A5089176517- Immunotherapy and Immune Responses
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Congenital Diaphragmatic Hernia Studies
- Preterm Birth and Chorioamnionitis
- High Temperature Alloys and Creep
- nanoparticles nucleation surface interactions
- Microstructure and mechanical properties
- Pregnancy and preeclampsia studies
- Intermetallics and Advanced Alloy Properties
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- DNA Repair Mechanisms
- Birth, Development, and Health
- Monoclonal and Polyclonal Antibodies Research
- Landslides and related hazards
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Lymphoma Diagnosis and Treatment
- Neonatal and fetal brain pathology
- Magnetic properties of thin films
- Advanced Welding Techniques Analysis
Tohoku University Hospital
2017-2025
The University of Western Australia
2022-2024
Spectris (United Kingdom)
2013-2017
National Institute of Technology, Kagoshima College
1990-2014
Tokyo Institute of Technology
2000-2014
The University of Tokyo
2011-2012
Wakayama Medical University
2010-2011
Sapporo Medical University
1993-2009
Dokkyo University
2005-2008
Kushiro City General Hospital
2007
Fanconi anemia (FA) is a rare autosomal recessive cancer susceptibility disorder characterized by cellular hypersensitivity to mitomycin C (MMC). Six FA genes have been cloned, but the gene or corresponding subtypes B and D1 remain unidentified. Here we show that cell lines derived from FA-B FA-D1 patients biallelic mutations in BRCA2 express truncated proteins. Functional complementation of fibroblasts with wild-type complementary DNA restores MMC resistance. Our results link six cloned...
Using molecular dynamics simulations with a many-body force field, we studied the deformation of single crystal Ni and NiCu random alloy nanowires subjected to uniform strain rates but kept at 300 K. For all rates, nanowire is elastic up $7.5%$ yield stress 5.5 GPa, far above that bulk Ni. At high find for both systems crystalline phase transforms continuously an amorphous phase, exhibiting dramatic change in atomic short-range order near vanishing tetragonal shear constant perpendicular...
We have studied the patterns of antigens recognized by autologous cytolytic T lymphocytes (CTL) on two melanoma cell lines derived from metastases that were removed patient LB33 at several years distance. Cell line LB33-MEL.A was obtained after surgery in 1988. A large number CTL clones directed against with blood collected 1990. In vitro selection cells resistant to these indicated least five different CTL. Four found be presented HLA-A28, B13, B44 and Cw6, respectively. The remained...
BACKGROUND: Synovial sarcoma is a high-grade malignant tumor of soft tissue, characterized by the specific chromosomal translocation t(X;18), and its resultant SYT-SSX fusion gene. Despite intensive multimodality therapy, majority metastatic or relapsed diseases still remain incurable, thus suggesting need for new therapeutic options. We previously demonstrated antigenicity gene-derived peptides in vitro analyses. The present study was designed to evaluate vivo immunological property...
Abstract We reported previously a HLA-A24-restricted antigenic peptide, survivin-2B80-88 (AYACNTSTL), recognized by CD8+ CTL. This peptide was derived from survivin protein, an inhibitor of apoptosis proteins, expressed in variety tumors, such as adenocarcinoma, squamous cell carcinoma, and malignant melanoma. In this report, we provide further evidence that might serve potent immunogenic cancer vaccine for various patients. Overexpression detected surgically resected primary tumor specimens...
Antenatal steroid therapy for fetal lung maturation is routinely administered to women at risk of preterm delivery. There strong evidence demonstrate benefit from antenatal steroids in terms survival and respiratory disease, notably infants delivered or below 32 weeks' gestation. However, dosing remains unoptimized benefits are highly variable. Current treatment regimens generate high-concentration, pulsatile exposures now associated with increased childhood neurodevelopmental diseases. We...
Abstract To investigate the immunogenic property of peptides derived from synovial sarcoma-specific SYT-SSX fusion gene, we synthesized four according to binding motif for HLA-A24. The peptides, SS391 (PYGYDQIMPK) and SS393 (GYDQIMPKK), were breakpoint SYT-SSX, SS449a (AWTHRLRER) SS449b (AWTHRLRERK) SSX region. These tested their reactivity with CTL precursors (CTLps) in 16 sarcoma patients using HLA-A24/SYT-SSX peptide tetramers also induction specific CTLs HLA-A24+ patients. Tetramer...
PRAME (Preferentially expressed antigen of melanoma) has been previously identified as a melanoma recognized by cytotoxic T cells (CTLs) and found to be in variety cancer including leukaemic cells. We have screened 98 Japanese patients with leukaemia lymphoma for expression the gene using semiquantitative reverse transcription polymerase chain reaction (RT‐PCR). Forty‐one (42%) showed high levels expression. Eight these were then monitored real‐time PCR period 10–37 months. Significant...
Abstract The prognosis for patients with osteosarcoma who do not respond to current chemotherapy protocols still remains poor. Toward the goal of establishing efficacious peptide-based immunotherapy those patients, we previously developed an autologous pair CTLs and cell line. In study, screened cDNA library this line using CTL clone identified encoding antigen. isolated was identical papillomavirus binding factor (PBF), which recently reported as a DNA transcription cooperating RUNX1....
The DNA double-strand breaks (DSBs) repair pathway has been implicated in maintaining genomic integrity via suppression of chromosomal rearrangements. DNA-dependent protein kinase (DNA-PK) an important role with DSBs repair. In this study, 93 untreated cancer patients and 41 cancer-free healthy volunteers were enrolled. Peripheral blood was collected, separated centrifuged; DNA-PK activity measured by DNA-pull-down assay. expressions DNA-PKcs, Ku70 Ku86 examined RT–PCR assay western...
Abstract Background Antenatal steroid (ANS) therapy is given to women at risk of preterm delivery accelerate fetal lung maturation. However, the benefit ANS variable and how maternal factors contribute this observed variability unknown. We aimed test degree concordance in function, correlate with genomic, transcriptomic, pharmacokinetic variables dizygotic twin ovine fetuses. Methods Thirty-one date-mated ewes carrying fetuses 123 ± 1 days’ gestation received intramuscular injections either...
Abstract In this study, we first categorized nine squamous cell carcinoma (SCC) lines into two groups in terms of the expression HLA-DR, -DP, and -DQ molecules. Subsequently, class II transactivator (CIITA) was studied these lines, because it is widely accepted that MHC molecules regulated by different types CIITA transcripts are initiated distinct promoters. The majority SCC (six nine) expressed HLA-DR promoter IV (pIV) presence IFN-γ. contrast, three were completely negative for all CIITA,...
Abstract Fanconi anemia (FA) is an inherited disease with congenital abnormalities and extreme risk of acute myeloid leukemia (AML). Genetic events occurring during malignant transformation in FA the biology FA‐associated AML are poorly understood, but often preceded by development chromosomal aberrations involving 3q26‐29 bone marrow patients. We report here molecular cytogenetic characterization FA‐derived cell lines SB1685CB SB1690CB conventional array comparative genomic hybridization,...