A5024713540- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer, Hypoxia, and Metabolism
- vaccines and immunoinformatics approaches
- Veterinary Oncology Research
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Glycosylation and Glycoproteins Research
- Angiogenesis and VEGF in Cancer
- Proteoglycans and glycosaminoglycans research
- Rabies epidemiology and control
- Microbial infections and disease research
- Virus-based gene therapy research
- Immune Response and Inflammation
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Signaling Pathways in Disease
- Echinoderm biology and ecology
- Marine Sponges and Natural Products
- Heat shock proteins research
- Seaweed-derived Bioactive Compounds
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
Azabu University
2014-2025
Sapporo Medical University
2000-2012
Tokyo University of Science
2004
University of California, Berkeley
2003
BACKGROUND: Synovial sarcoma is a high-grade malignant tumor of soft tissue, characterized by the specific chromosomal translocation t(X;18), and its resultant SYT-SSX fusion gene. Despite intensive multimodality therapy, majority metastatic or relapsed diseases still remain incurable, thus suggesting need for new therapeutic options. We previously demonstrated antigenicity gene-derived peptides in vitro analyses. The present study was designed to evaluate vivo immunological property...
It is well-established that heat shock proteins (HSPs)-peptides complexes elicit antitumor responses in prophylactic and therapeutic immunization protocols. HSPs such as gp96 Hsp70 have been demonstrated to undergo receptor-mediated uptake by APCs with subsequent representation of the HSP-associated peptides MHC class I molecules on APCs, facilitating efficient cross-presentation. On contrary, despite its abundant expression among cytosol, role Hsp90 for cross-presentation remains unknown....
Extracellular heat shock protein can deliver associated antigens into the MHC class I presentation pathway of antigen-presenting cells, a process called cross-presentation, thus inducing antigen-specific CD8+ T-cell responses; however, precise mechanism for intracellular antigen translocation and processing has not been fully elucidated. Here we demonstrate that cross-presentation extracellular Hsp90–ovalbumin (OVA) complexes to specific T cells involves both classical...
Abstract To investigate the immunogenic property of peptides derived from synovial sarcoma-specific SYT-SSX fusion gene, we synthesized four according to binding motif for HLA-A24. The peptides, SS391 (PYGYDQIMPK) and SS393 (GYDQIMPKK), were breakpoint SYT-SSX, SS449a (AWTHRLRER) SS449b (AWTHRLRERK) SSX region. These tested their reactivity with CTL precursors (CTLps) in 16 sarcoma patients using HLA-A24/SYT-SSX peptide tetramers also induction specific CTLs HLA-A24+ patients. Tetramer...
Background Cyclosporin A (CsA) is well known as an immunosuppressive drug useful for allogeneic transplantation. It has been reported that CsA inhibits hepatitis C virus (HCV) genome replication, which indicates cellular targets of regulate the viral replication. However, regulation mechanisms HCV replication governed by target proteins have not fully understood. Principal Findings Here we show a chemical biology approach elucidates novel mechanism We developed phage display screening to...
Abstract We succeeded in purifying a major glycolipid fraction from green vegetable, spinach. This consists mainly of three glycolipids: monogalactosyl diacylglycerol (MGDG), digalactosyl (DGDG), and sulfoquinovosyl (SQDG). In previous study, we found that the inhibited DNA polymerase activity, cancer cell growth tumor with subcutaneous injection. aimed to clarify oral administration fraction, suppressing colon adenocarcinoma (colon‐26) mice. A graft study showed 20 mg/kg for 2 weeks induced...
ABSTRACT Using a noninvasive DNA methylation (DNAm)–based age estimation method, we investigate the structure of humpback whales that newly emerged around Hachijojima Island, Tokyo Metropolis, Japan to uncover role this area for species. We measured DNAm frequencies at three age‐related genes ( GRIA2 , CDKN2A, and TET2 ) from 26 biopsy skin samples 21 unique (15 males 6 females) randomly collected in winters 2018–2021 estimated their ages using known model whales. The individuals were...
We recently reported that 3′‐sulfonoquinovosyl‐1′‐monoacylglycerol (designatedA‐5) extracted from sea urchin intestine was effective in suppressing the growth of solid tumors. Although major fatty acid component A‐5 a saturated C 16 acid, there were five other acids, 14:0, 18:0, 14:1, 16:1, and 18:1, which constitute minor components A‐5. Therefore, it remains unclear as to these six has anti‐tumor effect. In this study, we synthesized sulfolipids each containing only one acids tested their...
Cancer-associated retinopathy (CAR) is a rare paraneoplastic syndrome, and the recoverin-specific autoantibody suggested to contribute pathogenesis of retinopathy, including apoptosis retinal cells. Because it known that CAR+ cancer patients have preferable prognosis, we hypothesized aberrantly expressed recoverin in cells can become target cytotoxic T lymphocytes (CTL). Here tested nine recoverin-derived HLA-A24-binding peptides for their capacity elicit antitumor CTL. We observed CTL...
Accumulating evidence supporting the cancer stem cell (CSC) hypothesis is based on finding that tumors contain a small population of self-renewing cells generate differentiated progeny and thereby contribute to tumor heterogeneity. CSCs are reported exist in several human cancers, yet only few reports demonstrate existence primary lung dogs. In this study, authors established line derived from canine adenocarcinoma identified side (SP) displayed drug-resistant features. To confirm...
Abstract: We succeeded in purifying the fraction of monogalactosyl diacylglycerol (MGDG), digalactosyl (DGDG), and sulfoquinovosyl (SQDG) containing major glycolipids from a green vegetable, spinach (Spinacia oleracea L.). This inhibited activities replicative DNA polymerases (pols) such as α, δ, ε, mitochondrial pol γ with IC50 values 44.0–46.2 μg/ml, but had no influence on activity repair-related β. The also proliferation human cervix carcinoma (HeLa) cells LD50 57.2 μg/ml. In an vivo...
Background. It is important to develop new immunosuppressive agents without clinical drawbacks. In this article, we reveal the possibility of a chemically synthetic sulfonolipid that acts as novel drug. Methods. We evaluated effect 3-O-(6-deoxy-6-sulfono-β-D-glucopyranosyl)-1,2-di-O-acylglycerol (β-SQDG) contains saturated C18 fatty acid, which designated β-SQDG(18:0) by mixed lymphocyte reaction (MLR) and rat allogeneic skin graft. Then, investigated mechanism β-SQDG(18:0). Results....
Abstract To investigate the effects of anchor substitutions in SYT-SSX junction peptide, an HLA-A24 residue (position 9) B peptide (GYDQIMPKK) was substituted to more favorable residues according HLA-A24-binding motif. Among four substitutes constructed, a substitute with isoleucine (termed K9I peptide) most apparently enhanced affinity for molecule. Subsequent vitro CTL induction analysis using PBMCs 15 HLA-A24+ synovial sarcoma patients revealed that original allowed induce...
Angiogenesis is a promising target for the treatment of cancer, and varying types antiangiogenic agents have been developed. However, limitations problems associated with therapy recently arisen. Although radiotherapy can be combined compounds to overcome these difficulties, almost all previously described angiogenesis inhibitors could still cause side effects at effective doses, only additive are seen in current combination therapy. In this study, we identified member...