A5030145457- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- Vector-borne infectious diseases
- vaccines and immunoinformatics approaches
- Parasites and Host Interactions
- HIV Research and Treatment
- Viral Infections and Vectors
- Invertebrate Immune Response Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Trypanosoma species research and implications
- Computational Drug Discovery Methods
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Insect symbiosis and bacterial influences
- Reproductive tract infections research
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Toxin Mechanisms and Immunotoxins
- Parasite Biology and Host Interactions
- Drug Transport and Resistance Mechanisms
- Antibiotic Resistance in Bacteria
- Dermatological diseases and infestations
- Tuberculosis Research and Epidemiology
- Research on Leishmaniasis Studies
Statens Serum Institut
2016-2025
Copenhagen University Hospital
2015-2025
University of Copenhagen
2016-2025
Rigshospitalet
2016-2025
Dansk Sygehus Institut
2017
Institut de génétique et de développement de Rennes
2013
Instituto Evandro Chagas
2005
Institut Pasteur
2005
Centro Nacional de Primatas
2005
Virginia Commonwealth University Medical Center
2003-2004
It is now emerging that for vaccines against a range of diseases including influenza, malaria and HIV, the induction humoral response insufficient substantial complementary cell-mediated immune necessary adequate protection. Furthermore, some such as tuberculosis, cellular seems to be sole effector mechanism required The development new adjuvants capable inducing highly complex responses with strong antigen-specific T-cell in addition antibodies therefore urgently needed.Herein, we describe...
Abstract The rapid development of a SARS-CoV-2 vaccine is global priority. Here, we develop two capsid-like particle (CLP)-based vaccines displaying the receptor-binding domain (RBD) spike protein. RBD antigens are displayed on AP205 CLPs through split-protein Tag/Catcher, ensuring unidirectional and high-density display RBD. Both soluble recombinant bind ACE2 receptor with nanomolar affinity. Mice vaccinated or CLP-displayed RBD, formulated in Squalene-Water-Emulsion. RBD-CLP induce higher...
Background Surrogate markers of protective immunity to malaria in humans are needed rationalize vaccine discovery and development. In an effort identify such markers, thereby provide a clue the complex equation development is facing, we investigated relationship between protection acquired through exposure field with naturally occurring immune responses (i.e., induced by parasite) molecules that considered as valuable candidates. Methods Findings We analyzed, under comparative conditions,...
Antibodies can protect from Plasmodium falciparum (Pf) infection and clinical malaria disease. However, in the absence of constant reexposure, serum immunoglobulin (Ig) levels rapidly decline full protection symptoms is lost, suggesting that B cell memory functionally impaired. We show at single level natural Pf induces development classical cells (CM) atypical (AtM) produce broadly neutralizing antibodies against blood stage parasites. CM AtM contribute to anti-Pf IgG production, but only...
There is longstanding evidence that immunoglobulin G (IgG) has a role in protection against clinical malaria, and human antibodies of the cytophilic subclasses are thought to be particularly critical this respect. In cohort study, 286 Burkinabè children 6 months 15 years old were kept under malaria surveillance order assess protective antibody responses four antigens which currently being evaluated as vaccine candidates: apical membrane antigen 1 (AMA1), merozoite surface protein 1-19...
Antigen-specific antibody-mediated immune responses play an important role in natural protection against clinical malaria, but conflicting estimates of this association have emerged from immuno-epidemiological studies different geographical settings. This study was aimed at assessing a standardized manner the relationship between antibody to four malaria vaccine candidate antigens and cohort Ghanaian children. Standardized ELISA protocols were used measure isotype IgG subclass levels Apical...
Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested an infectious blood meal. Here, we determine transmission-reducing activity (TRA) of naturally acquired from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated antibody responses to Pfs48/45, Pfs230, and 43 novel gametocyte proteins assessed by protein microarray. In field-based assays likelihood rate...
BACKGROUNDMalaria transmission-blocking vaccines aim to interrupt the transmission of malaria from one person another.METHODSThe candidates R0.6C and ProC6C share 6C domain Plasmodium falciparum sexual-stage antigen Pfs48/45. utilizes glutamate-rich protein (GLURP) as a carrier, includes second (Pfs230-Pro) short 36-amino acid circumsporozoite (CSP) sequence. Healthy adults (n = 125) malaria-endemic area Burkina Faso were immunized with 3 intramuscular injections, 4 weeks apart, 30 μg or 100...
ABSTRACT The early secreted antigenic target 6-kDa protein (ESAT-6) and culture filtrate 10 (CFP-10) are promising antigens for reliable immunodiagnosis of tuberculosis. Both encoded by RD1, a genomic region present in all strains Mycobacterium tuberculosis M. bovis but lacking bacillus Calmette-Guérin vaccine strains. Production purification recombinant laborious costly, precluding rapid large-scale testing. Aiming to develop alternative diagnostic reagents, we have investigated whether...
The human T-cell recognition of the low-molecular-mass culture filtrate antigen TB10.4 was evaluated in detail. molecule strongly recognized by T cells isolated from tuberculosis (TB) patients and BCG-vaccinated donors. epitopes on were mapped with overlapping peptides found to be distributed throughout molecule. broadest response TB patients, whereas donors focused mainly toward a dominant epitope located N terminus (amino acids 1 18). gene encoding belong subfamily within esat-6 family...
ABSTRACT We performed a longitudinal clinical and parasitological follow-up study in OoDo, village southeast Asia which malaria is hyperendemic, order to assess the association between protection against attacks antibodies three currently evaluated vaccine candidates, merozoite surface protein 1 (MSP1), MSP3, 220-kDa glutamate-rich (GLURP) from Plasmodium falciparum. Our results showed that levels of cytophilic immunoglobulin G3 (IgG3) conserved regions MSP3 GLURP were significantly...
ABSTRACT The goal of this study was to analyze antibody responses Plasmodium falciparum glutamate-rich protein (GLURP) using clinical data and plasma samples obtained from villagers Dielmo, Senegal. This molecule chosen because it is targeted by human antibodies which induce parasite growth inhibition in antibody-dependent cellular (ADCI) assays. results showed a strong correlation between protection against malaria attacks levels immunoglobulin G2 (IgG2) IgG3 GLURP 94–489 (R0) 705–1178 (R2)...
Antibodies to selected Plasmodium falciparum merozoite antigens are often reported be associated with protection from malaria in one epidemiological cohort, but not another. Here, we sought understand this paradox by exploring the hypothesis that a threshold concentration of antibodies is necessary for protection. We analyzed data two independent cohorts along Kenyan coast, which AMA1, MSP-2 and MSP-3 were (Chonyi) another association was observed (Junju). used reference reagent standardize...
Abstract Background GMZ2 is a recombinant malaria vaccine inducing immune responses against Plasmodium falciparum (Pf) merozoite surface protein-3 and glutamate-rich protein. We used standardized controlled human infection (CHMI) to assess the efficacy of this asexual blood-stage vaccine. Methods vaccinated 50 healthy, adult volunteers with lifelong exposure Pf 3 times, at 4-week intervals, 30 or 100 µg formulated in CAF01, liposome-based adjuvant; GMZ2, Alhydrogel; control (Verorab)....
The Plasmodium falciparum Pfs230 and Pfs48/45 proteins are expressed during transmission from man to mosquito leading candidates for a malaria blocking vaccine. Individually they generate (TB) antibodies in rodent models. Whether the single protein vaccines suitable use field settings will primarily depend on their potency elicit functional antibodies. We hypothesized that combination of both be more potent than each individually. Therefore we designed chimeric composed fragments as well...
ABSTRACT Monocyte-dependent as well direct inhibitory effects of antimalarial antibodies point toward antigens accessible at the time merozoite release targets for biologically active capable mediating protection against Plasmodium falciparum . The glutamate-rich protein (GLURP), being an antigen associated with mature schizont-infected erythrocytes, was therefore object present investigation, in which we analyzed whether anti-GLURP can either interfere directly invasion or act indirectly by...
A recombinant Plasmodium falciparum glutamate-rich protein (GLURP) was produced in Escherichia coli as a nearly full-length protein. In order to map immunodominant regions on GLURP, the nonrepetitive amino-terminal region (R0) well central repeat (R1) and carboxy-terminal (R2) were also separate products. All four purified gene products reacted specifically with serum samples from adults living an area of Liberia where malaria is holoendemic. It appears that human immune response against...
The nucleotide sequences of the ospC gene from five Danish human Borrelia burgdorferi isolates representing all three B. genospecies (B. sensu stricto, garinii sp. nov., and group VS461) American type strain B31 were determined compared with published sequence German isolate PKo (R. Fuchs, S. Jauris, F. Lottspeich, V. Preac-Mursic, Wilske, E. Soutschek, Mol. Microbiol. 6:503-509, 1992). was present in isolates, regardless presence or absence its product, OspC. deduced amino acid OspC seven...
ABSTRACT Immune evasion by Lyme spirochetes is a multifactorial process involving numerous mechanisms. The OspE protein family undergoes antigenic variation during infection and binds factor H (fH) possibly FHL-1/reconectin. In Borrelia burgdorferi B31MI, the consists of three paralogs: BBL39 (ErpA), BBP38, BBN38 (ErpP). BBP38 are identical therefore referred to here as BBL39. goals this study were assess specificity antibody (Ab) response paralogs identify domains or determinants that...
The development of effective malaria vaccines depends on the identification targets well-defined protective immune responses. Data and samples from a longitudinal study cohort children coastal Ghana were used to investigate role antibody responses 3 regions Plasmodium falciparum glutamate-rich protein (GLURP). data show that levels GLURP-specific IgG occurs in nonrepeat region antigen are significantly correlated with clinical protection P. malaria, after correction for confounding effect...