A5001750059- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Immune Response and Inflammation
- Complement system in diseases
- Hematopoietic Stem Cell Transplantation
- interferon and immune responses
- Multiple Myeloma Research and Treatments
- Chemokine receptors and signaling
- Immune cells in cancer
- Cell Adhesion Molecules Research
- vaccines and immunoinformatics approaches
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Dietary Effects on Health
- Lipid Membrane Structure and Behavior
- Acute Myeloid Leukemia Research
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Lymphatic System and Diseases
- Medicinal Plant Pharmacodynamics Research
Albert Einstein College of Medicine
2016-2025
New York University
2009-2013
NYU Langone Health
2013
Yeshiva University
2013
Rockefeller University
2010
Johns Hopkins University
2003-2009
Colorado State University
2007
Columbia University
2003
Carnegie Mellon University
1998
Responses to cholesterol depletion are often taken as evidence of a role for lipid rafts in cell function. Here, we show that has global effects on and plasma membrane architecture The lateral mobility proteins is reduced when chronically or acutely depleted. change consequence the reorganization actin. Binding GFP-tagged pleckstrin homology domain specific phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] after depletion. This result implies cytoskeleton depends loss redistribution...
The germinal center (GC) reaction is essential for the generation of somatically hypermutated, high-affinity antibodies that mediate adaptive immunity. Entry into GC limited to a small number B cell clones; however, process by which this clones selected unclear. In study, we demonstrate low-affinity cells intrinsically capable seeding fail expand and become activated in presence higher-affinity even before coalescence. Live multiphoton imaging shows selection based on amount peptide–major...
Abstract Acute myeloid leukemia (AML) is initiated and sustained by a hierarchy of stem cells (LSCs), elimination this cell population required for curative therapies. Here we show that transmembrane immunoglobulin domain containing 2 (TMIGD2), recently discovered co-stimulatory immune receptor, aberrantly expressed human AML cells, can be used to identify enrich functional LSCs. We demonstrate TMIGD2 the development maintenance self-renewal LSCs but not essential normal hematopoiesis....
The role of lymphatic vessels is to transport fluid, soluble molecules, and immune cells the draining lymph nodes. Here, we analyze how aging process affects functionality collectors dynamics flow. Ultrastructural, biochemical, proteomic analysis indicates a loss matrix proteins, smooth muscle in aged resulting decrease contraction frequency, systolic flow velocity, pumping activity, as measured vivo collectors. Functionally, this impairment also translated into reduced ability for bacterial...
Malaria remains a global health burden causing significant morbidity, yet the mechanisms underlying disease outcomes and protection are poorly understood. Herein, we analyzed peripheral blood of unique cohort Malawian children with severe malaria, performed comprehensive overview leukocytes inflammatory mediators present in these patients. We reveal robust immune cell activation, notably CD14+ monocytes, NK cells plasmacytoid dendritic (pDCs) that is associated very high inflammation. Using...
Abstract The canonical plasma cell marker CD138 (syndecan-1) is highly expressed on the myeloma surface, but its functional role in vivo unclear, as well ontogeny of CD138-high and CD138-negative (neg) cells. In this study we used an murine Vk*MYC model where heterogeneously depending tumor size. We find that comparison to CD138-neg cells, subset cells proliferative, less apoptotic, enhanced IL-6R signaling, which known promote survival. addition engrafts better than counterpart. contrast,...
Using intravital imaging, we report that bone marrow (BM) plasma cells (PCs) are motile. BM PCs exhibit a unique migration pattern, characterized by intermittent periods of high motility and longer stretches confined or arrest. accumulate into clusters, which have reduced cell motility. APRIL promotes cluster formation overall PC in the BM. Although CXCL12 its receptor, CXCR4, promote BM, VLA4 activity However, blocking either pathway egress from Under steady-state conditions, recirculate to...
Abstract T cell recognition of peptide-MHC is highly specific and sensitive to very low levels agonist peptide; however, it unclear how this effect achieved or regulated. In study we show that clustering class I MHC molecules on the surface B lymphoblasts enhances their by mouse human cells. We increased two methods, cholesterol depletion direct cross-linking a dimerizable construct. Imaging showed both treatments size intensity clusters surface. Enlarged correlated with enhanced lysis...
Multiple myeloma (MM) is a plasma cell malignancy characterized by the presence of multiple foci in skeleton. These distinct tumor represent cycles growth and dissemination that seed new clusters drive disease progression. By using an intratibial Vk*MYC murine model, we found CD169+ radiation-resistant tissue-resident macrophages (MPs) were critical for early Depletion these MPs had no effect on proliferation, but it did reduce egress from bone marrow (BM) its spread to other bones. as...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTLigand Design Approach for Securing Robust Oxidation CatalystsColin P. Horwitz, David R. Fooksman, Leonard D. Vuocolo, Scott W. Gordon-Wylie, Nathaniel J. Cox, and Terrence CollinsView Author Information Department of Chemistry, Carnegie Mellon University 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213 Cite this: Am. Chem. Soc. 1998, 120, 19, 4867–4868Publication Date (Web):May 2, 1998Publication History Received18 August 1997Published...
Leukemia-driving mutations are thought to arise in hematopoietic stem cells (HSC), yet the natural history of their spread is poorly understood. We genetically induced within endogenous murine HSC and traced them unmanipulated animals. In contrast associated with clonal hematopoiesis (such as Tet2 deletion), leukemogenic KrasG12D mutation dramatically accelerated contribution all lineages. The acceleration was mediated by KrasG12D-expressing multipotent progenitors (MPP) that lacked...
T follicular helper (TFH) cells promote expansion of germinal center (GC) B and plasma cell differentiation. Whether cognate peptide-MHCII (pMHCII) density instructs selection fate decisions in a quantitative manner remains unclear. Using αDEC205-OVA to differentially deliver OVA peptides GC on the basis DEC205 allelic copy number, we find DEC205+/+ take up 2-fold more antigen than DEC205+/− cells, leading proportional TFH help expansion. To validate these results, establish caged peptide,...
Abstract Ab-secreting cell (ASC) expansion and survival are important processes in optimizing vaccines controlling autoimmunity. The microenvironment of the medullary cords is positioned to control these key processes. Previously, we imaged characterized ASC differentiation migration by intravital microscopy lymph node (LN) transferring activating B cells expressing yellow fluorescent protein only compartment. In this study, observed that protein+ ASCs migrated along myelomonocytic arrested...
Durable serological memory following vaccination is critically dependent on the production and survival of long-lived plasma cells (LLPCs). Yet, factors that control LLPC specification remain poorly resolved. Using intravital two-photon imaging, we find in contrast to most (PCs) bone marrow (BM), LLPCs are uniquely sessile organized into clusters APRIL, an important factor. deep, bulk RNA sequencing, surface protein flow-based phenotyping, express a unique transcriptome phenotype compared...