A5042660964- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Mycobacterium research and diagnosis
- Neonatal and fetal brain pathology
- Neonatal Respiratory Health Research
- Single-cell and spatial transcriptomics
- Retinal Development and Disorders
- MicroRNA in disease regulation
- Venous Thromboembolism Diagnosis and Management
- Traumatic Brain Injury and Neurovascular Disturbances
- Burkholderia infections and melioidosis
- Epigenetics and DNA Methylation
- Adenosine and Purinergic Signaling
- Computational Drug Discovery Methods
- Pharmacological Receptor Mechanisms and Effects
- Atrial Fibrillation Management and Outcomes
- Gene Regulatory Network Analysis
- Neuroscience and Neuropharmacology Research
- Cancer-related gene regulation
- X-ray Diffraction in Crystallography
- Infectious Diseases and Mycology
- Immune cells in cancer
- Osteoarthritis Treatment and Mechanisms
- Neonatal and Maternal Infections
- Cancer Research and Treatments
University College Dublin
2016-2025
Conway School of Landscape Design
2025
Universidad de Navarra
2019-2020
Mater Misericordiae University Hospital
2019
Extracellular vesicles (EVs) are nano-sized containing nucleic acid and protein cargo that released from a multitude of cell types have gained significant interest as potential diagnostic biomarkers. Human serum is rich source readily accessible EVs; however, the separation EVs proteins non-EV lipid particles represents considerable challenge. In this study, we compared most commonly used isolation techniques, either alone or in combination, for 200 µl human their present serum. The size...
The marriage between immunology and cytometry is one of the most stable productive in recent history science. A rapid search PubMed shows that, as July 2017, using "flow immunology" a term yields more than 68 000 articles, first which, interestingly, not about lymphocytes. It might be stated after short engagement, exchange wedding rings officially occurred when idea to link fluorochromes monoclonal antibodies came about. After this, recognizing different types cells became relatively easy...
Abstract Osteoarthritis (OA), a chronic disease characterized by articular cartilage degeneration, is leading cause of disability and pain worldwide. In OA, chondrocytes in undergo phenotypic changes senescence, restricting regeneration favouring progression. Similar to other wound-healing disorders, from OA patients show increase the gap junction channel protein connexin43 (Cx43), which regulates signal transduction through exchange elements or recruitment/release signalling factors....
Triple negative breast cancer (TNBC) is an aggressive subtype with relatively poor clinical outcomes and limited treatment options. Chemotherapy, while killing cells, can result in the generation of highly chemoresistant therapeutic induced senescent (TIS) cells that potentially form stem cell niches resulting metastases. Intriguingly, release significantly more extracellular vesicles (EVs) than non-senescent cells. Our aim was to profile EVs harvested from TIS TNBC compared control identify...
MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells culture. In this study we demonstrate that miR-7 targets key regulators the G1 to S phase transition, including Skp2 Psme3, promote increased levels p27KIP temporary growth arrest phase. Simultaneously, down-regulation DNA repair-specific proteins via Rad54L, pro-apoptotic such p53, combined with up-regulation anti-apoptotic factors like p-Akt, promoted cell survival while arrested G1. Thus can...
Abstract Alterations in intracellular Ca 2+ concentration are amongst the most rapid responses to a variety of stimuli mammalian cells. In nervous system particular, occur within nanoseconds. A major challenge analysis is achieve measurements this very fast time frame. To date, dynamic has been monitored by confocal microscopy, plate‐based assays, spectrofluorometry, and flow cytometry, although there issues with number cells analyzed or gaps recording due addition compounds, significant...
Glycosylation is the most common posttranslational modification of proteins and highly reflective changes in environment a cell. Epigenetic modifications to genome are stably transmitted daughter cells without requirement for genetic sequence alterations. Aberrant regulation both epigenetic programming glycosylation patterning integral aspects carcinogenesis. The objective this study was determine interplay between these two complex cellular processes. We demonstrate that global DNA...
We describe a straightforward approach to continuously monitor variety of highly dynamic microbiological processes in millisecond resolution with flow cytometry, using standard bench-top instrumentation. Four main experimental examples are provided, namely: (1) green fluorescent protein expression by antibiotic-stressed Escherichia coli, (2) labeling heat-induced membrane damage an autochthonous freshwater bacterial community, (3) the initial growth response late stationary E. coli cells...
In a recent genome-wide association study, 40 Fleckvieh bulls with exceptionally poor fertility were found to be homozygous for nonsense mutation in the transmembrane protein 95 (TMEM95) encoding gene. Ejaculates from these individuals exhibited normal sperm concentration, morphology, viability, and motility. However, only 1.7% of inseminations resulted pregnancies. The aim this study was examine effect TMEM95 on bovine function vitro. Sperm (mt/mt) males had lower vitro than wild-type...
Bovine tuberculosis (BTB), caused by Mycobacterium bovis, has an annual incidence in cattle of 0.5% the Republic Ireland and 4.7% UK, despite long-standing eradication programmes being place. Failure to achieve complete is multifactorial, but limitations diagnostic tests are significant complicating factors. Previously, we have demonstrated that Fasciola hepatica infection, highly prevalent these areas, induced reduced sensitivity standard for BTB animals co-infected with F. M. bovis. This...
CO2, the primary gaseous product of respiration, is a major physiologic gas, biology which poorly understood. Elevated CO2 feature microenvironment in multiple inflammatory diseases that suppresses immune cell activity. However, little known about CO2-sensing mechanisms and downstream pathways involved. We found elevated correlates with reduced monocyte macrophage migration patients undergoing gastrointestinal surgery reduces vitro. Mechanistically, autocrine gene expression, thereby...
Infiltrating neutrophils are key effector cells in inflammatory bowel disease (IBD) while providing antimicrobial defense and tissue restitution the intestine. The complexity of neutrophil functions local environments underscores our limited understanding how their adaptation tissues influences progression. Here, we demonstrate that recruited murine colitis infection models, idiopathic IBD, chronic granulomatous disease-associated IBD undergo extensive transcriptional reprogramming,...
Long-term memory is formed by alterations in glutamate-dependent excitatory synaptic transmission, which turn regulated synaptosomal protein of 25 kDa (SNAP-25), a key component the soluble N-ethylmaleimide-sensitive factor attachment receptor complex essential for exocytosis neurotransmitter-filled vesicles. Both reduced and excessive SNAP-25 activity has been implicated various disease states that involve cognitive dysfunctions such as attention deficit hyperactivity disorder,...
Recombinant adeno-associated virus (AAV) represents an efficient system for neuronal transduction. However, a potential drawback of AAV is its restricted packaging capacity approximately 5 kb. To bypass this limitation, number dual- and triple-vector strategies divide the transgene(s) between two or three AAVs. The success these approaches relies directly on cotransduction component Although proof concept stratagems has been demonstrated, underlying rate not analyzed quantitatively. In...
Significant advances in gene therapy have enabled exploration of therapies for inherited retinal disorders, many which are preclinical development or clinical evaluation. Gene conditions has led the way this growing field. The loss ganglion cells (RGCs) is a hallmark number disorders. As field matures innovations that aid refining and optimising efficacy demand. under RGC-related when delivered with recombinant adeno associated vectors (AAV), typically been expressed from ubiquitous promoter...
Aim: Circulating immune cell activation is associated with worse outcome in adult and animal models of brain injury. Our aim was to profile the systemic inflammatory response over first week life infants at risk neonatal encephalopathy correlate early neutrophil monocyte endotoxin responses outcome.Methods: Prospective observational study a tertiary referral university hospital including 22 requiring resuscitation birth who had serial (five time points) CD11b (marker adhesion) (intracellular...
We show theoretically and experimentally a mechanism behind the emergence of wide or bimodal protein distributions in biochemical networks with nonlinear input–output characteristics (the dose–response curve) variability abundance. Large cell-to-cell variation can be beneficial to facilitate two distinct groups response levels as opposed graded response. Under circumstances that we quantify mathematically, responses coexist within cellular population, leading distribution. Using flow...
Phosphorylation of specific substrates by protein kinases is a key control mechanism for vital cell-fate decisions and other cellular processes. However, discovering kinase-substrate relationships time-consuming often rather serendipitous. Computational predictions alleviate these challenges, but the current approaches suffer from limitations like restricted kinome coverage inaccuracy. They also typically utilise only local features without reflecting broader interaction context. To address...
This study tested the hypothesis that sperm sialic acid (Sia) is required to reach site of fertilization, and successful fertilization requires recognition Sia from both oocyte occur. In addition, it has recently been reported Siglecs (Sia-binding-immunoglobulin-like lectins) are present on surface. Thus, possibility was sperm-Siglec-mediated also addressed. Sperm exposed neuraminidase (NMase) exhibited lower overall progressive motility, which translated a decreased ability swim through...
Anastomotic leakage (AL) accounts for a major part of in-house mortality in patients undergoing colorectal surgery. Local ischemia and abdominal sepsis are common risk factors contributing to AL characterized by upregulation the hypoxia-inducible factor (HIF) pathway. The HIF pathway is critically regulated HIF-prolyl hydroxylases (PHDs). Here, we investigated significance PHDs effects pharmacologic PHD inhibition (PHI) during anastomotic healing. Ischemic or septic colonic anastomoses were...