A5047418884- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- Immune responses and vaccinations
- Infectious Diseases and Tuberculosis
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- Wildlife Ecology and Conservation
- Diagnosis and treatment of tuberculosis
- Cancer-related molecular mechanisms research
- Listeria monocytogenes in Food Safety
- RNA modifications and cancer
- Zoonotic diseases and public health
- RNA and protein synthesis mechanisms
- COVID-19 epidemiological studies
- interferon and immune responses
- Immune Cell Function and Interaction
- Data-Driven Disease Surveillance
- Escherichia coli research studies
- COVID-19 Digital Contact Tracing
- Antibiotic Resistance in Bacteria
- Veterinary medicine and infectious diseases
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
University College Dublin
2016-2025
Massey University
1995-2006
University of Alberta
1996
Institut Pasteur
1989-1991
Trinity College Dublin
1988
The plasmid RSF1010 belongs to a class of plasmids (IncQ) that replicate in range bacterial hosts. Although non-self-transmissible, it can be mobilized at high frequency between different gram-negative species if transfer functions are supplied trans. We report the by conjugation from Escherichia coli gram-positive actinomycetes Streptomyces lividans and Mycobacterium smegmatis. In its new hosts, was stable with respect structure inheritance conferred high-level resistance streptomycin...
As a consequence of continued spillover Mycobacterium bovis into cattle from wildlife reservoirs and increased globalization trade with associated transmission risks, new approaches such as vaccination novel testing algorithms are seriously being considered by regulatory agencies for the control bovine tuberculosis. Serologic tests offer opportunities identification M. bovis-infected animals not afforded current diagnostic techniques. The present study describes assay development field...
Bovine tuberculosis is a significant veterinary and financial problem in many parts of the world. Although factors influence infection progression disease, there host genetic component dissection this may enlighten on wider biology response to tuberculosis. However, binary phenotype presence/absence presents noisy signal for genomewide association study.We calculated composite merit TB susceptibility based disease incidence daughters elite sires used artificial insemination Irish dairy herd....
A field trial was conducted to investigate the impact of oral vaccination free-living badgers against natural-transmitted Mycobacterium bovis infection. For a period three years were captured over seven sweeps in zones and assigned for with lipid-encapsulated BCG vaccine (Liporale-BCG) or placebo. Badgers enrolled Zone administered placebo while all C vaccinated BCG. middle area, B, randomly 50:50 treatment Treatment each zone remained blinded until end study period. The outcome interest...
The popularity of tri-axial accelerometer data loggers to quantify animal activity through the analysis signature traces is increasing. However, there no consensus on how process large sets that these devices generate when recording at necessary high sample rates. In addition, have been few attempts validate with specific behaviours in non-domesticated terrestrial mammals. We fitted a collar tame captive Eurasian badger (Meles meles). was allowed move freely an outside enclosure and...
Members of the Mycobacterium tuberculosis complex (MTBC) are causative agents in a range mammals, including humans. A key feature MTBC pathogens is their high degree genetic identity yet distinct host tropism. Notably, while bovis highly virulent and pathogenic for cattle, human pathogen M. attenuated cattle. Previous research also suggests that preference amongst members has basis innate immune responses. To explore tropism, we present in-depth profiling reference strains AF2122/97 H37Rv at...
ABSTRACT PCR primers specific for the Mycobacterium tuberculosis complex were used to detect presence of bovis BCG (Pasteur) in soil microcosms and environmental samples taken from a farm Ireland with history bovine tuberculosis. M. genes detected at 4 21 months after possible contamination. Gene levels found range 1 × 10 3 3.6 gene copies g −1 , depending on sampling area. Areas around badger setts had highest detectable shown have persistence. -specific 16S rRNA sequences detected,...
Mycobacterium avium subspecies paratuberculosis is the causative agent of Johne's disease, an intestinal disease ruminants with major economic consequences. Infectious bacilli are phagocytosed by host macrophages upon exposure where they persist, resulting in lengthy subclinical phases infection that can lead to immunopathology and dissemination. Consequently, analysis macrophage transcriptome response M. subsp. provide valuable insights into molecular mechanisms underlie disease. Here, we...
Bovine tuberculosis (BTB), caused by infection with Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many countries. The development high-throughput functional genomics technologies, including gene expression microarrays RNA sequencing (RNA-seq), has enabled detailed analysis host transcriptome to M. bovis infection, particularly at macrophage peripheral blood level. In present...
ABSTRACT It is estimated that more than 50 million cattle are infected with Mycobacterium bovis worldwide, resulting in severe economic losses. Current diagnosis of tuberculosis (TB) relies on tuberculin skin testing, and when combined the slaughter test-positive animals, it has significantly reduced incidence bovine TB. The failure to eradicate TB Great Britain been attributed part a reservoir infection badgers ( Meles meles ). Accurate reliable cornerstone control. Bacteriological these...
Abstract Background Bovine tuberculosis is an enduring disease of cattle that has significant repercussions for human health. The advent high-throughput functional genomics technologies facilitated large-scale analyses the immune response to this may ultimately lead novel diagnostics and therapeutic targets. Analysis mRNA abundance in peripheral blood mononuclear cells (PBMC) from six Mycobacterium bovis infected non-infected controls was performed. A targeted immunospecific bovine cDNA...