A5012230366- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Malaria Research and Control
- Immunodeficiency and Autoimmune Disorders
- Systemic Lupus Erythematosus Research
- HIV Research and Treatment
- Immunotherapy and Immune Responses
- Hepatitis C virus research
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- Enzyme Structure and Function
- Complement system in diseases
- Trypanosoma species research and implications
- Diabetes and associated disorders
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Protein purification and stability
- Hepatitis B Virus Studies
- Lymphoma Diagnosis and Treatment
- Renal Diseases and Glomerulopathies
- Single-cell and spatial transcriptomics
- Atherosclerosis and Cardiovascular Diseases
German Cancer Research Center
2016-2025
Heidelberg University
2015-2024
Cancer Research Center
2023
New York Academy of Sciences
2023
John Wiley & Sons (Germany)
2023
Moss Landing Marine Laboratories
2023
Hudson Institute
2023
Rockefeller University
2003-2020
Karolinska Institutet
2018
Karolinska University Hospital
2018
During B lymphocyte development, antibodies are assembled by random gene segment reassortment to produce a vast number of specificities. A potential disadvantage this process is that some the produced self-reactive. We determined prevalence self-reactive antibody formation and its regulation in human cells. majority (55 75%) all expressed early immature cells displayed self-reactivity, including polyreactive anti-nuclear Most these autoantibodies were removed from population at two discrete...
A cardinal feature of systemic lupus erythematosus (SLE) is the development autoantibodies. The first autoantibodies described in patients with SLE were those specific for nuclei and DNA, but subsequent work has shown that individuals this disease produce a panoply different Thus, one constant features profound breakdown tolerance antibody system. appearance self-reactive antibodies precedes clinical disease, where B cell pathway broken not been defined. In healthy humans, are removed from...
See Zekeridou and Lennon (doi: 10.1093/aww213 ) for a scientific commentary on this article . Anti- N -methyl-D-aspartate receptor (NMDAR) encephalitis is recently discovered autoimmune syndrome associated with psychosis, dyskinesias, seizures. Little known about the cerebrospinal fluid autoantibody repertoire. Antibodies against NR1 subunit of NMDAR are thought to be pathogenic; however, direct proof lacking as previous experiments could not distinguish contribution further anti-neuronal...
Mucosal antibody responses play a major role in mediating homeostasis with the intestinal flora. It has been suggested that imbalance IgA+ and IgG+ B cell repertoire may be associated development of diseases such as inflammatory bowel disease. Despite this, little is known about specificity human plasmablasts. Here, we have determined reactivity profile single isolated plasmablasts from terminal ileum using cloning vitro expression. We found approximately 25% IgA IgG plasmablast antibodies...
B cell chronic lymphocytic leukemia (CLL) is a disease of expanding monoclonal cells whose receptor (BCR) mutational status defines 2 subgroups; patients with mutated BCRs have more favorable prognosis than those unmutated BCRs. CLL express restricted BCR repertoire including antibodies quasi-identical complementarity-determining region 3 (CDR3), which suggests specific antigen recognition. The antigens recognized by may include autoantigens since about half produce autoreactive antibodies....
Splenectomized individuals are prone to overwhelming infections with encapsulated bacteria and splenectomy of mice increases susceptibility streptococcal infections, yet the exact mechanism by which spleen protects against such is unknown. Using congenitally asplenic as a model, we show that essential for generation B-1a cells, B cell population cooperates innate immune system control early bacterial viral growth. Splenectomy wild-type further demonstrated also important survival cells....
Persistent autoantibody production in patients with systemic lupus erythematosus (SLE) suggests the existence of autoreactive humoral memory, but frequency self-reactive memory B cells SLE has not been determined. Here, we report on reactivity 200 monoclonal antibodies from single IgG+ four patients. The overall polyreactive and HEp-2 this compartment was similar to controls. We found 15% IgG cell highly reactive specific for SLE-associated extractable nuclear antigens (ENA) Ro52 La one...
Autoantibodies are removed from the repertoire at two checkpoints during B cell development in bone marrow and periphery. Despite these checkpoints, up to 20% of antibodies expressed by mature naive cells healthy humans show low levels self-reactivity. To determine whether self-reactive also part antigen-experienced memory compartment, we analyzed recombinant cloned single circulating human IgM+ cells. Cells expressing specific for individual bacterial polysaccharides were expanded...
Antibodies can protect from Plasmodium falciparum (Pf) infection and clinical malaria disease. However, in the absence of constant reexposure, serum immunoglobulin (Ig) levels rapidly decline full protection symptoms is lost, suggesting that B cell memory functionally impaired. We show at single level natural Pf induces development classical cells (CM) atypical (AtM) produce broadly neutralizing antibodies against blood stage parasites. CM AtM contribute to anti-Pf IgG production, but only...
Antibodies targeting citrullinated proteins (ACPAs [anticitrullinated protein antibodies]) are commonly found in patients with rheumatoid arthritis (RA), strongly associate distinct HLA-DR alleles, and predict a more aggressive disease course as compared seronegative patients. Still, many features of these antibodies, including their site production the extent MHC class II-driven T cell help, remain unclarified. To address questions, we have used single B cell-based cloning technology to...
The clonal selection of potent germline antibodies outpaces affinity maturation in human anti-PfCSP memory B cell responses.
Research Article11 October 2016Open Access Source DataTransparent process Human isotype-dependent inhibitory antibody responses against Mycobacterium tuberculosis Natalie Zimmermann Group Molecular Immunology, Max Planck Institute for Infection Biology, Berlin, Germany Department of B Cell German Cancer Center, Heidelberg, Search more papers by this author Verena Thormann Bo Hu Anne-Britta Köhler Aki Imai-Matsushima Camille Locht U1019 - UMR 8204 CIIL Centre and Immunity Lille, University...