A5075947402- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Electrical and Bioimpedance Tomography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Immune Cell Function and Interaction
- Electrowetting and Microfluidic Technologies
- Cellular Mechanics and Interactions
- Immunotherapy and Immune Responses
- Surface Modification and Superhydrophobicity
- Spectroscopy and Chemometric Analyses
- Single-cell and spatial transcriptomics
- IL-33, ST2, and ILC Pathways
- Biosensors and Analytical Detection
- Immune cells in cancer
- Nanofabrication and Lithography Techniques
- Adhesion, Friction, and Surface Interactions
- CAR-T cell therapy research
- Plasmonic and Surface Plasmon Research
- Cancer Immunotherapy and Biomarkers
- Neuroblastoma Research and Treatments
- Bacterial biofilms and quorum sensing
- Mathematical Biology Tumor Growth
- Mycotoxins in Agriculture and Food
National Research Council
2016-2025
Center for Biomolecular Nanotechnologies
2014-2025
Istituto di Fotonica e Nanotecnologie
2013-2023
National Research Council
2021
University of Rome Tor Vergata
2017-2020
National Academies of Sciences, Engineering, and Medicine
2012-2020
Consorzio Roma Ricerche
2016
Università Campus Bio-Medico
2014
Sapienza University of Rome
2013
How dying tumor cells get noticed Besides killing directly, some chemotherapies, such as anthracyclines, also activate the immune system to kill tumors. Vacchelli et al. discovered that in mice, anthracycline-induced antitumor immunity requires express protein formyl peptide receptor 1 (FPR1). Dendritic (DCs) near tumors expressed especially high amounts of FPR1. DCs normally capture fragments and use them nearby T tumors, but lacking FPR1 failed do this effectively. Individuals with breast...
Cancer stem cells (CSCs) are a subpopulation of cancer endowed with high tumorigenic, chemoresistant and metastatic potential. Nongenetic mechanisms acquired resistance increasingly being discovered, but molecular insights into the evolutionary process CSCs limited. Here, we show that type I interferons (IFNs-I) function as hubs during immunogenic chemotherapy, triggering epigenetic regulator demethylase 1B (KDM1B) to promote an adaptive, yet reversible, transcriptional rewiring towards...
Immunotherapy efficacy relies on the crosstalk within tumor microenvironment between cancer and dendritic cells (DCs) resulting in induction of a potent effective antitumor response. DCs have specific role recognizing cells, taking up antigens (Ags) then migrating to lymph nodes for Ag (cross)-presentation naïve T cells. Interferon-α-conditioned (IFN-DCs) exhibit marked phagocytic activity special ability inducing Ag-specific T-cell Here, we developed novel microfluidic platform recreating...
A major challenge in cancer research is the complexity of tumor microenvironment, which includes host immunological setting. Inspired by emerging technology organ-on-chip, we achieved 3D co-cultures microfluidic devices (integrating four cell populations: cancer, immune, endothelial, and fibroblasts) to reconstitute ex vivo a human ecosystem (HER2+ breast cancer). We visualized quantified complex dynamics this tumor-on-chip, absence or presence drug trastuzumab (Herceptin), targeted antibody...
The reconstitution of a complex microenvironment on microfluidic chips is one the cornerstones to demonstrate improved flexibility these devices with respect macroscale in vitro approaches. In this work, we realised an on-chip model investigate interactions between cancer and immune system. To end, exploited mice deficient (Knock Out, KO) for interferon regulatory factor 8 (IRF-8), transcription essential induction competent responses, how IRF-8 gene expression contributes regulate melanoma...
At its most fundamental level, touch sensation requires the translation of mechanical energy into mechanosensitive ion channel opening, thereby generating electro-chemical signals. Our understanding this process, especially how cytoskeleton influences it, remains unknown. Here we demonstrate that mice lacking α-tubulin acetyltransferase Atat1 in sensory neurons display profound deficits their ability to detect stimuli. We show all cutaneous afferent subtypes, including nociceptors have...
A successful outcome of the coupling between microfluidics and AI: neural networks tackle signal processing challenges single-cell microfluidic impedance cytometry.
We present an original five-electrode coplanar chip enabling accurate particle sizing without the need for focusing.
In this paper we discuss the applicability of numerical descriptors and statistical physics concepts to characterize complex biological systems observed at microscopic level through organ on chip approach. To end, employ data collected a microfluidic platform in which leukocytes can move suitably built channels toward their target. Leukocyte behavior is recorded by standard time lapse imaging. particular, analyze three groups human peripheral blood mononuclear cells (PBMC): heterozygous...
Scope of the present work is to infer migratory ability leukocytes by stochastic processes in order distinguish spontaneous organization immune cells against an insult (namely cancer). For this purpose, spleen from immunodeficient mice, selectively lacking transcription factor IRF-8 (IRF-8 knockout; KO), or immunocompetent animals (wild-type; WT), were allowed interact, alternatively, with murine B16.F10 melanoma ad hoc microfluidic environment developed on a LabOnChip technology. In...
Eosinophils are major effectors of Th2-related pathologies, frequently found infiltrating several human cancers. We recently showed that eosinophils play an essential role in anti-tumor responses mediated by immunotherapy with the ‘alarmin’ intereukin-33 (IL-33) melanoma mouse models. Here, we analyzed mechanisms which IL-33 mediates tumor infiltration and antitumor activities eosinophils. show recruits indirectly, via stimulation cell-derived chemokines, while it activates directly,...
The combination of chemotherapeutic agents with immune checkpoint inhibitors (ICIs) has revolutionized cancer treatment. However, its success is often limited by insufficient priming in certain tumors, including pediatric malignancies. In this report, we explore clinical trials currently investigating the use immunogenic cell death (ICD)-inducing chemotherapies ICIs for both adult and cancers. Given data available focused on recent preclinical studies evaluating efficacy these combinations...
Microfluidic impedance-based devices with coplanar electrode layout represent an attractive tool for low-cost, label-free, single-cell analysis. However, their usefulness has long been limited by the positional dependence of measured signals, i.e., identical particles traveling along different paths provide traces. In this paper we show that it is possible to significantly reduce unwanted effect via straightforward signal processing, exploiting richness information contained in recorded
While there is a great interest in microfluidic impedance cytometry as label-free approach for single-particle analysis, the accuracy of technique challenged by positional dependence issue, i.e. identical particles flowing microchannel along different trajectories provide signals. We solve this issue without resorting to particle focusing, means straightforward modification conventional wiring scheme standard chip comprising two pairs facing electrodes. Instead applying AC voltage electrodes...
Deformability is an essential feature of red blood cells (RBCs), enabling them to undergo significant shape change in response external forces. Impaired erythrocyte deformability associated with several pathologic conditions, and quantitative measurement RBC critical understanding diagnosing related diseases. Whereas traditional approaches cell mechanical characterization generally have limited throughput, emerging microscale technologies are opening new opportunities for high-throughput...
A full elucidation of events occurring inside the cancer microenvironment is fundamental for optimization more effective therapies. In present study, cross-talk between and immune cells was examined by employing mice deficient (KO) in interferon regulatory factor (IRF)-8, a transcription essential induction competent responses. The vivo results showed that IRF-8 KO were highly permissive to B16.F10 melanoma growth metastasis due failure their exert proper immunosurveillance. These found be...
A novel all-electrical approach for cross-sectional position detection of flowing particles and cells.
<italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Objective:</i> In aerobiological monitoring and agriculture there is a pressing need for accurate, label-free automated analysis of pollen grains, in order to reduce the cost, workload possible errors associated traditional approaches. xmlns:xlink="http://www.w3.org/1999/xlink">Methods:</i> We propose new multimodal approach that combines electrical sensing optical imaging classify grains...
Abstract Background Poor infiltration of functioning T cells renders tumors unresponsive to checkpoint-blocking immunotherapies. Here, we identified a combinatorial in situ immunomodulation strategy based on the administration selected immunogenic drugs and immunotherapy sensitize poorly T-cell-infiltrated neuroblastoma (NB) host antitumor immune response. Methods 975A2 9464D NB cell lines derived from spontaneous TH-MYCN transgenic mice were employed study drug combinations able enhancing...