A5044717339- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Near-Field Optical Microscopy
- RNA Research and Splicing
- Force Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Cellular Mechanics and Interactions
- Orbital Angular Momentum in Optics
- Neurogenesis and neuroplasticity mechanisms
- Microfluidic and Bio-sensing Technologies
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Analytical Chemistry and Sensors
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
- Neurobiology and Insect Physiology Research
- Receptor Mechanisms and Signaling
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cellular transport and secretion
- Advanced Biosensing Techniques and Applications
Université de Bordeaux
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Institut Interdisciplinaire de Neuroscience
2014-2022
Imagine Optic (France)
2014-2022
University of Central Florida
2012
Abstract The advent of super-resolution imaging (SRI) has created a need for optimized labelling strategies. We present new method relying on fluorophore-conjugated monomeric streptavidin (mSA) to label membrane proteins carrying short, enzymatically biotinylated tag, compatible with SRI techniques including uPAINT, STED and dSTORM. demonstrate efficient specific target in confined intercellular organotypic tissues, reduced steric hindrance no crosslinking compared multivalent probes. use...
Abstract Multicolor single-molecule localization microscopy (λSMLM) is a powerful technique to reveal the relative nanoscale organization and potential colocalization between different molecular species. While several standard analysis methods exist for pixel-based images, λSMLM still lacks such standard. Moreover, existing only work on 2D data are usually sensitive organization, very important parameter consider in quantitative SMLM. Here, we present an efficient, parameter-free method 3D...
Long-term depression (LTD) of synaptic strength can take multiple forms and contribute to circuit remodeling, memory encoding or erasure. The generic term LTD encompasses various induction pathways, including activation NMDA, mGlu P2X receptors. However, the associated specific molecular mechanisms effects on physiology are still unclear. We here compare how NMDAR- P2XR-dependent affect nanoscale organization function in rodents. While both LTDs with a loss reorganization AMPARs, only...
Abstract Direct interactions between receptors at the neuronal surface have long been proposed to tune signaling cascades and communication in health disease. Yet, lack of direct investigation methods measure, live neurons, interaction different membrane single molecule level has raised unanswered questions on biophysical properties biological roles such receptor interactome. Using a multidimensional spectral molecule-localization microscopy (MS-SMLM) approach, we monitored two receptors,...
Hippocampal pyramidal neurons are characterized by a unique arborization subdivided in segregated dendritic domains receiving distinct excitatory synaptic inputs with specific properties and plasticity rules that shape their respective contributions to integration action potential firing. Although the basal regulation plastic range of proximal distal synapses known be different, composition nanoscale organization key proteins at these remains largely elusive. Here we used superresolution...
Spurious blinking fluorescent spots are often seen in bacteria during single-molecule localization microscopy experiments. Although this 'autoblinking' phenomenon is widespread, its origin remains unclear. In Deinococcus strains, we observed particularly strong autoblinking at the periphery of bacteria, facilitating comprehensive characterization. A systematic evaluation contributions different components sample environment to levels and in-depth analysis photophysical properties molecules...
Optical trapping of single biological cells has become an established technique for controlling and studying fundamental behavior with their environment without having "many-body" interference. The development such instrument optical diagnostics (including Raman fluorescence molecular diagnostics) via laser spectroscopy either the "trapping" beam or secondary beams is still in progress. This paper shows modular multi-spectral imaging tweezers combining Fluorescence cells.
Abstract Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions vertebrates. We confirm that determinants first order phase transition SAP97/hDLG controlled by morpho-functional changes its nanoscale organization. Furthermore, signatures these signalling islands and transitions are altered response to cytosolic Ca 2+ . Additionally, exchange...
Long-Term Potentiation (LTP) and Depression (LTD) of excitatory synaptic transmission are considered as cellular basis learning memory. These two forms plasticity have been mainly attributed to global changes in the number AMPA-type glutamate receptor (AMPAR) through a regulation diffusion/trapping balance at PSD, exocytosis endocytosis. While precise molecular mechanisms base LTP intensively investigated, ones involved LTD remains elusive. Here we combined super-resolution imaging...
Journal Article Exchange Dynamics of Dynamin Measured in Living Cells During Endocytic Vesicle Formation Get access Léa Claverie, Claverie University Bordeaux, Interdisciplinary Institute for Neuroscience, UMR 5297, F-33000 FranceCNRS, France Search other works by this author on: Oxford Academic Google Scholar Morgane Rosendale, Rosendale Patrice Maschalchi, Maschalchi Bordeaux Imaging Center, UMS 3420 CNRS, US4 INSERM, Corey Butler, Butler Natacha Retailleau, Retailleau Jean-Baptiste...
Abstract Transient information input to brain leads persistent changes in synaptic circuit, thereby forming memory engrams. Synapse undergoes coordinated functional and structural during this process but how such are achieved by its component molecules still largely remain enigmatic. We found that activated CaMKII, the central player of plasticity, liquid-liquid phase separation (LLPS) with NMDAR subunit GluN2B. Due CaMKII autophosphorylation, condensate stably persists even after Ca 2+ is...