A5069271331- Barrier Structure and Function Studies
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Multiple Sclerosis Research Studies
- Drug Transport and Resistance Mechanisms
- Caveolin-1 and cellular processes
- 3D Printing in Biomedical Research
- Angiogenesis and VEGF in Cancer
- Monoclonal and Polyclonal Antibodies Research
- Atherosclerosis and Cardiovascular Diseases
- Immune Cell Function and Interaction
- Neurological Disease Mechanisms and Treatments
- S100 Proteins and Annexins
- Cerebrospinal fluid and hydrocephalus
- Innovative Microfluidic and Catalytic Techniques Innovation
- Connexins and lens biology
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Systemic Lupus Erythematosus Research
- Cytokine Signaling Pathways and Interactions
- Chemokine receptors and signaling
- Microfluidic and Bio-sensing Technologies
University of Bern
2016-2025
Robert Koch Institute
2018-2025
National Eye Institute
2023
Centre National de la Recherche Scientifique
2023
National Institutes of Health
2023
Université Toulouse III - Paul Sabatier
2023
Inserm
2023
Medical University of Vienna
2023
Cancer Society of New Zealand
2023
University of Auckland
2023
These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed....
The marriage between immunology and cytometry is one of the most stable productive in recent history science. A rapid search PubMed shows that, as July 2017, using "flow immunology" a term yields more than 68 000 articles, first which, interestingly, not about lymphocytes. It might be stated after short engagement, exchange wedding rings officially occurred when idea to link fluorochromes monoclonal antibodies came about. After this, recognizing different types cells became relatively easy...
An active involvement of blood–brain barrier endothelial cell basement membranes in development inflammatory lesions the central nervous system (CNS) has not been considered to date. Here we investigated molecular composition and possible function extracellular matrix encountered by extravasating T lymphocytes during experimental autoimmune encephalomyelitis (EAE). Endothelial contained laminin 8 (α4β1γ1) and/or 10 (α5β1γ1) their expression was influenced proinflammatory cytokines or...
The human blood brain barrier (BBB) is a selective formed by endothelial cells (hBECs), which important to ensure adequate neuronal function and protect the central nervous system (CNS) from disease. development of in vitro BBB models thus utmost importance for drug discovery programs related CNS diseases. Here, we describe method generate model using cord blood-derived hematopoietic stem cells. were initially differentiated into ECs followed induction properties co-culture with pericytes....
Clinical and experimental evidence indicates that inflammatory processes contribute to the pathophysiology of epilepsy, but underlying mechanisms remain mostly unknown. Using immunohistochemistry for CD45 (common leukocyte antigen) CD3 (T-lymphocytes), we show here microglial activation infiltration leukocytes in sclerotic tissue from patients with mesial temporal lobe epilepsy (TLE), as well a model TLE (intrahippocampal kainic acid injection), characterized by spontaneous, nonconvulsive...
Neuronal activity within the central nervous system (CNS) strictly depends on homeostasis and therefore does not tolerate uncontrolled entry of blood components. It has been generally believed that under normal conditions, endothelial blood-brain barrier (BBB) epithelial blood-cerebrospinal fluid (BCSFB) prevent immune cell into CNS. This view recently changed when it was realized activated T cells are able to breach BBB BCSFB perform surveillance Here we propose privilege CNS is established...
Abstract Disruption of the blood–brain and blood–spinal cord barriers (BBB BSCB, respectively) immune cell infiltration are early pathophysiological hallmarks multiple sclerosis (MS), its animal model experimental autoimmune encephalomyelitis (EAE), neuromyelitis optica (NMO). However, their contribution to disease initiation development remains unclear. In this study, we induced EAE in lys-eGFP-ki mice performed single, nonterminal intravital imaging investigate BSCB permeability...