A5051926579- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Micro and Nano Robotics
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- Adhesion, Friction, and Surface Interactions
- Surface Modification and Superhydrophobicity
- Microtubule and mitosis dynamics
- Molecular Communication and Nanonetworks
- stochastic dynamics and bifurcation
- Cancer Cells and Metastasis
- Nanopore and Nanochannel Transport Studies
- Biocrusts and Microbial Ecology
- Slime Mold and Myxomycetes Research
- Mathematical Biology Tumor Growth
- Cell Adhesion Molecules Research
- Electrowetting and Microfluidic Technologies
- Gene Regulatory Network Analysis
- Mechanical and Optical Resonators
- Planarian Biology and Electrostimulation
- Pickering emulsions and particle stabilization
- Electrostatics and Colloid Interactions
- Advanced biosensing and bioanalysis techniques
- Polymer Surface Interaction Studies
- Bone Tissue Engineering Materials
Institut Curie
2016-2025
Physique des Cellules et Cancers
2013-2024
Sorbonne Université
2014-2024
Université Paris Sciences et Lettres
2016-2024
Centre National de la Recherche Scientifique
2012-2023
Leiden University
2020
Délégation Paris 6
2018
Université Paris Cité
2006-2010
Délégation Paris 7
2010
Laboratoire Matière et Systèmes Complexes
2006-2010
Using an original microfabrication-based technique, we experimentally study situations in which a virgin surface is presented to confluent epithelium with no damage made the cells. Although inspired by wound-healing experiments, situation markedly different from classical scratch wounding because it focuses on influence of free and uncouples other possible contributions such as cell and/or permeabilization. Dealing Madin-Darby canine kidney cells various surfaces, found that sudden release...
When a liquid drops impinges hydrophobic rough surface it can either bounce off the (fakir droplets) or be impaled and strongly stuck on (Wenzel droplets). The analysis of drop impact quasi static ''loading'' experiments model microfabricated surfaces allows to clearly identify forces hindering impalement transitions. A simple semi-quantitative is proposed account for observed relation between topography robustness fakir non-wetting states. Motivated by potential applications in...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSilanation of silica surfaces. A new method constructing pure or mixed monolayersP. Silberzan, L. Leger, D. Ausserre, and J. BenattarCite this: Langmuir 1991, 7, 8, 1647–1651Publication Date (Print):August 1, 1991Publication History Published online1 May 2002Published inissue 1 August 1991https://pubs.acs.org/doi/10.1021/la00056a017https://doi.org/10.1021/la00056a017research-articleACS PublicationsRequest reuse permissionsArticle...
We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel scales linearly with contour length of polymer, agreement scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly single channels, and statistical analysis dynamics polymer nanochannel compute SD mean extension. λ multimers a 130-nm 1 min.
Significance Collective cell motion is very important in many biological processes such as wound healing, embryogenesis, or cancer progression. Nevertheless, it not clear which parameters control the transition from freely moving single cells to collective jammed motion. In this article, we uncover complex dynamics a monolayer ages, where shown gradually slow down with time, while distance over displacements are correlated first increases drastically and then decreases. This change of...
Abstract Activity and autonomous motion are fundamental in living engineering systems. This has stimulated the new field of ‘active matter’ recent years, which focuses on physical aspects propulsion mechanisms, motility-induced emergent collective behavior a larger number identical agents. The scale agents ranges from nanomotors microswimmers, to cells, fish, birds, people. Inspired by biological various designs synthetic nano- micromachines have been proposed. Such machines provide basis...
Increasing evidence suggests that mechanical cues inherent to the extracellular matrix may be as important its chemical nature in regulating cell behavior. Here, response of cells properties substrate is examined by culturing 3T3 fibroblastic and epithelial on surfaces composed a dense array flexible microfabricated pillars. We focus influence rigidity traction forces exerted cells, adhesion migration. first measure these monitoring deflection Then, varying their geometric parameters, we...
The physical properties of the cellular environment are involved in regulating formation and maintenance tissues. In particular, substrate rigidity appears to be a key factor dictating cell response on culture surfaces. Here we study behavior epithelial cells cultured microfabricated substrates engineered exhibit an anisotropic stiffness. consists dense array micropillars oval cross-section, so that one direction is made stiffer than other. We demonstrate how such can induce directional...
The production, via soft lithography, of micron-sized thermoresponsive pillars made side-on nematic LCE leads to the formation surface-responsive structures. individual, monodisperse pillars, when cut out from surface, behave as micro-actuators, showing contraction around 35% at isotropic phase transition.
Although fibroblast heterogeneity is recognized in primary tumors, both its characterization and impact on metastases remain unknown. Here, combining flow cytometry, immunohistochemistry RNA-sequencing breast cancer samples, we identify four Cancer-Associated Fibroblast (CAF) subpopulations metastatic lymph nodes (LN). Two myofibroblastic subsets, CAF-S1 CAF-S4, accumulate LN correlate with cell invasion. By developing functional assays cultures, demonstrate that these subsets promote...
Modelling the displacement of thousands cells that move in a collective way is required for simulation and theoretical analysis various biological processes. Here, we tackle this question controlled setting where motion Madin-Darby Canine Kidney (MDCK) confluent epithelium triggered by unmasking free surface. We develop simple model which are described as point particles with dynamic based on two premises that, first, stochastic manner and, second, tend to adapt their neighbors. Detailed...
Cell competition is a quality control mechanism that eliminates unfit cells. How cells compete poorly understood, but it generally accepted molecular exchange between signals elimination of Here we report an orthogonal cell competition, whereby through mechanical insults. We show MDCK silenced for the polarity gene scribble (scribKD) are hypersensitive to compaction, interaction with wild-type causes their compaction and crowding sufficient scribKD elimination. Importantly, elevation tumour...
Chemotactic bacteria are known to collectively migrate towards sources of attractants. In confined convectionless geometries, concentration “waves” swimming Escherichia coli can form and propagate through a self-organized process involving hundreds thousands these microorganisms. These waves observed in particular microcapillaries or microchannels; they result from the interaction between individual chemotactic macroscopic chemical gradients dynamically generated by migrating population. By...
Mechanical forces play an important role in the organization, growth and function of living tissues. The ability cells to transduce mechanical signals is governed by two types microscale structures: focal adhesions, which link extracellular matrix, adherens junctions, adjacent through cadherins. Although many studies have examined induced there little known about junctions force-regulation processes. present study focuses on determination force transduction cadherins at a single cell level....
Elongated, weakly interacting, apolar, fibroblast cells (mouse fibroblasts NIH-3T3) cultured at confluence align together, forming large domains (correlation length ∼ 500 μm) where they are perfectly ordered. We study the emergence of this mesoscopic nematic order by quantifying ordering dynamics in a two-dimensional tissue. Cells initially very motile and monolayer is characterized anomalous density fluctuations, signature far-from-equilibrium systems. As cell increases because...
The environmental topology of complex structures is used by Escherichia coli to create traveling waves high cell density, a prelude quorum sensing. When cells are grown moderate density within confining microenvironment, these allow the find and collapse into topologies, which unstable population fluctuations above critical threshold. This was first observed in mazes designed mimic environments, then more clearly simpler geometry consisting large open area surrounding square (250 × 250 μm)...