility implicated viability threats.We report high quality draft genome sequences...
For bees, many roads lead to social harmony Eusociality, where workers sacrifice their reproductive rights support the colony, has evolved repeatedly and represents most form of evolution in insects. Kapheim et al. looked across genomes 10 bee species with varying degrees sociality determine underlying genomic contributions. No one path led eusociality, but similarities were seen features such as increases gene regulation methylation. It also seems that selection pressures relaxed after...
Abstract Background Hexamerins are hemocyanin-derived proteins that have lost the ability to bind copper ions and transport oxygen; instead, they became storage proteins. The current study aimed broaden our knowledge on hexamerin genes found in honey bee genome by exploring their structural characteristics, expression profiles, evolution, functions life cycle of workers, drones queens. Results ( hex 70a , 70b 70c 110 ) diverge considerably structure, so overall amino acid identity shared...
RNA interference has been frequently applied to modulate gene function in organisms where the production and maintenance of mutants is challenging, as our model study, honey bee, Apis mellifera. A green fluorescent protein (GFP)-derived double-stranded (dsRNA-GFP) currently commonly used control bee RNAi experiments, since its does not exist A. mellifera genome. Although dsRNA-GFP expected trigger responses treated bees, undesirable effects on expression, pigmentation or developmental timing...
Major developmental transitions in multicellular organisms are driven by steroid hormones. In insects, these, together with juvenile hormone (JH), control development, metamorphosis, reproduction and aging, also suggested to play an important role caste differentiation of social insects. Here, we aimed determine how EcR transcription ecdysteroid titers related during honeybee postembryonic development what may actually be the this insect. addition, expected that knocking-down gene expression...
Abstract The honey bee queen and worker castes are a model system for developmental plasticity. We used established expressed sequence tag information Gene Ontology based annotation of genes that differentially during caste development. Metabolic regulation emerged as major theme, with caste‐specific difference in the expression oxidoreductases vs. hydrolases. Motif searches upstream regions revealed group‐specific motifs, providing an entry point to cis ‐regulatory network studies on genes....
Summary In honey bees, Vitellogenin (Vg) is hypothesized to be a major factor affecting hormone signaling, food-related behavior, immunity, stress resistance and lifespan. Likewise microRNAs play important roles in posttranscriptional gene regulation affect many biological processes. The action of Vg are known intersect the context reproduction; however, role these associations on social behavior unknown. phenotypic effects knockdown best established studied forager stage workers. Thus, we...
Abstract Queen and worker honeybees differ profoundly in reproductive capacity. The queen of this complex society, with 200 highly active ovarioles each ovary, is the fertile caste, whereas workers have approximately 20 as a result receiving different diet during larval development. In regular queenright colony, inactive ovaries do not reproduce. However, if sensed to be absent, some activate their ovaries, producing viable haploid eggs that develop into males. Here, deep‐sequenced ovary...
Abstract Stingless bees are generalist pollinators distributed through the pantropical region. There is growing evidence that their wild populations experiencing substantial decline in response to habitat degradation and pesticides. Policies for conservation of endangered species will benefit from studies focusing on genetic molecular aspects development behavior. The most common method looking at gene expression real-time quantitative polymerase chain reaction preceded by reverse...
Ftz-f1 is an orphan member of the nuclear hormone receptor superfamily. A 20-hydroxyecdysone pulse allows ftz-f1 gene expression, which then regulates activity downstream genes involved in major developmental progression events. In honeybees, expression like vitellogenin (vg), prophenoloxidase and juvenile hormone-esterase during late pharate-adult development known to be hormonally controlled both queens workers by increasing (JH) titres presence declining levels ecdysteroids. Since for...
Abstract Background Most of our understanding on the social behavior and genomics bees other insects is centered Western honey bee, Apis mellifera. The genus Apis, however, a highly derived branch comprising less than dozen species, four which genomically characterized. In contrast, for equally eusocial, yet taxonomically biologically more diverse Meliponini, full genome sequence was so far available single Melipona species only. We present here Frieseomelitta varia , stingless bee that has,...
Abstract Insect development is primarily controlled by juvenile hormone (JH) and 20-hydroxyecdysone (20E), which regulate gene cascades leading to changes in phenotype, physiology, behavior. Besides these hormones, microRNAs play a crucial role insect regulating expression at the post-transcriptional level. To advance molecular understanding of holometabolous developmental events, we investigate pupal phase honeybee, Apis mellifera . In this study, assessed profiles genes components JH 20E –...
Abstract An N‐terminal amino acid sequence of a previously reported honey bee hexamerin, HEX 110 [Danty et al., Insect Biochem Mol Biol 28:387–397 (1998)], was used as reference to identify the predicted genomic in public GenBank database. In silico analysis revealed an ORF 3,033 nucleotides that encompasses eight exons. The conceptual translation product is glutamine‐rich polypeptide with molecular mass 112.2 kDa and p I 6.43, which contains conserved M C hemocyanin domains....
Cuticle renewal is a complex biological process that depends on the cross talk between hormone levels and gene expression. This study characterized expression of two genes encoding cuticle proteins sharing four conserved amino acid blocks Tweedle family, AmelTwdl1 AmelTwdl2, peroxidase containing Animal haem domain, Ampxd, in honey bee. Gene sequencing annotation validated formerly predicted tweedle genes, revealed novel gene, bee genome. Expression these was studied context...
Epitranscriptomics is an emerging field of investigation dedicated to the study post-transcriptional RNA modifications. methylations regulate metabolism and processing, including changes in response environmental cues. Although modifications are conserved from bacteria eukaryotes, there little evidence epitranscriptomic pathway insects. Here we identified genes related m6 A (N6-methyladenine) m5 C (5-methylcytosine) methylation machinery seven bee genomes (Apis mellifera, Melipona...
One of the commonest neotropical stingless bees is Tetragonisca angustula (Latreille, 1811), popularly known in Portuguese as jataí, which occurs variable nesting sites from Mexico to Argentina. We used 18 primers generate 218 RAPD markers we determined genetic distance between T. populations 25 localities three different Latin America countries, using weyrauchi Brazilian state Acre and common honey bee (Apis mellifera) outgroups. Genetic distance, calculated Percentage Dissimilarity (14%),...
The ongoing efforts to sequence the honey bee genome require additional initiatives define its transcriptome. Towards this end, we employed Open Reading frame ESTs (ORESTES) strategy generate profiles for life cycle of Apis mellifera workers.
Abstract Farnesoic acid O‐methyl transferase (FAMeT) is the enzyme that catalyzes formation of methyl farnesoate (MF) from farnesoic (FA) in biosynthetic pathway juvenile hormone (JH). This work reports cloning, sequencing, and expression FAMeT gene stingless bee Melipona scutellaris (MsFAMeT). The MsFAMeT silico analysis showed greatest sequence similarity found Apis mellifera other insects, while relatively less shown crustaceans. Evidence alternative splicing a 27 nucleotide (nt)...
Abstract Hexamerins and prophenoloxidases (PPOs) proteins are members of the arthropod‐haemocyanin superfamily. In contrast to haemocyanin PPO, hexamerins do not bind oxygen, but mainly play a role as storage that supply amino acids for insect metamorphosis. We identified seven genes encoding hexamerins, three PPOs, one hexamerin pseudogene in genome parasitoid wasp Nasonia vitripennis . A phylogenetic analysis PPOs from this related other orders suggests an essentially order‐specific...
Mass spectrometry is essential for large-scale protein identification in proteomics. By database searching of mass data, it possible to identify proteins without the need interpret MS/MS spectra (de novo sequencing), which time-consuming and not feasible as a systematic tool proteomic analysis. Many authors have reported initiatives teach undergraduate students, but usually emphasized. Here, we propose class about data order complement theoretical proteomics course. It includes presentation...