A5086359802- Tuberculosis Research and Epidemiology
- Salmonella and Campylobacter epidemiology
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- Vibrio bacteria research studies
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Influenza Virus Research Studies
- Erythrocyte Function and Pathophysiology
- Bacterial Genetics and Biotechnology
- French Urban and Social Studies
- Psychoanalysis and Psychopathology Research
- Aging, Elder Care, and Social Issues
- Lipid Membrane Structure and Behavior
- Zebrafish Biomedical Research Applications
- Cellular transport and secretion
- Protist diversity and phylogeny
- PI3K/AKT/mTOR signaling in cancer
- Cellular Mechanics and Interactions
- Protein Structure and Dynamics
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
Centre National de la Recherche Scientifique
2010-2024
Université de Montpellier
2012-2024
Inserm
2011-2024
University of Geneva
2016-2021
Laboratory of Pathogens and Host Immunity
2011-2014
Mansfield University
2012
Agruicultural Research Institute
2011
Université Claude Bernard Lyon 1
2010
Structure Fédérative de Recherche Biosciences
2010
Institut de Biologie et de Chimie des Protéines
2010
Mycobacterium tuberculosis bacilli display two signature features: acid-fast staining and the capacity to induce long-term latent infections in humans. However, mechanisms governing these important processes remain largely unknown. Ser/Thr phosphorylation has recently emerged as an regulatory mechanism allowing mycobacteria adapt their cell wall structure/composition response environment. Herein, we evaluated whether of KasB, a crucial mycolic acid biosynthetic enzyme, could modulate...
Numerous intracellular bacterial pathogens interfere with macrophage function, including polarization, to establish a niche and persist. However, the spatiotemporal dynamics of polarization during infection within host remain be investigated. Here, we implement model persistent Salmonella Typhimurium in zebrafish, which allows visualization polarized macrophages bacteria real time at high resolution. While polarize toward M1-like phenotype control early infection, later stages, persists...
One major signaling method employed by Mycobacterium tuberculosis, the causative agent of is through reversible phosphorylation proteins mediated protein kinases and phosphatases. This study concerns one these enzymes, serine/threonine kinase PknF, that encoded in an operon with Rv1747, ABC transporter necessary for growth M. tuberculosis vivo contains two forkhead-associated (FHA) domains. FHA domains are phosphopeptide recognition motifs specifically recognize phosphothreonine-containing...
Bacterial sensing, ingestion, and killing by phagocytic cells are essential processes to protect the human body from infectious microorganisms. The cellular mechanisms involved in intracellular killing, their relative importance, specificity towards different bacteria however poorly defined. In this study, we used Dictyostelium discoideum, a cell model amenable genetic analysis, identify new gene products killing. A random screen led us role of Vps13F Klebsiella pneumoniae. knock-out (KO)...
Corynebacteria grow by wall extension at the cell poles, with DivIVA being an essential protein orchestrating elongation and morphogenesis. is considered a scaffolding able to recruit other proteins enzymes involved in polar peptidoglycan biosynthesis. Partial depletion of induced overexpression cg3264, previously uncharacterized gene that encodes novel coiled coil-rich specific for corynebacteria few actinomycetes. By partial Cg3264, we demonstrated this cytoskeletal element needed...
Identification of the phosphorylated residues bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present new strategy allowing rapid determination phosphoacceptors in substrates, essentially based on dual expression with its substrate surrogate E. coli, followed by MS analysis single-step procedure. The performance this is illustrated using two distinct proteins from Mycobacterium tuberculosis as model GroEL2 and HspX chaperones. A comparative...
Phagocytic cells ingest bacteria by phagocytosis and kill them efficiently inside phagolysosomes. The molecular mechanisms involved in intracellular killing their regulation are complex still incompletely understood. Dictyostelium discoideum has been used as a model to discover study new gene products of ingested bacteria. In this study, we performed random mutagenesis isolated mutant defective for growth on This is characterized the genetic inactivation lrrkA gene, which encodes protein...
Dictyostelium discoideum has been used largely as a model organism to study the organization and function of endocytic pathway. Here we describe dense structures present in D. compartments, which named pycnosomes. Pycnosomes are constitutively secreted extracellular medium, from they can be recovered by differential centrifugation. We identified most abundant protein pycnosomes, that designated SctA. SctA defines new family proteins with four members discoideum, homologous other protists...
LrrkA is a Dictyostelium discoideum kinase with leucine-rich repeats. stimulates Kil2 and intra-phagosomal killing of ingested bacteria in response to folate. In this study, we show that genetic inactivation lrrkA also causes previously unnoticed phenotype: KO cells exhibit enhanced phagocytosis cell motility compared parental cells. This phenotype autonomous, reversible upon re-expression LrrkA, not due an abnormal inhibitory quorum-sensing factors secreted by D. its medium. addition,...
ABSTRACT Numerous intracellular bacterial pathogens interfere with macrophage function, including polarization, to establish a niche and persist. However, the spatiotemporal dynamics of polarization during infection within host remain be investigated. Here, we implement model persistent Salmonella Typhimurium in zebrafish, which allows visualization polarized macrophages bacteria real time at high-resolution. While polarize toward M1-like phenotype control early infection, later stages,...