A5063852976- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Biochemical and Molecular Research
- Virology and Viral Diseases
- Immune responses and vaccinations
- Virus-based gene therapy research
- interferon and immune responses
- Animal Disease Management and Epidemiology
- Veterinary Oncology Research
- Salivary Gland Tumors Diagnosis and Treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Anesthesia and Pain Management
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- Head and Neck Anomalies
- Congenital Anomalies and Fetal Surgery
- Infectious Diseases and Tuberculosis
- Head and Neck Surgical Oncology
- Clostridium difficile and Clostridium perfringens research
- Hernia repair and management
- Mechanisms of cancer metastasis
- Advanced Drug Delivery Systems
- Enzyme Structure and Function
The University of Texas at El Paso
2020-2024
Chaudhary Charan Singh Haryana Agricultural University
2022
Sam Higginbottom Institute of Agriculture
2022
Dr Emilio B Espinosa Sr Memorial State College of Agriculture and Technology
2022
Albert Einstein College of Medicine
2013-2020
Gautam Buddha University
2019
Bharati Vidyapeeth Deemed University
2015
Howard Hughes Medical Institute
2014
Indian Veterinary Research Institute
2013
Command Hospital
2013
Immune interferon gamma (IFN-γ) is essential for mammalian host defense against intracellular pathogens. IFN-γ induces nearly 2000 genes, yet few have any assigned function. Here, we examined a complete mouse 65-kilodalton (kD) guanylate-binding protein (Gbp) gene family as part of 43-member IFN-γ-inducible guanosine triphosphatase (GTPase) superfamily in and human genomes. Family-wide loss-of-function analysis found that at least four Gbps--Gbp1, Gbp6, Gbp7, Gbp10--conferred cell-autonomous...
The role of lymphatic vessels is to transport fluid, soluble molecules, and immune cells the draining lymph nodes. Here, we analyze how aging process affects functionality collectors dynamics flow. Ultrastructural, biochemical, proteomic analysis indicates a loss matrix proteins, smooth muscle in aged resulting decrease contraction frequency, systolic flow velocity, pumping activity, as measured vivo collectors. Functionally, this impairment also translated into reduced ability for bacterial...
Reactive oxygen species (ROS)-mediated oxidative stress and DNA damage have recently been recognized as contributing to the efficacy of most bactericidal antibiotics, irrespective their primary macromolecular targets. Inhibitors targets involved in both combating well being required for vivo survival may exhibit powerful synergistic action. This study demonstrates that de novo arginine biosynthetic pathway Mycobacterium tuberculosis (Mtb) is up-regulated early response stress-elevating agent...
Mycobacterium tuberculosis bacilli display two signature features: acid-fast staining and the capacity to induce long-term latent infections in humans. However, mechanisms governing these important processes remain largely unknown. Ser/Thr phosphorylation has recently emerged as an regulatory mechanism allowing mycobacteria adapt their cell wall structure/composition response environment. Herein, we evaluated whether of KasB, a crucial mycolic acid biosynthetic enzyme, could modulate...
Spinal anaesthesia has been reported as an alternative to general for performing laparoscopic cholecystectomy (LC).Study aimed evaluate efficacy, safety and cost benefit of conducting under spinal (SA) in comparison anaesthesia(GA).A prospective, randomised study conducted over a two year period at urban, non teaching hospital.Patients meeting inclusion criteria e into groups. Group A B received by standardised techniques. Both groups underwent standard four port cholecystectomy. Mean time,...
The L-arginine biosynthesis pathway consists of eight enzymes that catalyse the conversion L-glutamate to L-arginine. Arginine auxotrophs (argB/argF deletion mutants) Mycobacterium tuberculosis are rapidly sterilised in mice, while inhibition ArgJ with Pranlukast was found clear chronic M. infection a mouse model. Enzymes arginine biosynthetic have therefore emerged as promising targets for anti-tuberculosis drug discovery. In this work, ligandability four ArgB, ArgC, ArgD and ArgF is...
The development of a new vaccine as substitute for Bacillus Calmette–Guerin or to improve its efficacy is one the many World Health Organization goals control tuberculosis. Mycobacterial vectors have been used successfully in vaccines against To enhance potential utility Mycobacterium smegmatis vaccine, it was transformed with recombinant plasmid containing partial sequences genes Ag85c, MPT51, and HspX (CMX) from M. newly generated strain mc2-CMX tested murine model infection. induced...
Nucleoside diphosphate kinase (NdK) is a ubiquitous enzyme in both prokaryotes and eukaryotes primarily involved the maintenance of cellular nucleotide pools. We have cloned ndk from Mycobacterium tuberculosis strain H37Ra expressed it Escherichia coli as fusion protein with glutathione S-transferase. The purified protein, following thrombin cleavage gel permeation chromatography, was found to be hexameric monomeric unit molecular mass ∼16.5 kDa. exhibited binding, divalent cation-dependent...
Aim: To assess the efficacy of internal sphincterotomy compared with application topical 2% Diltiazem ointment after hemorrhoidectomy for pain relief. Settings and Design: Prospective randomized study. Materials Methods: In an 18-month period, 108 subjects uncomplicated Grade 3/ 4 hemorrhoids were enrolled in study into two equal groups: Subjects Group A underwent at time primary surgery while those B received 1 g locally, thrice daily seven days. Postoperative perception was measured using...
Mycobacterium tuberculosis (Mtb), a successful human pathogen, resides in host sentinel cells and combats the stressful intracellular environment induced by reactive oxygen nitrogen species during infection. Mtb employs several evasion mechanisms face of as survival strategy, including detoxifying enzymes short-chain dehydrogenases/reductases (SDRs) to withstand host-generated insults. In this study, using specialized transduction, we have generated Rv0687 deletion mutant its complemented...