A5082245754- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Virus-based gene therapy research
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Cell Adhesion Molecules Research
- Chemokine receptors and signaling
- Cancer Genomics and Diagnostics
- Advanced Biosensing Techniques and Applications
- Cytokine Signaling Pathways and Interactions
- Systemic Lupus Erythematosus Research
- Cancer, Stress, Anesthesia, and Immune Response
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer, Lipids, and Metabolism
- Cytomegalovirus and herpesvirus research
- Cancer Research and Treatments
- Urticaria and Related Conditions
- Lymphoma Diagnosis and Treatment
- HER2/EGFR in Cancer Research
de Duve Institute
2014-2023
UCLouvain
2013-2022
Walloon Excellence in Lifesciences and Biotechnology
2012-2022
Cliniques Universitaires Saint-Luc
1983-2019
Vrije Universiteit Brussel
2019
KU Leuven
1986-2013
Radboud University Nijmegen
2010-2013
Miltenyi Biotec (Germany)
2013
Radboud University Medical Center
2010-2013
Ludwig Cancer Research
1999-2012
Lymphocytes of melanoma patients can be restimulated in vitro with autologous tumor cells to generate antitumor cytolytic T lymphocytes (CTL). Previous reports have indicated that, when such CTL are obtained from HLA-A2 patients, they often display broad reactivity on A2 cell lines. Such clones, which appeared recognize the same antigen, were isolated two patients. We report here cloning a cDNA that directs expression antigen recognized by these CTL. This corresponds transcript tyrosinase...
It has been reported previously that antitumor cytolytic T lymphocyte (CTL) clones can be isolated from blood lymphocytes of HLA-A2 melanoma patients, after stimulation in vitro with autologous tumor cells, and some these CTL lyse most melanomas. A first antigen recognized by such was shown to encoded the tyrosinase gene. We report here identification another gene also directs expression an on melanomas are restricted HLA-A2. The gene, designated Melan-A, is unrelated any known 18 kb long...
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
Thirty-nine tumor-bearing patients with metastatic melanoma were treated 3 subcutaneous injections of the MAGE-3.A1 peptide at monthly intervals. No significant toxicity was observed. Of 25 who received complete treatment, 7 displayed tumor regressions. All but one these regressions involved cutaneous metastases. Three and 2 led to a disease-free state, which persisted for more than years after beginning treatment. evidence cytolytic T lymphocyte (CTL) response found in blood 4 analyzed,...
A T-cell-derived lymphokine was identified by its ability to support the growth of a subset B-cell hybridomas. Hybrids that failed survive in absence this molecule represented major proportion rat-mouse hybridomas but were very rare among mouse-mouse hybrids. Stable factor-dependent used monitor purification factor from supernatant clonotypically stimulated mouse helper T-cell clone. Sequential fractionation using gel filtration, anion-exchange chromatography, and reversed-phase HPLC...
We have identified an antigen recognized on a human melanoma by autologous cytolytic T lymphocytes. It is encoded gene that expressed in many normal tissues. Remarkably, the sequence coding for antigenic peptide located across exon-intron junction. A point mutation present intron generates amino acid change essential recognition of anti-tumor cytotoxic This observation suggests T-cell-mediated surveillance integrity genome may extend to some intronic regions.
Abstract The human MAGE‐3 gene is expressed in many tumors of several histological types but it silent normal tissues, with the exception testis. Antigens encoded by may, therefore, be useful targets for specific anti‐tumor immunization cancer patients. We reported previously that codes an antigenic peptide recognized on a melanoma cell line autologous cytolytic T lymphocytes (CTL) restricted HLA‐A1. Here we report also another CTL HLA‐A2. peptides bearing consensus anchor residues HLA‐A2...
The immunogenicity of malignant cells has recently been acknowledged as a critical determinant efficacy in cancer therapy. Thus, besides developing direct immunostimulatory regimens, including dendritic cell-based vaccines, checkpoint-blocking therapies, and adoptive T-cell transfer, researchers have started to focus on the overall immunobiology neoplastic cells. It is now clear that can succumb some anticancer therapies by undergoing peculiar form cell death characterized an increased...
Vaccination against cancer by using dendritic cells has for more than a decade been based on generated ex vivo from monocytes or CD34(+) progenitors. Here, we report the first clinical study of therapeutic vaccination naturally occurring plasmacytoid (pDC). Fifteen patients with metastatic melanoma received intranodal injections pDCs activated and loaded tumor antigen-associated peptides vivo. In imaging showed that administered migrated distributed over multiple lymph nodes. Several mounted...
Abstract Lymphoid neogenesis, or the development of lymphoid structures in nonlymphoid organs, is frequently observed chronically inflamed tissues, during course autoimmune, infectious, and chronic graft rejection diseases, which a sustained lymphocyte activation occurs presence persistent antigenic stimuli. The such ectopic has also been reported primary lung, breast, germline cancers, but not yet melanoma. In this study, we structures, defined as follicles comprising clusters B lymphocytes...
Interleukin-HP1 (HP1)/IL-6 is a 25-30-kD protein produced by macrophages, fibroblasts, and certain T cell lines. It was originally identified as mouse growth factor for B hybridomas plasmacytomas, recently shown to stimulate differentiation of normal cells. Here we demonstrate that, in the presence lectins or anti-T receptor antibodies, HP1/IL-6 has activity equivalent that IL-2 mature thymic peripheral cells both L3T4+ Lyt-2+ subsets. Contrary IL-4, was, however, not capable supporting...
Melanoma patients have high frequencies of T cells directed against antigens their tumor. The frequency these antitumor in the blood is usually well above that anti-vaccine observed after vaccination with tumor antigens. In a patient vaccinated MAGE-3 antigen presented by HLA-A1, we measured and several metastases to evaluate respective potential contribution rejection. anti–MAGE-3.A1 was 1.5 × 10−5 CD8 an invaded lymph node, sixfold higher than blood. An cytotoxic lymphocyte (CTL)...
Abstract Human Treg and Th clones secrete the latent form of TGF‐β, in which mature TGF‐β protein is bound to latency‐associated peptide (LAP), thereby prevented from binding receptor. We previously showed that upon TCR stimulation, human but not produce active bear LAP on their surface. Here, we show i.e. both binds glycoprotein A repetitions predominant (GARP), a transmembrane containing leucine rich repeats, present surface stimulated clones. Membrane localization mediated by GARP may be...
Human minor histocompatibility antigens (mHags) play an important role in the induction of cytotoxic T lymphocyte (CTL) reactivity against leukemia after human leukocyte antigen (HLA)-identical allogeneic bone marrow transplantation (BMT). As most mHags are not specific but also expressed by normal tissues, antileukemia is often associated with life-threatening graft-versus-host disease (GVHD). Here, we describe a novel mHag, HB-1, that elicits donor-derived CTL B cell acute lymphoblastic...
After vaccination of melanoma patients with MAGE antigens, we observed that even in the few showing tumor regression, frequency anti-vaccine T cells blood was often either undetectable or <10−5 CD8 cells. This being arguably too low for these to be sole effectors rejection, reexamined contribution recognizing other antigens. The presence such antitumor has been widely reported. To begin assessing their vaccine-induced evaluated five vaccinated patients. cytotoxic lymphocyte (CTL)...
We have studied the patterns of antigens recognized by autologous cytolytic T lymphocytes (CTL) on two melanoma cell lines derived from metastases that were removed patient LB33 at several years distance. Cell line LB33-MEL.A was obtained after surgery in 1988. A large number CTL clones directed against with blood collected 1990. In vitro selection cells resistant to these indicated least five different CTL. Four found be presented HLA-A28, B13, B44 and Cw6, respectively. The remained...
Vaccination of melanoma patients with tumor-specific antigens recognized by cytolytic T lymphocytes (CTL) produces significant tumor regressions in a minority patients. These appear to occur the absence massive CTL responses. To detect low-level responses, we resorted antigenic stimulation blood lymphocyte cultures limiting dilution conditions, followed tetramer analysis, cloning tetramer-positive cells, and T-cell receptor (TCR) sequence analysis clones that showed strict specificity for...