A5006238710- Mass Spectrometry Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Academic Writing and Publishing
- Neurobiology and Insect Physiology Research
- Metabolomics and Mass Spectrometry Studies
- Microfluidic and Bio-sensing Technologies
- Ion-surface interactions and analysis
- Receptor Mechanisms and Signaling
- Analytical Chemistry and Chromatography
- Amino Acid Enzymes and Metabolism
- Neuropeptides and Animal Physiology
- Analytical Chemistry and Sensors
- Various Chemistry Research Topics
- Neuroscience and Neuropharmacology Research
- Biosensors and Analytical Detection
- Photoreceptor and optogenetics research
- scientometrics and bibliometrics research
- Innovative Microfluidic and Catalytic Techniques Innovation
- NMR spectroscopy and applications
- Nanopore and Nanochannel Transport Studies
- Electrochemical Analysis and Applications
- Neuroscience and Neural Engineering
- Advanced biosensing and bioanalysis techniques
- Neuroendocrine regulation and behavior
University of Illinois Urbana-Champaign
2016-2025
Chan Zuckerberg Biohub Chicago
2024-2025
Society for Neuroscience
2008-2025
Chan Zuckerberg Initiative (United States)
2024-2025
Illinois College
2024
Chan Zuckerberg Biohub San Francisco
2024
Urbana University
2003-2022
University of Nebraska–Lincoln
2022
Bioengineering Center
2007-2019
University of Minnesota System
2015
The genome of the zebra finch — a songbird and model for study vertebrate brain, behaviour evolution has been sequenced. Its comparison with chicken genome, only other bird available, shows that genes neural function implicated in cognitive processing song have rapidly evolving lineage. also vocal communication engages much brain transcriptome identifies potential integrator microRNA signals linked to communication. studying Comparison are Moreover, brain. is an important organism several...
The origins of neural systems remain unresolved. In contrast to other basal metazoans, ctenophores (comb jellies) have both complex nervous and mesoderm-derived muscular systems. These holoplanktonic predators also sophisticated ciliated locomotion, behaviour distinct development. Here we present the draft genome Pleurobrachia bachei, Pacific sea gooseberry, together with ten ctenophore transcriptomes, show that they are remarkably from animal genomes in their content neurogenic, immune...
High-resolution, proton nuclear magnetic resonance (NMR) spectra of 5-nanoliter samples have been obtained with much higher mass sensitivity [signal-to-noise ratio (S/N) per micromole] than traditional methods. Arginine and sucrose show a mean enhancement 130 compared to 278-microliter run in 5-millimeter tube conventional, commercial probe. This can reduce data acquisition time by factor >16,000 or the needed sample about 130. A linewidth 0.6 hertz was achieved on 300-megahertz...
Genomic/peptidomic analyses of the planarian Schmidtea mediterranea identifies >200 neuropeptides and uncovers a conserved neuropeptide required for proper maturation maintenance reproductive system.
Daily rhythms of mammalian physiology, metabolism, and behavior parallel the day-night cycle. They are orchestrated by a central circadian clock in brain, suprachiasmatic nucleus (SCN). Transcription genes is sensitive to metabolic changes reduction oxidation (redox); however, cycles protein have been reported anucleate cells, where no transcription occurs. We investigated whether SCN also expresses redox how such oscillations might affect neuronal physiology. detected self-sustained state...
Abstract Spatial omics technologies can reveal the molecular intricacy of brain. While mass spectrometry imaging (MSI) provides spatial localization compounds, comprehensive biochemical profiling at a brain-wide scale in three dimensions by MSI with single-cell resolution has not been achieved. We demonstrate complementary and mapping using MEISTER, an integrative experimental computational (MS) framework. Our framework integrates deep-learning-based reconstruction that accelerates...
Neuropeptides, critical brain peptides that modulate animal behavior by affecting the activity of almost every neuronal circuit, are inherently difficult to predict directly from a nascent genome sequence because extensive posttranslational processing. The combination bioinformatics and proteomics allows unprecedented neuropeptide discovery an unannotated genome. Within Apis mellifera genome, we have inferred more than 200 neuropeptides confirmed sequences 100 peptides. This study lays...
The feasibility of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging features smaller than the beam size has been demonstrated. method involves complete ablation MALDI matrix coating sample at each position and moving target a distance less diameter before repeating process. In limit ablation, acquiring signal from adjacent positions spaced by distances probe enhances image resolution as measured analyte only arises overlap non-ablated surface. Image...
NeuroPred is a web application designed to predict cleavage sites at basic amino acid locations in neuropeptide precursor sequences. The user can study one sequence or multiple sequences simultaneously, selecting from several prediction models and optional, user-defined functions. Logistic regression are trained on experimentally verified published data mollusks, mammals insects, motifs reported be associated with cleavage. Confidence interval limits of the probabilities indicate precision...
4,5-Diaminofluorescein (DAF-2) is widely used for detection and imaging of NO based on its sensitivity, noncytotoxicity, specificity. In the presence oxygen, NO-related reactive nitrogen species nitrosate 4,5-diaminofluorescein to yield highly fluorescent DAF-2 triazole (DAF-2T). However, as reported here, reaction form a product not specific because it reacts with dehydroascorbic acid (DHA) ascorbic (AA) generate new compounds that have fluorescence emission profiles similar DAF-2T. When...
A method that enables metabolomic profiling of single cells and subcellular structures is described using capillary electrophoresis coupled to electrospray ionization time-of-flight mass spectrometry. nebulizer-free coaxial sheath-flow interface completes the circuit provides a stable electrospray, yielding signal with relative standard deviation under 5% for total ion electropherogram. Detection limits are in low nanomolar range (i.e., <50 nM (<300 amol)) number cell-to-cell signaling...
The extension of microfluidic devices to include three-dimensional fluidic networks allows complex and chemical manipulations but requires innovative methods interface layers. Externally controllable interconnects, employing nuclear track-etched polycarbonate membranes containing nanometer-diameter capillaries, are described that produce hybrid architectures. Controllable nanofluidic transfer is achieved by controlling applied bias, polarity, density the immobile nanopore surface charge...
Microfluidic devices have been used to study high-density cultures of many cell types. Because cell-to-cell signaling is local, however, there exists a need develop culture systems that sustain small numbers neurons and enable analyses the microenvironments. Such are hard maintain in stable form, it difficult prevent death when using primary mammalian neurons. We demonstrate postnatal hippocampal from rat can be cultured at low densities within nanoliter-volume microdevices fabricated...
N-methyl-D-aspartate receptors (NMDARs) subserve numerous neurophysiological and neuropathological processes in the cerebral cortex. Their activation requires binding of glutamate also a coagonist. Whereas glycine D-serine (D-ser) are candidates for such role at central synapses, nature coagonist cortex remains unknown. We first show that glycine-binding site NMDARs is not saturated acute slices preparations medial prefrontal (mPFC). Using enzymes selectively degrade either D-ser or glycine,...
The chemical differences between individual cells within large cellular populations provide unique information on organisms' homeostasis and the development of diseased states. Even genetically identical cell lineages diverge due to local microenvironments stochastic processes. minute sample volumes low abundance some constituents in hinder our understanding heterogeneity. Although amplification methods facilitate single-cell genomics transcriptomics, characterization metabolites proteins...
Glial cells are increasingly recognized as active players that profoundly influence neuronal synaptic transmission by specialized signaling pathways. In particular, astrocytes have been shown recently to release small molecules, such the amino acids l -glutamate and d -serine “gliotransmitters,” which directly control efficacy of adjacent synapses. However, it is still controversial whether gliotransmitters released from a cytosolic pool or Ca 2+ -dependent exocytosis secretory vesicles,...