A5012437943- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Renal and related cancers
Institut Gustave Roussy
2021-2024
Inserm
2022-2024
Immunologie intégrative des tumeurs et immunothérapie du cancer
2021-2024
Laboratoire d’immunologie intégrative du cancer
2021-2024
Université Paris-Saclay
2022-2023
Université Paris-Sud
2022-2023
Abstract TGF-β is secreted in the tumour microenvironment a latent, inactive form bound to latency associated protein and activated by integrin α V subunit. The activation of latent cancer-cell-expressed re-shapes microenvironment, this could affect patient responses PD-1-targeting therapy. Here we show, using multiplex immunofluorescence staining cohorts anti-PD-1 anti-PD-L1-treated lung cancer patients, that decreased expression cell with improved immunotherapy-related, progression-free...
Background Cancer stem cells (CSC) define a population of rare malignant endowed with ‘stemness’ properties, such as self-renewing, multipotency and tumorigenicity. They are responsible for tumor initiation progression, could be associated resistance to immunotherapies by negatively regulating antitumor immune response acquiring molecular features enabling escape from CD8 T-cell immunity. However, the immunological hallmarks human lung CSC their potential interactions resident memory T (T RM...
There is a compelling need for approaches to predict the efficacy of immunotherapy drugs. Tumor-on-chip technology exploits microfluidics generate 3D cell co-cultures embedded in hydrogels that recapitulate simplified tumor ecosystems. Here, we present development and validation lung tumor-on-chip platforms quickly precisely measure ex vivo effects immune checkpoint inhibitors on T cell-mediated cancer death by exploiting power live imaging advanced image analysis algorithms. The integration...
von Hippel-Lindau (VHL) disease, due to mutations of the tumor suppressor VHL gene, is a rare hereditary syndrome with high risk developing clear cell renal carcinoma (ccRCC). We asked whether VHL-C162F mutation interferes proliferation, migration, healing and forming colony ability by using wild-type (WT VHL) reconstituted cells. then analyzed in vitro impact sunitinib treatment on showed that have no morphology, formation migration but confer significant higher than WT RNA sequencing...
Abstract CD8+ resident memory T (TRM) cells expressing the CD103 integrin accumulate in human lung tumors and are associated with a favorable prognosis. We have previously demonstrated that high density of CD103+CD8+ is biomarker response to immune checkpoint blockade (ICB) immunotherapy. However, large fraction only weakly infiltrated by TRM cells, reason this desert not explained. Therefore, defining molecular signals give rise maintain within tumor microenvironment (TME) mechanisms...
Abstract CD8+CD103+ TRM cells accumulate in human lung tumors and are associated with a favorable prognosis response to immune checkpoint blockade (ICB). However, large fraction of solid is only weakly infiltrated by TRM, the reason this desert not explained. Defining molecular signals that give rise maintain within tumor microenvironment (TME) mechanisms potentiate antitumor functions important challenges. Data obtained pre-clinical mouse models demonstrate knocking-out CD103 impairs T-cell...
ABSTRACT There is a compelling need for new approaches to predict efficacy of immunotherapy drugs. Tumor-on-chip technology exploits microfluidics generate 3D cell co-cultures embedded in hydrogels that recapitulate immune and stromal characteristics simplified tumor ecosystem. Here, we present the development validation lung-tumor-on-chip platforms quickly precisely measure ex vivo effects check-point inhibitors on T-cell-mediated cancer death, by exploiting power live imaging advanced...