A5053898303- Pancreatic function and diabetes
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Diet, Metabolism, and Disease
- Protein Kinase Regulation and GTPase Signaling
- American Environmental and Regional History
- Cell Adhesion Molecules Research
- Archaeology and Natural History
- Lipid metabolism and disorders
- Diabetes Management and Research
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- T-cell and B-cell Immunology
- American History and Culture
- Viral gastroenteritis research and epidemiology
- Immune cells in cancer
- Heat shock proteins research
- Kruppel-like factors research
- Cytokine Signaling Pathways and Interactions
- Pluripotent Stem Cells Research
- Endoplasmic Reticulum Stress and Disease
- Microbial infections and disease research
Uppsala University
2014-2023
Children's Hospital of Philadelphia
2023
University of Northern Colorado
1992-2022
Government of Northern Ireland
2017
Agri Food and Biosciences Institute
2009-2017
Right to Care
2015
Queen's University Belfast
2000-2013
Durham University
2012
Welsh Government
2012
University of Michigan
1984-2011
Angiostatin, a fragment of plasminogen, has been identified and characterized as an endogenous inhibitor neovascularization. We show that angiostatin treatment endothelial cells in the absence growth factors results increased apoptotic index whereas proliferation is unchanged. Angiostatin also inhibits migration tube formation cells. no effect on factor-induced signal transduction but leads to RGD-independent induction kinase activity focal adhesion kinase, suggesting biological effects...
The half-life of insulin mRNA at various glucose concentrations was determined by filter hybridization techniques in isolated rat islets incubated with 'Hlabeled uridine followed a chase incubation 3.3 or 17 m M glucose.High induced greater stabilization than other poly(A)+ RNAs total cellular RNA.In RIN-5F insulinoma cells, an insulin-producing cell line, cholera toxin, but not glucose, mRNA.After 24 h culture actinomycin D aamanitin several concentrations, content decreased comparison to...
Previous studies have shown that the adaptor protein Shb is involved in receptor tyrosine kinase signaling. In this study, we demonstrate phosphorylated an Src-dependent manner upon vascular endothelial growth factor (VEGF) stimulation using porcine aortic cells expressing human VEGF 2 (VEGFR-2) (KDR). co-immunoprecipitation experiments, could detect interaction between and VEGFR-2 telomerase-immortalized microvascular cells. Furthermore, a glutathione S-transferase pull-down assay, Src...
INTRODUCTION 463 STRUCTURE OF THE INSULIN GENE 465 REGULATION EXPRESSION 468 EVOLUTIONARY ASPECTS (PREPRO)lNSULIN 469 SUPERFAMILY ..... .... . .. ... ....... ...... 473
To define the mechanism whereby glucose regulates islet insulin mRNA content, gene transcription rates were determined in islets labeled with [3H]~ridine at low (3.3) or high (17 mM) glucose.Glucose stimulated of total RNA almost 2-fold and 5.6-fold.Addition dibutyryl CAMP to vitro could partially mimic effect on gene-specific transcription.In insulin-producing RIN-5F cell line, did not affect transcription, while cholera toxin acted as a secretagogue increased well.We conclude that exerts...
The biosynthesis of insulin in the islets Langerhans is strongly controlled at translational level by glucose. We have used a variety experimental approaches efforts to dissect mechanisms underlying stimulatory effect To assess its effects on rates peptide-chain elongation, isolated rat were labelled with [3H]leucine different glucose concentrations presence or absence low cycloheximide. Under these conditions, up 5.6 mM, endogenous mRNA did not become rate-limiting for synthesis insulin,...
Bovine tuberculosis (BTB) is a significant and intractable disease of cattle caused by Mycobacterium bovis. In the United Kingdom, despite an aggressive eradication programme, prevalence BTB increasing with unexplained, exponential rise in cases year on year. Here we show study involving 3,026 dairy herds England Wales that there negative association between exposure to common, ubiquitous helminth parasite, Fasciola hepatica diagnosis BTB. The magnitude single intradermal comparative...
Recently it has been postulated that interleukin-1 (IL-1) locally released by infiltrating mononuclear cells may destroy the pancreatic B during development of insulindependent diabetes mellitus. Since IL-1 is a potent inducer interleukin-6 (IL-6) in various cells, conceivable IL-6 second mediator action. In present study effects alone or combination with were studied on islet function vitro after tissue culture and compared observed exposure to only. Rat islets cultured medium RPMI1640 +...
Vascular endothelial (VE)-cadherin in adherens junctions is an essential component of the vascular barrier, critical for tissue homeostasis and implicated diseases such as cancer retinopathies. Inhibitors Src cytoplasmic tyrosine kinase have been applied to suppress VE-cadherin phosphorylation prevent excessive leakage, edema high interstitial pressure. Here we show that Src-related Yes kinase, rather than Src, localized at cell (EC) where it becomes activated a flow-dependent manner....
Fetal pancreatic beta cells demonstrate a deficient insulin release in response to glucose, but the underlying mechanism at cellular level is unknown. By using from 21-day fetal rats we made an attempt clarify mechanism(s) behind this reduced glucose response. In addition measuring release, metabolism, and ATP content, ATP-regulated K+ channels (G channels) voltage-activated Ca2+ currents were investigated with patch-clamp technique. It was thus demonstrated that not sensitive otherwise had...
Stimulation of fibroblast growth factor receptor-1 (FGFR-1) is known to result in phosphorylation tyrosine 766 and the recruitment subsequent activation phospholipase C-γ (PLC-γ). To assess role endothelial cell function, we generated cells expressing a chimeric receptor, composed extracellular domain PDGF receptor-α intracellular FGFR-1. Mutation phenylalanine prevented PLC-γ resulted reduced FRS2 Ras/MEK/MAPK pathway relative wild-type receptor. However, FGFR-1–mediated MAPK was not...
In the present study an attempt was made to further elucidate molecular mechanisms whereby protein kinase C (PKC) modulates p-cell stimulus-secretion coupling.Regulation of Ca2+ channel activity, [Ca2+l,, and insulin release were investigated in both normal pancreatic mouse p-cells similar B-cells deprived PKC activity.[Ca2+li measured with intracellular fluorescent indicator fura-2 activity estimated by whole cell con- figuration patch-clamp technique.To reveal various isoenzymes p-cell,...
L-Glutamine markedly enhancesinsulin release evoked by x.-leucine in rat pancreatic islets.The metabolic situation found the islets exposed to both L- glutamine and L-leucine was investigated.L-Leucine slightly decreased rate of L-glutamine deamidation, inhibited conversion glutamate 2-ketoglutarate transamination, increased oxidative deamination L-glutamate, stimulated recirculation further metabolism 2-ketoglutarate.L-Glutamine 2ketoisocaproate, but more severely 2-ketoisocaproate...
Effects of protein kinase C (PKC) activation on the insulin-secretory process were investigated, by using beta-cell-rich suspensions obtained from pancreatic islets obese-hyperglycaemic mice. The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA), which is known to activate PKC directly, muscarinic-receptor agonist carbamoylcholine and high glucose concentration enhanced phosphorylation a specific 80 kDa substrate in beta-cells. At non-stimulatory concentration, 10 nM-TPA increased...
The overall objective was to compare immunoglobulin G (IgG) concentrations measured by single radial immunodiffusion (sRID) and ELISA-based methods in samples of bovine colostrum transition milk from contrasting breed types (Limousin × Friesian (n = 10) Holstein 10)). Jugular blood were collected at 48 h post-birth beef dairy calves sera harvested subsequent consumption. Absolute IgG values determined ELISA showed poor agreement with mean (SD) using sRID, fixed bias (sRID – ELISA) 31.89...
The major unmet need and crucial challenge hampering the exciting potential of RNAi therapeutics in ophthalmology is to find an effective, safe non-invasive means delivering siRNA cornea. Although all tissues eye are accessible by injection, topical application preferable for frequent treatment regimen that would be necessary siRNA-induced gene silencing. However, ocular surface one more complex biological barriers drug delivery due combined effect short contact time, tear dilution poor...
Global warming and sea level rise are serious threats to agriculture. The negative effects caused by severe salinity include discoloration reduced surface of the leaves, as well wilting due an impaired uptake water from soil roots. Nanotechnology is emerging a valuable ally in agriculture: several studies have indeed already proven role silicon nanoparticles ameliorating conditions plants subjected (a) biotic stressors. Here, we introduce concept phyto-courier: hydrolyzable porous silicon,...