A5101821394- Immunotherapy and Immune Responses
- Heat shock proteins research
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Cardiovascular Health and Disease Prevention
- RNA Interference and Gene Delivery
- Cancer Genomics and Diagnostics
- Cancer, Stress, Anesthesia, and Immune Response
- Renal and related cancers
- Cardiovascular and exercise physiology
- Rice Cultivation and Yield Improvement
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Olfactory and Sensory Function Studies
- Immune Response and Inflammation
- Cancer Cells and Metastasis
- vaccines and immunoinformatics approaches
- Toxin Mechanisms and Immunotoxins
- GABA and Rice Research
- DNA Repair Mechanisms
- CAR-T cell therapy research
- Cancer Research and Treatments
- Hepatitis B Virus Studies
- Cancer, Hypoxia, and Metabolism
- Heart Rate Variability and Autonomic Control
Naruto, Tokushima Prefecture hospital
2016-2025
Sapporo Medical University
2011-2024
Western Region Agricultural Research Center
2024
National Agriculture and Food Research Organization
2012-2024
Kyushu Okinawa Agricultural Research Center
2010-2023
Osaka Yukioka Medical University
2022
Hokkaido University
2014-2022
Tokushima University
2015-2020
Japan Sport Council
2020
Japan International Research Center for Agricultural Sciences
2004-2018
Immunotherapy of mice with preexisting cancers heat shock protein preparations derived from autologous cancer resulted in retarded progression the primary cancer, a reduced metastatic load, and prolongation life-span. Treatment other than did not provide significant protection. Spontaneous (lung melanoma), chemically induced (fibrosarcoma colon carcinoma), an ultraviolet radiation-induced spindle cell carcinoma were tested, results support efficacy cancer-derived protein-peptide complexes...
Survivin is a member of the inhibitor apoptosis protein (IAP) family containing single baculovirus IAP repeat domain. It expressed during fetal development but becomes undetectable in terminally differentiated normal adult tissues. We previously reported that survivin and its splicing variant survivin-2B was abundantly various types tumor tissues as well cell lines suitable target antigen for active-specific anti-cancer immunization. Subsequently, we identified an HLA-A24-restricted...
Cancer stem-like cells (CSC) are a small population of cancer with superior tumor initiating, self-renewal, and differentiation properties. In this study, we show that the cancer-testis antigen HSP40 family member DNAJB8 contributes to CSC phenotype in renal cell carcinoma (RCC). overexpression increased percentage side (SP) representing CSCs RCC cells, enhancing their tumor-initiating ability. Conversely, attenuation decreased SP reduced The utility as an immunologic target was established...
Abstract We reported previously a HLA-A24-restricted antigenic peptide, survivin-2B80-88 (AYACNTSTL), recognized by CD8+ CTL. This peptide was derived from survivin protein, an inhibitor of apoptosis proteins, expressed in variety tumors, such as adenocarcinoma, squamous cell carcinoma, and malignant melanoma. In this report, we provide further evidence that might serve potent immunogenic cancer vaccine for various patients. Overexpression detected surgically resected primary tumor specimens...
It is well-established that heat shock proteins (HSPs)-peptides complexes elicit antitumor responses in prophylactic and therapeutic immunization protocols. HSPs such as gp96 Hsp70 have been demonstrated to undergo receptor-mediated uptake by APCs with subsequent representation of the HSP-associated peptides MHC class I molecules on APCs, facilitating efficient cross-presentation. On contrary, despite its abundant expression among cytosol, role Hsp90 for cross-presentation remains unknown....
Cancer-initiating cells (CICs) are thought to be essential for tumor maintenance, recurrence, and distant metastasis, they therefore reasonable targets cancer therapy. Cancer immunotherapy is a novel approach target cancer. In this study, we aimed establish CIC-targeting immunotherapy.Colorectal (CRC) CICs were isolated as side population (SP) cells. The gene expression profile of CRC was analyzed by cDNA microarray RT-PCR. Protein olfactory receptor family 7 subfamily C member 1 (OR7C1)...
One of the major factors involved in prognosis oral squamous cell carcinoma (OSCC) patients is metastasis. Recent progress cancer stem‐like cell/cancer‐initiating (CSC/CIC) research indicates that CSCs are related to Aldehyde dehydrogenase 1 – (ALDH1) and SRY‐related HMG‐box gene 2 (SOX2) have recently been shown be putative CSC markers for several human malignancies. The aim this study was determine association ALDH1 SOX2 expression with lymph node Immunohistochemical staining ALDH1, Ki67...
Abstract Cancer/testis ( CT ) antigens encoded by genes are immunogenic antigens, and the expression of gene is strictly restricted to only testis among mature organs. Therefore, promising candidates for cancer immunotherapy. In a previous study, we identified novel antigen, DNAJB8 . was found be preferentially expressed in stem‐like cells CSCs )/cancer‐initiating CICs ), it thus CSC antigen. this hypothesized that / rather than non‐ /‐ examined The 74 evaluated side population SP (= main MP...
Survivin, a member of the inhibitor apoptosis protein ( IAP ) family containing single baculovirus repeat domain, is highly expressed in cancerous tissues but not normal counterparts. Our group identified an HLA ‐A24‐restricted antigenic peptide, survivin‐2 B 80–88 AYACNTSTL ), that recognized by CD 8 + CTL s and functions as immunogenic molecule patients with cancers various histological origins such colon, breast, lung, oral, urogenital malignancies. Subsequent clinical trials this epitope...
The aim of the present study was to establish cancer stem‐like cell/cancer‐initiating cell ( CSC / CIC )‐targeting immunotherapy. are thought be essential for tumor maintenance, recurrence and distant metastasis. Therefore they reasonable targets therapy. In study, we found that a heat shock protein HSP ) 40 family member, DnaJ (Hsp40) homolog, subfamily B, member 8 DNAJB 8), is preferentially expressed in derived from colorectal CRC cells rather than non‐ . Overexpression enhanced...
We previously investigated a novel heat-inducible transformation-associated cell surface Ag that is expressed on the activated H-ras oncogene-transformed rat fibrosarcoma W31, but not its parental nontransformed fibroblast WFB. This was detected by mAb 067. Herein, we characterized molecular nature of using anti-heat shock protein (HSP) mAb. The accumulated data indicated expression clearly enhanced several stressors, such as TNF, L-azetidine-2-carboxylic acid, and sodium arsenite....
Identification of tumor-associated antigens may facilitate vaccination strategies to treat patients with malignant diseases. We have found that the centrosomal protein, Cep55/c10orf3 acts as a novel breast carcinoma-associated antigen. mRNA was detectable in wide variety tumor cell lines. Expression barely normal tissues except for testis and thymus. Moreover, protein could be detected by monoclonal anti-Cep55/c10orf3 antibody (♯11-55) 69.8% carcinoma, 25% colorectal 57.8% lung carcinoma...
Human endoplasmic reticulum oxidoreductin 1-α (hERO1-α) is an oxidizing enzyme that exists in the and its expression augmented under hypoxia. It regulates a redox state of various kinds protein through reoxidation "client" disulfide isomerase. Interestingly, although hERO1-α normal tissues was comparatively limited, types cancer cells expressed it large amounts. Therefore, we examined role ERO1-α tumor growth using murine breast line 4T1 found knockdown inhibited vivo decreased lung...
Many human cancers have been reported to enhanced expression of the immune checkpoint molecule programmed death-ligand 1 (PD-L1), which binds cell death-1 (PD-1) expressed on cells. PD-L1/PD-1 plays a role in inhibition antitumor immunity by inducing T apoptosis and tolerance. Thus, it is crucial elucidate mechanisms PD-L1 cancer ERO1-α an oxidase located endoplasmic reticulum. It overexpressed variety tumor types disulfide bond formation collaboration with PDI. Here, we investigated...
An experiment was conducted to assess the antioxidant contents and activities of colored rice grains evaluate their nutritive characteristics in terms chemical composition situ ruminal degradation.Ten cultivars (Oryza sativa L.) collected from several areas Japan were studied, control without pigment, maize, barley, wheat used as grains. Their compositions, polyphenol contents, total capacity (TAC), degradation determined.The starch range 73.5% 79.6%, similar that grain. The black red...
CD8(+) CTLs have an essential role in immune response against tumor. Although increasing number of tumor-associated antigens that can be recognized by been identified from human tumors, a limited is known lung cancer. In addition, because some them are expressed noncancerous tissues, there exist limitations their application to tumor immunotherapy. Livin/ML-IAP one recently inhibitor apoptosis protein (IAP) family, which overexpressed melanoma cells. this report, we show aberrantly many...
A novel monoclonal anti‐pan human leukocyte antigen (HLA) class I heavy chain antibody, EMR8‐5, was established. It could detect HLA‐A, ‐B, and ‐C antigens in formalin‐fixed paraffin embedded tissues. By immunohistochemical staining using the EMR8‐5 various cancer tissues from 246 cases were examined for HLA expression. found that expression decreased 20% to 42% of lung cancer, hepatocellular carcinoma, colon renal cell urothelial carcinoma. In contrast, 85% breast had loss or Of 35...
We previously identified a human leukocyte antigen (HLA)-A24-restricted antigenic peptide, survivin-2B80-88, recognized by CD8+ cytotoxic T lymphocytes (CTL). Subsequently, we attempted clinical trials with this epitope peptide alone for some malignancies, resulting in and immunological responses, although their potential was not strong enough routine use as cancer vaccine. In the current study, to assess whether immunogenicity of survivin-2B80-88 could be enhanced other vaccination...