A5053922930- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Lung Cancer Research Studies
- Cell death mechanisms and regulation
- Cancer therapeutics and mechanisms
- Ubiquitin and proteasome pathways
- Retinoids in leukemia and cellular processes
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- Protein Degradation and Inhibitors
- Angiogenesis and VEGF in Cancer
- Virus-based gene therapy research
- Biomarkers in Disease Mechanisms
- Neonatal Respiratory Health Research
- Neuroendocrine Tumor Research Advances
- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Signaling Pathways in Disease
- Lung Cancer Treatments and Mutations
Istituto Giannina Gaslini
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2011-2022
Boston Children's Hospital
1999-2012
Children's Hospital
2010
National Cancer Institute
2010
University of Bari Aldo Moro
2005-2010
A. O. Ordine Mauriziano di Torino
2008
University of Turin
2008
University of Alberta
2000-2007
ANT Foundation Italy Onlus
2006
Neuroblastoma, the most common solid tumor of infancy derived from sympathetic nervous system, continues to present a formidable clinical challenge. Sterically stabilized immunoliposomes (SIL) have been shown enhance selective localization entrapped drugs tumors, with improvements in therapeutic indices. We showed that SIL loaded doxorubicin (DXR) and targeted disialoganglioside receptor GD(2) [aGD(2)-SIL(DXR)] led inhibition metastatic growth experimental models human neuroblastoma. By...
It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if astrocyte processes, are to play crucial roles intercellular communication at synapses and neuron-astrocyte networks, could convey messages through vesicles. Our findings indicate, for first time freshly isolated processes prepared from adult rat cerebral cortex, can indeed participate system by releasing exosomes volume might target near or...
The prognosis of high-risk neuroblastoma (NB) remains poor, although immunotherapies with anti-GD2 antibodies have been reported to provide some benefit. Immunotherapies can be associated an IFNγ storm that induces in tumor cells the "adaptive immune resistance" characterized by de-novo expression Programmed Death Ligands (PD-Ls). Tumor also constitutively express PD-Ls response oncogenic signaling. Here, we analyze constitutive and inducible surface NB cells. We show virtually all HLA class...
Bortezomib is a selective and reversible inhibitor of the 26S proteasome that shows potent antitumor activity in vitro vivo against several human cancers adulthood. No data are available on bortezomib pediatric neuroblastoma.Ten neuroblastoma cell lines suspensions primary cells from three patients were tested for sensitivity to bortezomib. Colony formation, proliferation, cycle progression, apoptosis evaluated by clonogenic assay measuring 3H-thymidine incorporation, bromodeoxyuridine...
In the analysis of a neuroblastoma xenograft implanted in mice using two-dimensional maps, some 85 proteins were found to be up- or down-regulated (out total 264 detected by medium-sensitivity colloidal Coomassie stain). When these spots eluted and analysed mass spectrometry quadrupole time flight spectrometer, number envelopes different polypeptide chains. Out 74 identified, 52 (71%) singlets, 14 (19%) doublets, 6 (8%) triplets, 1 was quadruplet quintuplet. Analysis DeltapI DeltaMr all...
Abstract Cancer metabolism is characterized by an accelerated glycolytic rate facing reduced activity of oxidative phosphorylation. This “Warburg effect” represents a standard to diagnose and monitor tumor aggressiveness with 18 F-fluorodeoxyglucose whose uptake currently regarded as accurate index total glucose consumption. Studying cancer metabolic response respiratory chain inhibition metformin, we repeatedly observed reduction tracer marked increase in puzzling discordance brought us...
The present translational study aimed to verify whether serial 18F-FDG PET/CT predicts doxorubicin cardiotoxicity. Methods: Fifteen athymic mice were treated intravenously with saline (n = 5) or 5 7.5 mg of per kilogram each) and underwent dynamic small-animal PET beforehand afterward estimate left ventricular (LV) metabolic rate glucose (MRGlu). Thereafter, we retrospectively identified 69 patients who had been successfully a regimen doxorubicin, bleomycin, vinblastine, dacarbazine for...
Neuroblastoma (NB) represents the most frequent and aggressive form of extracranial solid tumor infants. Nucleolin (NCL) is a protein overexpressed partially localized on cell surface cells adult cancers. Little known about NCL pediatric tumors nothing reported NB.NB lines, Schwannian stroma-poor NB bone marrow (BM)-infiltrating were evaluated for expression by Flow Cytometry, Imaging Cytometry Immunohistochemistry analyses. The cytotoxic activity doxorubicin (DXR)-loaded nanocarriers...
BACKGROUND: Advanced-stage neuroblastoma resists conventional treatment; hence, novel therapeutic approaches are required. We evaluated the use of c-myb antisense oligodeoxynucleotides (asODNs) delivered to cells via targeted immunoliposomes inhibit c-Myb protein expression and cell proliferation in vitro.METHODS: Phosphorothioate asODNs control sequences were encapsulated cationic lipid, resulting particles coated with neutral lipids produce liposomes (CCLs). Monoclonal antibodies directed...
Abstract Purpose: In vivo evaluation of good manufacturing practice-grade targeted liposomal doxorubicin (TVT-DOX), bound to a CD13 isoform expressed on the vasculature solid tumors, in human tumor xenografts neuroblastoma, ovarian cancer, and lung cancer. Experimental Design: Mice were implanted with lung, ovarian, or neuroblastoma cells via pulmonary, peritoneal, orthotopic (adrenal gland) routes, respectively, treated, at different days post inoculation, multiple doses doxorubicin,...
Various NGR-containing peptides have been exploited for targeted delivery of drugs to CD13-positive tumor neovasculature. Recent studies shown that compounds containing this motif can rapidly deamidate and generate isoaspartate-glycine-arginine (isoDGR), a ligand alphavbeta3-integrin be also drug tumors. We investigated the role NGR isoDGR peptide scaffolds on their biochemical biological properties. Peptides cyclic CNGRC sequence could bind endothelial cells more efficiently than those...
Vinblastine and rapamycin displayed synergistic inhibition of human neuroblastoma-related angiogenesis. Here, we studied the antitumor activity vinblastine against neuroblastoma.Cell proliferation, cell cycle progression, apoptosis were evaluated by measuring (3)H-thymidine incorporation, bromodeoxyuridine uptake, phosphatidylserine exposure, respectively. The in vivo sensitivity neuroblastoma cells to was determined orthotopic neuroblastoma-engrafted mice. Angiogenesis assessed chick embryo...
Treatment of neuroblastoma is successful in less than half patients with high-risk disease. The antitumor activity a water soluble pegylated SN38 drug conjugate, EZN-2208, was compared CPT-11 (a prodrug for SN38) preclinical models human neuroblastoma.The vitro cytotoxicity EZN-2208 tested by counting trypan blue dye- and Annexin V-positive cells, whereas its therapeutic efficacy evaluated, terms survival, antiangiogenic activities, s.c. luciferase-transfected, pseudometastatic, orthotopic...
The Toll-like receptor 9 (TLR9) evolved to cope with pathogens, but it is expressed in a variety of tumors for reasons that are unclear. In this study, we report neuroblastoma (NB) cells express functional TLR9. Liposome-complexed CpG oligonucleotides inhibited the proliferation TLR9-expressing NB and induced caspase-dependent apoptotic cell death. Inhibitory (iODNs) abrogated these effects. RNA interference reduced TLR9 expression not level where responses were abolished. Compared free CpG,...
Sandra Cantilena 1,4 , Fabio Pastorino 3,4 Annalisa Pezzolo 3 Olesya Chayka 1 Vito Pistoia Mirco Ponzoni and Arturo Sala 1,2 Molecular Haematology Cancer Biology Unit, UCL Institute of Child Health, 30 Guilford st London WC1N 1EH UK 2 Brunel Genetics Pharmacogenomics, University, Uxbridge, Middlesex UB8 3PH, Laboratory Oncology, G. Gaslini Institute, Largo 5, 16147 Genova, Italy 4 Denotes equal contribution Received: December 30, 2011; Accepted: Published: 31, Keywords: HIF, hypoxia,...
Topoisomerase I inhibitors down-regulate HIF-1α leading to tumor growth inhibition, but only while maintaining sustained levels of drug exposure. EZN-2208, a multi-arm 40 kDa pegylated, releasable SN38-drug conjugate, provides higher, longer lasting exposure tumors SN38 in contrast that is released from CPT-11. EZN-2208 also consistently has greater antitumor activity than CPT-11 variety solid and hematological models. In this report, the ability PEG-SN38 its downstream targets, more potent,...
The anaplastic lymphoma kinase (ALK) is a tyrosine receptor that involved in the pathogenesis of different types human cancers, including neuroblastoma (NB). In NB, ALK overexpression, or point mutations, are associated with poor prognosis and advanced stage disease. Inhibition activity by small-molecule inhibitors lung cancers carrying translocations has shown therapeutic potential. However, secondary mutations may occur that, generate tumor resistance to inhibitors. To overcome we adopted...
Rearrangements involving the anaplastic lymphoma kinase (ALK) gene are defining events in several tumors, including large-cell (ALCL) and non-small cell lung carcinoma (NSCLC). In such cancers, oncogenic activity of ALK stimulates signaling pathways that induce transformation promote tumor growth. search for common activated by across different tumors types, we found hypoxia were significantly enriched ALK-rearranged ALCL NSCLC, as compared with other types T-cell or EGFR- K-RAS-mutated...