A5063922043- Protein Kinase Regulation and GTPase Signaling
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Advanced Fluorescence Microscopy Techniques
- Image Processing Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cellular transport and secretion
- Cell death mechanisms and regulation
- Single-cell and spatial transcriptomics
- Hippo pathway signaling and YAP/TAZ
- Advanced Vision and Imaging
- Autophagy in Disease and Therapy
- Microfluidic and Bio-sensing Technologies
- Cell Adhesion Molecules Research
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Protein Tyrosine Phosphatases
- PI3K/AKT/mTOR signaling in cancer
- Endoplasmic Reticulum Stress and Disease
- Cancer-related Molecular Pathways
- Photoacoustic and Ultrasonic Imaging
- Functional Brain Connectivity Studies
Université Paris Sciences et Lettres
2016-2025
Inserm
2016-2025
Institut Curie
2016-2025
Centre National de la Recherche Scientifique
1994-2025
La Ligue Contre le Cancer
2022
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2021
Sorbonne Université
2018
Directorate-General Joint Research Centre
2012-2016
Boston Children's Hospital
1999-2000
Harvard University
1999-2000
Although fibroblast heterogeneity is recognized in primary tumors, both its characterization and impact on metastases remain unknown. Here, combining flow cytometry, immunohistochemistry RNA-sequencing breast cancer samples, we identify four Cancer-Associated Fibroblast (CAF) subpopulations metastatic lymph nodes (LN). Two myofibroblastic subsets, CAF-S1 CAF-S4, accumulate LN correlate with cell invasion. By developing functional assays cultures, demonstrate that these subsets promote...
A major challenge in cancer research is the complexity of tumor microenvironment, which includes host immunological setting. Inspired by emerging technology organ-on-chip, we achieved 3D co-cultures microfluidic devices (integrating four cell populations: cancer, immune, endothelial, and fibroblasts) to reconstitute ex vivo a human ecosystem (HER2+ breast cancer). We visualized quantified complex dynamics this tumor-on-chip, absence or presence drug trastuzumab (Herceptin), targeted antibody...
Abstract We describe a novel method to achieve universal, massive, and fully automated analysis of cell motility behaviours, starting from time-lapse microscopy images. The approach was inspired by the recent successes in application machine learning for style recognition paintings artistic transfer. originality relies i) on generation atlas collection single-cell trajectories order visually encode multiple descriptors motility, ii) pre-trained Deep Learning Convolutional Neural Network...
There is a compelling need for approaches to predict the efficacy of immunotherapy drugs. Tumor-on-chip technology exploits microfluidics generate 3D cell co-cultures embedded in hydrogels that recapitulate simplified tumor ecosystems. Here, we present development and validation lung tumor-on-chip platforms quickly precisely measure ex vivo effects immune checkpoint inhibitors on T cell-mediated cancer death by exploiting power live imaging advanced image analysis algorithms. The integration...
The two Ral GTPases, RalA and RalB, have crucial roles downstream Ras oncoproteins in human cancers; particular, RalB is involved invasion metastasis. However, therapies targeting signalling are not available yet. By a novel optogenetic approach, we found that light-controlled activation of at plasma-membrane promotes the recruitment Wave Regulatory Complex (WRC) via its effector exocyst, with consequent induction protrusions invasion. We show active signals to RalGEFs (Guanine nucleotide...
Abstract Cell-cell interactions are an observable manifestation of underlying complex biological processes occurring in response to diversified biochemical stimuli. Recent experiments with microfluidic devices and live cell imaging show that it is possible characterize kinematics via computerized algorithms unravel the effects targeted therapies. We study influence spatial temporal resolutions time-lapse videos on motility interaction descriptors computational models mimic dynamics among...
One of the major problems in bioimaging, often highly underestimated, is whether features extracted for a discrimination or regression task will remain valid broader set similar experiments presence unpredictable perturbations during image acquisition process. Such an issue even more important when it addressed context deep learning due to lack priori known relationship between black-box descriptors (deep features) and phenotypic properties biological entities under study. In this regard,...
Live-cell microscopy routinely provides massive amounts of time-lapse images complex cellular systems under various physiological or therapeutic conditions. However, this wealth data remains difficult to interpret in terms causal effects. Here, we describe CausalXtract, a flexible computational pipeline that discovers and possibly time-lagged effects from morphodynamic features cell–cell interactions live-cell imaging data. CausalXtract methodology combines network-based information-based...
The p21-activated kinase (PAK) 1 kinase, an effector of the Cdc42 and Rac1 GTPases, regulates cell protrusions motility by controlling actin adhesion dynamics. Its deregulation has been linked to human cancer. We show here that activation PAK1 is necessary for protrusive activity during spreading. To investigate dynamics at live protrusions, we developed a conformational biosensor, based on fluorescence resonance energy transfer. This novel biosensor allowed spatiotemporal visualization...
Abstract RalA and RalB proteins are key mediators of oncogenic Ras signaling in human oncogenesis. Herein we investigated the mechanistic contribution Ral to invasion lung cancer A549 cells after induction epithelial-mesenchymal transition (EMT) with TGFβ. We show that TGFβ-induced EMT promotes dissemination a 2/3D assay, independently proteolysis, by activating Rho/ROCK pathway which generates actomyosin-dependent contractility forces actively remodel extracellular matrix, as assessed...
Abstract The endothelium compartment is a key player in tumor initiation and progression, but most existing tumor‐on‐chip models lack clinical relevance. Here, 3D vascularized (vToC) model, generated with patient‐derived microvascular endothelial cells (ECs) that are freshly isolated from surgical lung cancer samples, presented. microvessel molecular identity, morphology, functionality assessed by transcriptomic, immunofluorescence, TNF‐α stimulation, permeability assays. Lung cells,...