A5052951405- Caveolin-1 and cellular processes
- Angiogenesis and VEGF in Cancer
- Sarcoma Diagnosis and Treatment
- Signaling Pathways in Disease
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Lymphatic System and Diseases
- Head and Neck Surgical Oncology
- RNA modifications and cancer
- Bone health and treatments
- Tumors and Oncological Cases
- Connective tissue disorders research
- Protein Degradation and Inhibitors
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiac Fibrosis and Remodeling
- Cancer-related gene regulation
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Sympathectomy and Hyperhidrosis Treatments
- Protease and Inhibitor Mechanisms
- Cancer, Hypoxia, and Metabolism
- Axon Guidance and Neuronal Signaling
Science for Life Laboratory
2016-2023
Uppsala University
2014-2023
Institut d'Investigació Biomédica de Bellvitge
2010-2021
Centro de Investigación Biomédica en Red de Cáncer
2021
Ajuntament de L’Hospitalet
2016
St. Jude Children's Research Hospital
2010
Georgetown University
2010
CIBBIM-Nanomedicine
2010
Georgetown Lombardi Comprehensive Cancer Center
2010
Abstract Sarcomas are malignant soft tissue and bone tumours affecting adults, adolescents children. They represent a morphologically heterogeneous class of some entities lack defining histopathological features. Therefore, the diagnosis sarcomas is burdened with high inter-observer variability misclassification rate. Here, we demonstrate classification using machine learning classifier algorithm based on array-generated DNA methylation data. This sarcoma trained dataset 1077 profiles from...
The specific role of VEGFA-induced permeability and vascular leakage in physiology pathology has remained unclear. Here we show that depends on signalling initiated via the VEGFR2 phosphosite Y949, regulating dynamic c-Src VE-cadherin phosphorylation. Abolished Y949 mouse mutant Vegfr2(Y949F/Y949F) leads to VEGFA-resistant endothelial adherens junctions a block molecular extravasation. Vessels mice remain sensitive inflammatory cytokines, morphology, blood pressure flow parameters are...
Abstract The vasculature undergoes changes in diameter, permeability and blood flow response to specific stimuli. dynamics interdependence of these responses different vessels are largely unknown. Here we report a non-invasive technique study dynamic events vessel categories by multi-photon microscopy an image analysis tool, RVDM (relative velocity, direction, morphology) allowing the identification their red cell (RBC) parameters. Moreover, Claudin5 promoter-driven green fluorescent protein...
Vascular endothelial (VE)-cadherin in adherens junctions is an essential component of the vascular barrier, critical for tissue homeostasis and implicated diseases such as cancer retinopathies. Inhibitors Src cytoplasmic tyrosine kinase have been applied to suppress VE-cadherin phosphorylation prevent excessive leakage, edema high interstitial pressure. Here we show that Src-related Yes kinase, rather than Src, localized at cell (EC) where it becomes activated a flow-dependent manner....
// Laura Lagares-Tena 1 , Silvia García-Monclús Roser López-Alemany Olga Almacellas-Rabaiget Juan Huertas-Martínez Miguel Sáinz-Jaspeado Mateo-Lozano 2 Carlos Rodríguez-Galindo 3 Santiago Rello-Varona David Herrero-Martín Oscar M. Tirado Sarcoma Research Group, Institut d’Investigació Biomèdica de Bellvitge-IDIBELL, L’Hospitalet Llobregat, Barcelona, Spain Developmental Tumor Biology Laboratory, Hospital Sant Joan...
Abstract Metastasis is the final stage of tumor progression and thought to be responsible for up 90% deaths associated with solid tumors. Caveolin-1 (CAV1) regulates multiple cancer-associated processes related malignant progression. In present study, we tested hypothesis that CAV1 modulates metastatic ability cells from Ewing's sarcoma family tumors (ESFT). First, analyzed expression by immunostaining a tissue microarray containing 43 paraffin-embedded ESFT known EWS translocations. Even...
Angiogenesis is the result of combined activity tumor microenvironment and signaling molecules. The angiogenic switch represented as an imbalance between pro- anti-angiogenic factors a rate-limiting step in development tumors. Eph receptor tyrosine kinases their membrane-anchored ligands, known ephrins, constitute largest kinase (RTK) subfamily are considered major family pro-angiogenic RTKs. Ewing sarcoma (EWS) highly aggressive bone soft tissue affecting children young adults. As other...
Report23 September 2019Open Access Source DataTransparent process Myc-dependent endothelial proliferation is controlled by phosphotyrosine 1212 in VEGF receptor-2 Chiara Testini Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Science for Life Uppsala University, Uppsala, Sweden Search more papers this author Ross O Smith Yi Jin Pernilla Martinsson Ying Sun Marie Hedlund Miguel Sáinz-Jaspeado Masabumi Shibuya Institute Physiology Medicine, Jobu Takasaki, Gunma, Japan...
PALMD (palmdelphin) belongs to the family of paralemmin proteins implicated in cytoskeletal regulation. Single nucleotide polymorphisms locus that result reduced expression are strong risk factors for development calcific aortic valve stenosis and predict severity disease.Immunodetection public database screening showed dominant endothelial cells (ECs) brain cardiovascular tissues including valves. Mass spectrometry, coimmunoprecipitation, immunofluorescent staining allowed identification...
Sarcomas are a heterogeneous group of mesenchymal malignancies that very often lead to death. Nowadays, chemotherapy is the only available treatment for most sarcomas but there few active drugs and clinical results still remain poor. Thus, an imperious need find new therapeutic alternatives in order improve sarcoma patient’s outcome. During last years, have been described number molecular pathways allowed us know more about cancer biology tumorigenesis. one tumors which advances made....
// Juan Huertas-Martínez 1 , Santiago Rello-Varona David Herrero-Martín Ignasi Barrau Silvia García-Monclús Miguel Sáinz-Jaspeado Laura Lagares-Tena Yaiza Núñez-Álvarez 5 Mateo-Lozano 2 Jaume Mora Josep Roma 3 Nuria Toran Sebastian Moran 4 Roser López-Alemany Soledad Gallego Manel Esteller A. Peinado Xavier García del Muro and Oscar M. Tirado Sarcoma research group, Molecular Oncology Lab, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain...
We conducted a phase I study in patients with advanced solid tumours to identify the recommended dose, assess pharmacokinetics (PK), pharmacodynamic activity and preclinical antitumour efficacy of combination sirolimus gemcitabine. Nineteen were treated 2 or 5 mg daily gemcitabine 800 1000 m−2 on days 1 8. Dose escalation depended dose-limiting toxicity (DLT) rate during first 3-week period. Paired skin biopsies evaluated for phosphorylated S6 (pS6) as marker mTOR (mammalian target...
The acute phase of myocardial infarction (MI) is accompanied by edema contributing to tissue damage and disease outcome. Here, we aimed identify the mechanism whereby vascular endothelial growth factor (VEGF)-A induces in MI eventually promote development therapeutics specifically suppress VEGFA-regulated permeability while preserving collateral vessel formation.VEGFA regulates activation VEGF receptor-2 (VEGFR2), leading induction several signaling pathways including cytoplasmic tyrosine...
The endothelial junction component vascular (VE)-cadherin governs junctional dynamics in the blood and lymphatic vasculature. Here, we explored how stability is modulated by elevated VEGFA signaling to facilitate metastasis sentinel lymph nodes. Zippering of VE-cadherin junctions was established dermal initial vessels after injection tumor-proximal lymphatics mice. Shape analysis pan-cellular fragments revealed that zippering accompanied accumulation small round-shaped endothelium. In mice...
Abstract Rhabdomyosarcoma is the most common soft tissue sarcoma of childhood and adolescence. Despite advances in therapy, patients with a histological variant rhabdomyosarcoma known as alveolar (ARMS) have 5-year survival <30%. Caveolin-1 (CAV-1), encoding structural component cellular caveolae, suggested tumor suppressor gene involved cell signaling. We report that CAV1 expressed embryonic (ERMS) lines samples. However, 3 out 4 different ARMS 8 9 clinical samples expression either...
Introduction: Aberrant DNA methylation is thought to be closely related the development of cancer. Therefore, identification specific markers in Ewing sarcoma (ES) and Rhabdomyosarcoma (RMS) would helpful for understanding pathogenetic mechanism as well developing new therapies. We analyzed methylome ES RMS tumors cell lines.
Abstract Pediatric sarcomas represent a diverse group of rare bone and soft tissue malignancies. Although the molecular mechanisms that propel development these cancers are not well understood, identification tumor-specific translocations in many has provided significant insight into their tumorigenesis. Besides acting as direct modulator transcription, fusion proteins appear to exert its oncogenic functions by epigenetic modifications on transcriptome. Aberrant DNA methylations thought be...
Abstract Ewing sarcoma (ES) is the second most common bone tumor in childhood. ES harbors a characteristic gene translocation that gives rise to fusion protein, commonly EWS/FLI1 (EF). Caveolin-1 (CAV1) direct target of EF, it overexpressed and has an oncogenic role. CAV1 Polymerase I transcript release factor (PTRF) interact at plasma membrane are essential for caveolae formation. The methylome analysis samples cell lines revealed hypermethylation N-shore islands PTRF promoter compared...