A5073624818- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Force Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- Advanced Electron Microscopy Techniques and Applications
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- Skin and Cellular Biology Research
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Advanced biosensing and bioanalysis techniques
- Caveolin-1 and cellular processes
- Biocrusts and Microbial Ecology
- Cardiomyopathy and Myosin Studies
- Spaceflight effects on biology
- Gold and Silver Nanoparticles Synthesis and Applications
- Muscle Physiology and Disorders
- Biochemical and Structural Characterization
- Erythrocyte Function and Pathophysiology
- Advanced Materials and Mechanics
- Photoreceptor and optogenetics research
Centre National de la Recherche Scientifique
2016-2025
Institut Interdisciplinaire de Neuroscience
2016-2025
Université de Bordeaux
2015-2024
Neurocentre Magendie
2008
Leibniz Institute for Neurobiology
2008
Johns Hopkins Medicine
2008
Johns Hopkins University
2008
Columbia University
2003-2007
The spatiotemporal organization of neurotransmitter receptors in postsynaptic membranes is a fundamental determinant synaptic transmission and information processing by the brain. Using four independent super-resolution light imaging methods EM genetically tagged endogenous receptors, we show that, rat hippocampal neurons, AMPARs are often highly concentrated inside synapses into few clusters ∼70 nm that contain ∼20 receptors. stabilized reversibly these nanodomains diffuse freely outside...
Despite the constant advances in fluorescence imaging techniques, monitoring endogenous proteins still constitutes a major challenge particular when considering dynamics studies or super-resolution imaging. We have recently evolved specific protein-based binders for PSD-95, main postsynaptic scaffold at excitatory synapses. Since synthetic recombinant recognize epitopes not directly involved target protein activity, we consider them here as tools to develop PSD-95 probes. After confirming...
Cells rapidly transduce forces exerted on extracellular matrix contacts into tyrosine kinase activation and recruitment of cytoskeletal proteins to reinforce integrin–cytoskeleton connections initiate adhesion site formation. The relationship between these two processes has not been defined, particularly at the submicrometer level. Using talin1-deficient cells, it appears that talin1 is critical for building early mechanical linkages. Deletion blocked laser tweezers, force-dependent...
We have monitored active movements of the cell circumference on specifically coated substrates for a variety cells including mouse embryonic fibroblasts and T cells, as well wing disk from fruit flies. Despite having different functions being multiple phyla, these types share common spatiotemporal pattern in their normal membrane velocity; we show that protrusion retraction events are organized lateral waves along membrane. These wave patterns indicate both spatial temporal long-range...
Single molecule tracking in live cells is the ultimate tool to study subcellular protein dynamics, but it often limited by probe size and photostability. Because of these issues, long-term proteins confined crowded environments, such as intracellular spaces, remains challenging. We have developed a novel optical consisting 5 nm gold nanoparticles functionalized with small fragment camelid antibodies that recognize widely used green fluorescent (GFPs) very high affinity, which we call...
Abstract Multicolor single-molecule localization microscopy (λSMLM) is a powerful technique to reveal the relative nanoscale organization and potential colocalization between different molecular species. While several standard analysis methods exist for pixel-based images, λSMLM still lacks such standard. Moreover, existing only work on 2D data are usually sensitive organization, very important parameter consider in quantitative SMLM. Here, we present an efficient, parameter-free method 3D...
Neuronal transmission relies on the regulated secretion of neurotransmitters, which are packed in synaptic vesicles (SVs). Hundreds SVs accumulate at boutons. Despite being held together, highly mobile, so that they can be recruited to plasma membrane for their rapid release during neuronal activity. However, how such confinement corroborates with motility remains unclear. To bridge this gap, we employ ultrafast single-molecule tracking (SMT) reconstituted system native and living neurons....
Intermediate filaments (IFs) are involved in key cellular functions including polarization, migration, and protection against large deformations. These related to their remarkable ability extend without breaking, a capacity that should be determined by the molecular organization of subunits within filaments. However, this structure-mechanics relationship remains poorly understood at level. Here, using super-resolution microscopy (SRM), we show vimentin exhibit ~49-nanometer axial repeat both...
We monitored isotropic spreading of mouse embryonic fibroblasts on fibronectin-coated substrates. Cell adhesion area versus time was measured via total internal reflection fluorescence microscopy. Spreading proceeds in well-defined phases. found a power-law growth with distinct exponents a_i three sequential phases, which we denote basal (a_1=0.4+-0.2), continous (a_2=1.6+-0.9) and contractile (a_3=0.3+-0.2) spreading. High resolution differential interference contrast microscopy used to...
A combination of cell culture and animal studies has recently shown that adhesion between neurexins neuroligins played important roles in synapse initiation, maturation, function. Binding neurexin-1β to neuroligin-1 triggers the postsynaptic clustering scaffold density protein 95, but composition timing accumulation glutamate receptors at those nascent contacts remain unclear. Using iontophoresis patch-clamp recordings, we identified functional AMPA (AMPARs) NMDA 95 clusters induced by...