A5023479832- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Neuroscience and Neural Engineering
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Genetics and Neurodevelopmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Cerebrospinal fluid and hydrocephalus
- Traumatic Brain Injury and Neurovascular Disturbances
- Barrier Structure and Function Studies
- Autism Spectrum Disorder Research
- CRISPR and Genetic Engineering
- Advanced Neuroimaging Techniques and Applications
- Central Venous Catheters and Hemodialysis
- Neuropeptides and Animal Physiology
- Cancer, Stress, Anesthesia, and Immune Response
- Molecular Communication and Nanonetworks
- Nuclear Receptors and Signaling
- Migration, Health and Trauma
- Thermal Regulation in Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- Intramuscular injections and effects
Achucarro Basque Center for Neuroscience
2017-2025
Fundación Biofísica Bizkaia
2024-2025
Research Network (United States)
2022-2024
University of the Basque Country
2017-2024
Institut Interdisciplinaire de Neuroscience
2011-2024
Université de Bordeaux
2011-2024
Consejo Superior de Investigaciones Científicas
2024
Centre National de la Recherche Scientifique
2011-2020
Lund University
1984-2013
Odense University Hospital
2009
The optogenetic approach to gain control over neuronal excitability both in vitro and vivo has emerged as a fascinating scientific tool explore networks, but it also opens possibilities for developing novel treatment strategies neurologic conditions. We have explored whether such an using the light-driven halorhodopsin chloride pump from Natronomonas pharaonis (NpHR), modified mammalian CNS expression hyperpolarize central neurons, may inhibit excessive hyperexcitability epileptiform...
Dopamine (DA) cell replacement therapy in Parkinson disease (PD) can be achieved using human fetal mesencephalic tissue; however, limited tissue availability has hindered further developments. Embryonic stem cells provide a promising alternative, but poor survival and risk of teratoma formation have prevented their clinical application. We present here method for generating large numbers DA neurons based on expanding differentiating ventral midbrain (VM) neural cells/progenitors the presence...
Neurons are perpetually receiving vast amounts of information in the form synaptic input from surrounding cells. The majority occurs at thousands dendritic spines, which mediate excitatory transmission brain, and is integrated by somatic compartments postsynaptic neuron. functional role spines shaping biochemical electrical signals transmitted via synapses has long been intensely studied. Yet, many basic questions remain unanswered, particular regarding impact their nanoscale morphology on...
Intrastriatal grafts of stem cell-derived dopamine (DA) neurons induce behavioral recovery in animal models Parkinson's disease (PD), but how they functionally integrate host neural circuitries is poorly understood. Here, Wnt5a-overexpressing cells derived from embryonic ventral mesencephalon tyrosine hydroxylase-GFP transgenic mice were expanded as neurospheres and transplanted into organotypic cultures wild type mouse striatum. Differentiated GFP-labeled DA the exhibited mature neuronal...
Dravet syndrome is a genetic encephalopathy characterized by severe epilepsy combined with motor, cognitive, and behavioral abnormalities. Current antiepileptic drugs achieve only partial control of seizures provide little benefit on the patient's neurological development. In >80% cases, disease caused haploinsufficiency SCN1A gene, which encodes alpha subunit Nav1.1 voltage-gated sodium channel. Novel therapies aim to restore expression in order address all manifestations. We evidence that...
Laser Doppler flowmetry (LDF) is a recent technique that increasingly being used to monitor relative changes in cerebral blood flow whereas the intra-arterial 133xenon injection well-established method for repeated absolute measurements of flow. The aim this study was validate LDF assessment autoregulation and CO2 reactivity with as gold standard. Simultaneous (CBF) were collected by (CBF(LDF)) (CBF(Xe)) while (1) challenged controlled systemic haemorrhage, or (2) varied manipulating...
Cl(-) plays a crucial role in neuronal function and synaptic inhibition. However, the impact of morphology on diffusion redistribution intracellular is not well understood. The spines along dendritic trees has been addressed so far. Because measuring fast spatially restricted changes within dendrites yet technically possible, we used computational approaches to predict effects dynamics morphologically complex dendrites. In all morphologies tested, including imaged by super-resolution STED...
Fluorescence microscopy remains one of the single most widely applied experimental approaches in neuroscience and beyond is continuously evolving to make it easier more versatile. The success approach based on synergistic developments imaging technologies fluorophore labeling strategies that have allowed greatly diversify be used across preparations for addressing structure as well function. Yet, while targeted are a key strength fluorescence microscopy, they reciprocally impose general...
Background: It has been consistently reported that the deficiency of adenosine triphosphate (ATP) sensitive purinergic receptor P2X7 (P2X7R) ameliorates symptoms in animal models brain diseases. Objective: This study aimed to investigate role P2X7R rodent acute and subchronic schizophrenia based on phencyclidine (PCP) delivery animals lacking or overexpressing P2X7R, identify underlying mechanisms involved. Methods: The psychotomimetic effects i.p. PCP administration C57Bl/6J wild-type,...