A5010656717- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Biosensors and Analytical Detection
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Chromatography
- Neuroscience and Neural Engineering
- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Cancer Genomics and Diagnostics
- Electrohydrodynamics and Fluid Dynamics
- Cell Image Analysis Techniques
- Hippo pathway signaling and YAP/TAZ
- Single-cell and spatial transcriptomics
- Muscle activation and electromyography studies
- Analytical Chemistry and Sensors
- Pregnancy and preeclampsia studies
- Nanofabrication and Lithography Techniques
- Tissue Engineering and Regenerative Medicine
- Advanced Electron Microscopy Techniques and Applications
- Electrochemical sensors and biosensors
Centre National de la Recherche Scientifique
2016-2025
Université Paris Sciences et Lettres
2016-2025
Institut Pierre-Gilles de Gennes pour la Microfluidique
2016-2025
Institut Curie
2016-2025
Sorbonne Université
2015-2025
Physique des Cellules et Cancers
2015-2024
Chimie ParisTech
2001-2024
École Normale Supérieure - PSL
2010-2024
Institut Pprime
2022-2023
Google (United States)
2022
Although fibroblast heterogeneity is recognized in primary tumors, both its characterization and impact on metastases remain unknown. Here, combining flow cytometry, immunohistochemistry RNA-sequencing breast cancer samples, we identify four Cancer-Associated Fibroblast (CAF) subpopulations metastatic lymph nodes (LN). Two myofibroblastic subsets, CAF-S1 CAF-S4, accumulate LN correlate with cell invasion. By developing functional assays cultures, demonstrate that these subsets promote...
Abstract During tumor progression, cancer-associated fibroblasts (CAFs) accumulate in tumors and produce an excessive extracellular matrix (ECM), forming a capsule that enwraps cancer cells. This acts as barrier restricts growth leading to the buildup of intratumoral pressure. Combining genetic physical manipulations vivo with microfabrication force measurements vitro, we found CAFs is not passive but instead actively compresses cells using actomyosin contractility. Abrogation contractility...
Stem cells perceive and respond to biochemical physical signals maintain homeostasis. Yet, it remains unclear how stem sense mechanical from their niche in vivo. In this work, we investigated the roles of PIEZO mechanosensitive channels intestinal cell (ISC) niche. We used mouse genetics single-cell RNA sequencing analysis assess requirement for ISC maintenance. vivo measurement basement membrane stiffness showed that ISCs reside a more rigid microenvironment at bottom crypt....
A major challenge in cancer research is the complexity of tumor microenvironment, which includes host immunological setting. Inspired by emerging technology organ-on-chip, we achieved 3D co-cultures microfluidic devices (integrating four cell populations: cancer, immune, endothelial, and fibroblasts) to reconstitute ex vivo a human ecosystem (HER2+ breast cancer). We visualized quantified complex dynamics this tumor-on-chip, absence or presence drug trastuzumab (Herceptin), targeted antibody...
There is a compelling need for approaches to predict the efficacy of immunotherapy drugs. Tumor-on-chip technology exploits microfluidics generate 3D cell co-cultures embedded in hydrogels that recapitulate simplified tumor ecosystems. Here, we present development and validation lung tumor-on-chip platforms quickly precisely measure ex vivo effects immune checkpoint inhibitors on T cell-mediated cancer death by exploiting power live imaging advanced image analysis algorithms. The integration...
In this paper we report the combination of microfluidics, optogenetics and calcium imaging as a cheap convenient platform to study synaptic communication between neuronal populations in vitro. We first show that Calcium Orange indicator is compatible vitro with commonly used Channelrhodopsine-2 (ChR2) variant, standard conditions did not alter significantly activity transduced cultures rodent primary neurons. A fast, robust scalable process for micro-chip fabrication was developed parallel...
We report on a new gut chip combining the co-culture of primary epithelial and stromal cells in 3D biomimetic scaffold. Proper segregation dividing differentiated along crypt-villus axis was achieved these unique conditions.
A new generation of the Ephesia cell capture technology optimized for CTC and genetic analysis is presented, characterized in depth compared with CellSearch system as a reference.
A microfluidic fluidized bed composed of antibody-grafted magnetic beads for the fast visual detection bacteria by <italic>in situ</italic> expansion phenomena.
Tweezing out the answer: A microfluidic device combining droplets (less than 100 nL) and magnetic particles (see scheme) was implemented for fast heterogeneous multiplexed assays. Magnetic tweezers can perform manipulations required in an immunoassay (capture, extraction, mixing, rinsing). This method applied to diagnosis of congenital hypothyroidism with 14 pM sensitivity. Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed,...
We report on the development of a simple and easy to use microchip dedicated allergy diagnosis. This combines both advantages homogeneous immunoassays i.e. species diffusion heterogeneous separation preconcentration steps. In vitro diagnosis is based specific Immunoglobulin E (IgE) quantitation, in that way we have developed integrated magnetic core-shell nanoparticles (MCSNPs) as an IgE capture nanoplatform microdevice taking benefit from their colloidal properties. Integrating such...
Abstract The development of precision medicine, together with the multiplication targeted therapies and associated molecular biomarkers, call for major progress in genetic analysis methods, allowing increased multiplexing implementation more complex decision trees, without cost increase or loss robustness. We present a platform combining droplet microfluidics magnetic tweezers, performing RNA purification, reverse transcription amplification fully automated programmable way, droplets 250nL...
An adhesive-based strategy for the low-cost and reversible sealing of a wide range materials used in microfluidics, requiring only application manually-achievable pressures.
Tunnelling nanotubes (TNTs) connect distant cells and mediate cargo transfer for intercellular communication in physiological pathological contexts. How generate these actin-mediated protrusions to span lengths beyond those attainable by canonical filopodia remains unknown. Through a combination of micropatterning, microscopy, optical tweezer-based approaches, we demonstrate that TNTs formed through the outward extension actin achieve distances greater than mean length branched...
In this work, we propose a novel strategy for the fabrication of flexible thermoplastic microdevices entirely based on lamination processes. The same low-cost laminator apparatus can be used from master to microchannel sealing. This process is appropriate rapid prototyping at laboratory scale, but it also easily upscaled industrial manufacturing. For demonstration, here Cycloolefin Copolymer (COC), polymer that extensively microfluidic applications. COC with good chemical resistance common...
We present an integrated microfluidic chip for detection of β-amyloid (Aβ) peptides. Aβ peptides are major biomarkers the diagnosis Alzheimer's disease (AD) in its early stages. This device consists three main parts: (1) An immunocapture microcolumn based on self-assembled magnetic beads coated with antibodies specific to peptides, (2) a nano-porous membrane made photopolymerized hydrogel preconcentration, and (3) microchip electrophoresis (MCE) channel fluorescent detection. Sub-milliliter...
Fluidization of micromagnetic beads in microfluidics for flow-through bioanalytical operations at low driving pressures, high clogging resistance and favorable binding kinetics.