A5049362015- Psychoanalysis and Psychopathology Research
- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Nuclear Structure and Function
- Developmental Biology and Gene Regulation
- Autophagy in Disease and Therapy
- Protein Kinase Regulation and GTPase Signaling
- Genomics and Chromatin Dynamics
- Biotin and Related Studies
- French Literature and Critical Theory
- Cellular transport and secretion
- Pluripotent Stem Cells Research
- Political and Social Issues
- Melanoma and MAPK Pathways
- Wnt/β-catenin signaling in development and cancer
- Educational Philosophies and Pedagogies
- Tissue Engineering and Regenerative Medicine
- Signaling Pathways in Disease
- Cell death mechanisms and regulation
- Death, Funerary Practices, and Mourning
- Cannabis and Cannabinoid Research
- Medical and Biological Sciences
- Philosophy and Social Theory
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
Harvard University
2020-2024
Boston VA Research Institute
2020-2024
Université Bordeaux Montaigne
2021
Aix-Marseille Université
2020
Institut Curie
2019
Inserm
2019
University of Saskatchewan
2012
Notch signaling relies on ligand-induced proteolysis of the transmembrane receptor to liberate a nuclear effector that drives cell fate decisions. Upon ligand binding, sequential cleavage by protease ADAM10 and intracellular γ-secretase releases domain (NICD), which translocates nucleus forms complex induces target gene transcription. To map location timing individual steps required for movement from plasma membrane nucleus, we used proximity labeling with quantitative, multiplexed mass...
RASSF1A, a tumor suppressor gene, is frequently inactivated in lung cancer leading to YAP-dependent epithelial-mesenchymal transition (EMT). Such effects are partly due the inactivation of anti-migratory RhoB GTPase via inhibitory phosphorylation GEF-H1, GDP/GTP exchange factor for RhoB. However, kinase responsible RhoB/GEF-H1 RASSF1A-depleted cells remained unknown. NDR1/2 by siRNA or shRNA on transition, invasion, xenograft formation and growth SCID-/- Beige mice, apoptosis, proliferation,...
Breast tumor kinase (BRK) is a non-receptor tyrosine overexpressed in most human breast tumors, including lymph node metastases, but undetected normal mammary tissue or fibroadenomas. The activity of BRK-like Src family kinase, regulated negatively by phosphorylation C-terminal 447. Although the that regulates BRK activation has not been identified, we and others have previously shown BRK-Y447F constitutively active variant. Because significantly enhances catalytic enzyme, investigated role...
Notch proteins undergo ligand-induced proteolysis to release a nuclear effector that influences wide range of cellular processes by regulating transcription. Despite years study, however, how induces the transcription its target genes remains unclear. Here, we comprehensively examine response human Notch1 across time course activation using high-resolution genomic assays chromatin accessibility and nascent RNA production. Our data reveal gene primarily releasing paused polymerase II...
The monomeric GTPase RalB controls crucial physiological processes, including autophagy and invasion, but it still remains unclear how this multi-functionality is achieved. Previously, we reported that the RalGEF (Guanine nucleotide Exchange Factor) RGL2 binds activates to promote invasion. Here show RGL2, a major activator of RalB, also required for autophagy. Using novel automated image analysis method, Endomapper, quantified endogenous localization its substrate at different endomembrane...
Notch proteins undergo ligand-induced proteolysis to release a nuclear effector that influences wide range of cellular processes by regulating transcription. Despite years study, however, how induces the transcription its target genes remains unclear. Here, we comprehensively examined response human Notch1 across time course activation using high-resolution genomic assays chromatin accessibility and nascent RNA production. Our data reveal gene primarily releasing paused polymerase II...
Abstract Mammalian Notch signaling occurs when binding of Delta or Jagged to stimulates proteolytic release the intracellular domain (NICD), which enters nucleus regulate target gene expression. To determine temporal dynamics events associated with under native conditions, we fluorescently tagged and at their endogenous genomic loci visualized them upon pairing receiver (Notch) sender (Delta) cells as a function time after cell contact. At contact sites, immediately accumulated 1:1...
Le camp constitue l’une des institutions centrales du régime totalitaire nazi où, comme le dit Hannah Arendt, « tout devient possible ». En s’attaquant à ce qui se trouve au fondement de la subjectivité et l’intersubjectivité humaine, il s’agit détruire l’action. Toutefois, qu’a mis en lumière littérature concentrationnaire, c’est que vient heurter un reste indestructible : confiance primordiale foyer toutes les modalités confiance.
ABSTRACT Notch signaling relies on ligand-induced proteolysis to liberate a nuclear effector that drives cell fate decisions. The location and timing of individual steps required for movement from membrane nucleus, however, remain unclear. Here, we use proximity labeling with quantitative multiplexed mass spectrometry monitor the microenvironment endogenous Notch2 after ligand stimulation in presence gamma secretase inhibitor then as function time removal. Our studies show cleavage occurs an...
Le camp constitue l’une des institutions centrales du régime totalitaire nazi où, comme le dit Hannah Arendt, « tout devient possible ». En s’attaquant à ce qui se trouve au fondement de la subjectivité et l’intersubjectivité humaine, il s’agit détruire l’action. Toutefois, qu’a mis en lumière littérature concentrationnaire, c’est que vient heurter un reste indestructible : confiance primordiale foyer toutes les modalités confiance.
La méthode généalogique de Nietzsche interroge la guerre du point vue des pulsions qu’elle mobilise. En ce sens elle met à l’épreuve le type d’homme qui pratique. C’est pourquoi une lecture probe et rigoureuse son œuvre, se situe par-delà dévoiement nazi, doit nous amener réfléchir manière dont guerre, si l’on sait choisir, est créatrice valeurs, suppose l’exercice l’appropriation certaines vertus.