A5041772105- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Immune cells in cancer
- Cellular Mechanics and Interactions
- Lipid Membrane Structure and Behavior
- Single-cell and spatial transcriptomics
- HIV Research and Treatment
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Nanofabrication and Lithography Techniques
- Cytomegalovirus and herpesvirus research
- Atherosclerosis and Cardiovascular Diseases
- Advanced Fluorescence Microscopy Techniques
- RNA Interference and Gene Delivery
- Pancreatic and Hepatic Oncology Research
- interferon and immune responses
- Immunodeficiency and Autoimmune Disorders
- Cell Image Analysis Techniques
- Diabetes and associated disorders
University of Oxford
2016-2025
Nuffield Orthopaedic Centre
2015-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
New York University
2013-2024
Kennedy Center
2015-2024
Institute of Rheumatology
2015-2024
RELX Group (United States)
2023-2024
Science Oxford
2023
University of California, San Francisco
1988-2023
University of Freiburg
2023
The specialized junction between a T lymphocyte and an antigen-presenting cell, the immunological synapse, consists of central cluster cell receptors surrounded by ring adhesion molecules. Immunological synapse formation is now shown to be active dynamic mechanism that allows cells distinguish potential antigenic ligands. Initially, receptor ligands were engaged in outermost nascent synapse. Transport these complexes into was dependent on receptor–ligand interaction kinetics. Finally, stable...
Abstract ICAM-1 is a cell surface glycoprotein originally defined by monoclonal antibody (MAb) that inhibits phorbol ester-stimulated leukocyte aggregation. Staining of frozen sections and immunofluorescence flow cytometry showed intercellular adhesion molecule-1 (ICAM-1) expressed on non-hematopoietic cells such as vascular endothelial cells, thymic epithelial certain other fibroblasts, hematopoietic tissue macrophages, mitogen-stimulated T lymphocyte blasts, germinal center dendritic in...
Homotypic adhesion by phorbol ester-stimulated lymphocytes requires LFA-1 and Mg+2 does not involve like-like interactions between molecules on adjacent cells. The latter finding suggested that a second molecule, distinct from LFA-1, also participates in LFA-1-dependent adhesion. identification of such molecule was the object this investigation. After immunization with LFA-1-deficient EBV-transformed lymphoblastoid cells, MAb obtained inhibits aggregation LFA-1+ EBV lines. This defines novel...
Intercellular adhesion molecule-1 (ICAM-1) on the surface of cultured umbilical vein and saphenous endothelial cells was upregulated between 2.5- 40-fold by rIL-1, rTNF, LPS rIFN gamma corresponding to up 5 X 10(6) sites/cell. Endothelial cell ICAM-1 a single band 90 kD in SDS-PAGE. Purified reconstituted into liposomes bound plastic an excellent substrate for both JY B lymphoblastoid T lymphoblast adhesion. Adhesion planar membranes blocked completely monoclonal antibodies lymphocyte...
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
CD2-associated protein (CD2AP) is an 80-kilodalton that critical for stabilizing contacts between T cells and antigen-presenting cells. In CD2AP-deficient mice, immune function was compromised, but the mice died at 6 to 7 weeks of age from renal failure. kidney, CD2AP expressed primarily in glomerular epithelial Knockout exhibited defects cell foot processes, accompanied by mesangial hyperplasia extracellular matrix deposition. Supporting a role specialized junction known as slit diaphragm,...
The area of contact between a T cell and an antigen-presenting (APC) is known as the immunological synapse. Although its exact function unknown, one model suggests that it allows for receptor (TCR) clustering sustained signaling in cells many hours. Here we demonstrate TCR-mediated tyrosine kinase naı̈ve occurred primarily at periphery synapse was largely abated before mature synapses had formed. These data suggest hours TCR are not required activation. observations challenge current ideas about role
The growth factor progranulin (PGRN) has been implicated in embryonic development, tissue repair, tumorigenesis, and inflammation, but its receptors remain unidentified. We report that PGRN bound directly to tumor necrosis (TNFRs) disturbed the TNFα-TNFR interaction. PGRN-deficient mice were susceptible collagen-induced arthritis, administration of reversed inflammatory arthritis. Atsttrin, an engineered protein composed three fragments, exhibited selective TNFR binding. Atsttrin prevented...
The cell surface expression and function of the LFA-1 ligand, intercellular adhesion molecule 1 (ICAM-1), on epidermal keratinocytes (EK) was studied. ICAM-1 cultured EK either absent or weak, but induced by treating with rIFN-gamma TNF for 4-48 h. IFN-gamma were synergistic. treatment increased T lymphoblast from less than 2% to 20-40%, a concentration dependence similar that seen induction. All inhibited mAbs, not HLA-DR, CD2, LFA-3 mAbs. There no difference in level IFN-gamma-treated...
Abstract Recruitment of effector T cells to inflamed peripheral tissues is regulated by chemokines and their receptors, but the factors regulating recruitment tumors remain largely undefined. Ionizing radiation (IR) therapy a common treatment modality for breast other cancers. Used as cytocidal agent proliferating cancer cells, IR in combination with immunotherapy has been shown promote immune-mediated tumor destruction preclinical studies. In this study we demonstrate that markedly enhanced...
We examined the in vivo behavior of liver natural killer T cells (NKT cells) by intravital fluorescence microscopic imaging mice which a green fluorescent protein cDNA was used to replace gene encoding chemokine receptor CXCR6. NKT cells, account for most CXCR6+ liver, were found crawl within hepatic sinusoids at 10–20 μm/min and stop upon cell antigen activation. CXCR6-deficient exhibited selective severe reduction CD1d-reactive decreased susceptibility T-cell-dependent hepatitis. CXCL16,...