A5032142423- Neuroscience and Neuropharmacology Research
- Amino Acid Enzymes and Metabolism
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Epigenetics and DNA Methylation
- Advanced Fluorescence Microscopy Techniques
- Diet and metabolism studies
- Tryptophan and brain disorders
- Photoreceptor and optogenetics research
- Cancer Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Molecular Sensors and Ion Detection
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- Adenosine and Purinergic Signaling
- Metabolism and Genetic Disorders
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Neurodevelopmental Disorders
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Protease and Inhibitor Mechanisms
- Ion channel regulation and function
Inserm
2006-2023
Neurocentre Magendie
2013-2023
Institut Interdisciplinaire de Neuroscience
2016-2022
Université de Bordeaux
2008-2022
Centre National de la Recherche Scientifique
2008-2021
University of Münster
2013-2015
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2005-2008
University of Insubria
2005
The gliotransmitter d -serine is released upon (S)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate and metabotropic glutamate receptor stimulation, but the mechanisms involved are unknown. Here, by using a highly sensitive bioassay to continuously monitor extracellular levels, we have investigated pathways used in its release. We reveal that release inhibited removal of calcium augmented increasing or after treatment with Ca 2+ ionophore A23187. Furthermore, amino acid...
Glial cells are increasingly recognized as active players that profoundly influence neuronal synaptic transmission by specialized signaling pathways. In particular, astrocytes have been shown recently to release small molecules, such the amino acids l -glutamate and d -serine “gliotransmitters,” which directly control efficacy of adjacent synapses. However, it is still controversial whether gliotransmitters released from a cytosolic pool or Ca 2+ -dependent exocytosis secretory vesicles,...
Human genes coding for pLG72 and d-amino acid oxidase have recently been linked to the onset of schizophrenia. was proposed as an activator human FAD-containing flavoprotein (hDAAO). In brain this oxidizes d-serine, a potent N-methyl-d-aspartate receptor. We investigated mechanistic regulation hDAAO by pLG72. Immunohistochemical analyses revealed that are both expressed in astrocytes cortex, where they most likely interact, considering their partial overlapping subcellular distribution...
Significance NMDA receptors (NMDARs) support patterning and activity of synapses throughout life are central to many brain disorders. The NMDAR activation requires the concomitant binding glutamate a coagonist glycine or d -serine. To date, whether preference for one at specific connections occurs remains unsolved. Here, we sought determine when where -serine enter into play hippocampal synapses. We demonstrate that identity is synapse developmentally regulated. Remarkably, this segregation...
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe "semisynthetic" pH-sensitive conjugates with organic fluorophores, carbofluorescein, Virginia Orange that match the properties SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization both constituting new bright sensors for these key steps synaptic transmission.
Long-term depression (LTD) of synaptic strength can take multiple forms and contribute to circuit remodeling, memory encoding or erasure. The generic term LTD encompasses various induction pathways, including activation NMDA, mGlu P2X receptors. However, the associated specific molecular mechanisms effects on physiology are still unclear. We here compare how NMDAR- P2XR-dependent affect nanoscale organization function in rodents. While both LTDs with a loss reorganization AMPARs, only...
D-Serine is an astrocyte-derived regulator for N-methyl-D-aspartate receptors, but the intracellular routes of its trafficking are still largely unknown. Here, we combined confocal microscopy with colocalization quantification to track astrocytic organelles that store D-serine. We report D-serine colocalizes transfected eGFP-synaptobrevin/VAMP2 and eGFP-cellubrevin/VAMP3, two v-SNAREs regulated secretory pathway. No significant was found markers endosomal sorting recycling system: EEA1,...
Glutamate is the major excitatory transmitter in vertebrate nervous system. To maintain synaptic efficacy, recycling vesicles (SV) are refilled with glutamate by vesicular transporters (VGLUTs). The dynamics and mechanism of uptake intact neurons still largely unknown. Here, we show live-cell imaging pH- chloride-sensitive fluorescent probes cultured hippocampal wild-type VGLUT1-deficient mice that SVs VGLUT functions as a glutamate/proton exchanger associated channel-like chloride...
Abstract The patterns of development the vestibular nuclei (VN) and their main connections involving glutamate neurotransmission offer a good model for studying function glial‐derived neuromodulator D ‐serine in synaptic plasticity. In this study we show that is present VN analyzed its distribution levels expression serine racemase ‐amino acid oxidase ( ‐AAO) at different stages postnatal (P) development. From birth to P21, high were detected glial cells processes all parts VN. This period...
In chemical synapses undergoing high frequency stimulation, vesicle components can be retrieved from the plasma membrane via a clathrin-independent process called activity-dependent bulk endocytosis (ADBE). Alix (ALG-2-interacting protein X/PDCD6IP) is an adaptor binding to ESCRT and endophilin-A proteins which required for in fibroblasts. expressed neurons concentrates at during epileptic seizures. Here, we used cultured show that recruited presynapses where it interacts with conditions...
Long-Term Potentiation (LTP) and Depression (LTD) of excitatory synaptic transmission are considered as cellular basis learning memory. These two forms plasticity have been mainly attributed to global changes in the number AMPA-type glutamate receptor (AMPAR) through a regulation diffusion/trapping balance at PSD, exocytosis endocytosis. While precise molecular mechanisms base LTP intensively investigated, ones involved LTD remains elusive. Here we combined super-resolution imaging...
Abstract The enteric nervous system (ENS) is a complex network of diverse molecularly defined classes neurons embedded in the gastrointestinal wall and responsible for controlling major functions gut. As central system, vast array ENS interconnected by chemical synapses. Despite several studies reporting expression ionotropic glutamate receptors ENS, their roles gut remain elusive. Here, using an immunohistochemistry, molecular profiling functional assays, we uncover new role D-serine...
Abstract The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe ‘semisynthetic’ pH-sensitive conjugates that match the properties SEP. Conjugation to genetically encoded self-labeling tags or antibodies allows visualization both constituting new bright sensors for these key steps synaptic transmission.