A5089430101- Medical Imaging and Pathology Studies
- Hematological disorders and diagnostics
- Radiomics and Machine Learning in Medical Imaging
- Extracellular vesicles in disease
- Sarcoidosis and Beryllium Toxicity Research
- Amyotrophic Lateral Sclerosis Research
- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Parkinson's Disease Mechanisms and Treatments
- melanin and skin pigmentation
- Advanced Electron Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Photosynthetic Processes and Mechanisms
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Retinal Development and Disorders
- Electron and X-Ray Spectroscopy Techniques
- HER2/EGFR in Cancer Research
- Migraine and Headache Studies
- Biochemical Analysis and Sensing Techniques
- Alzheimer's disease research and treatments
University of Genoa
2016-2025
Ospedale Policlinico San Martino
2023-2024
Creative Commons
2023
March of Dimes
2023
The University of Queensland
2008-2012
IFOM
2008-2010
Macroautophagy/autophagy, a defense mechanism against aberrant stresses, in neurons counteracts aggregate-prone misfolded protein toxicity. Autophagy induction might be beneficial neurodegenerative diseases (NDs). The natural compound trehalose promotes autophagy via TFEB (transcription factor EB), ameliorating disease phenotype multiple ND models, but its is still obscure. We demonstrated that regulates by inducing rapid and transient lysosomal enlargement membrane permeabilization (LMP)....
Although the importance of clathrin- and caveolin-independent endocytic pathways has recently emerged, key aspects these routes remain unknown. Using quantitative ultrastructural approaches, we show that clathrin-independent carriers (CLICs) account for approximately three times volume internalized by clathrin-mediated pathway, forming major pathway involved in uptake fluid bulk membrane fibroblasts. Electron tomographic analysis 3D morphology earliest shows they are multidomain organelles...
Abstract Background Although invasiveness is one of the major determinants poor glioblastoma (GBM) outcome, mechanisms GBM invasion are only partially understood. Among intrinsic and environmental processes promoting cell-to-cell interaction processes, eventually driving invasion, we focused on pro-invasive role played by Extracellular Vesicles (EVs), a heterogeneous group cell-released membranous structures containing various bioactive cargoes, which can be transferred from donor to...
SummaryClathrin-independent endocytosis is an umbrella term for a variety of endocytic pathways that internalize numerous cargoes independently the canonical coat protein Clathrin [1, 2]. Electron-microscopy studies have defined pleiomorphic CLathrin-Independent Carriers (CLICs) and GPI-Enriched Endocytic Compartments (GEECs) as related major players in such uptake [3, 4]. This CLIC/GEEC pathway relies upon cellular signaling activation through small G proteins, but mechanistic insight into...
The potential toxicity of engineered nanoparticles (NPs) for humans and the environment represents an emerging issue. Since aquatic ultimate sink NP deposition, development suitable assays is needed to evaluate impact NPs on biota. immune system a sensitive target NPs, conservation innate immunity useful basis studying common biological responses NPs. Suspension-feeding invertebrates, such as bivalves, are particularly at risk exposure, since they have extremely developed systems uptake nano...
Evidence indicates that endosomal entry promotes signaling by the Notch receptor, but mechanisms involved are not clear. In a search for factors regulate activation in endosomes, we isolated mutants Drosophila genes encode subunits of vacuolar ATPase (V-ATPase) proton pump. Cells lacking V-ATPase function display impaired acidification compartment and correlated failure to degrade endocytic cargoes. mutant cells internalize accumulate it lysosome, surprisingly also show substantial loss both...
It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if astrocyte processes, are to play crucial roles intercellular communication at synapses and neuron-astrocyte networks, could convey messages through vesicles. Our findings indicate, for first time freshly isolated processes prepared from adult rat cerebral cortex, can indeed participate system by releasing exosomes volume might target near or...
Triggering receptor expressed on myeloid cells 2 (Trem2) is a cell-specific gene in brain microglia, with variants that are associated neurodegenerative diseases, including Alzheimer's disease. Trem2 essential for microglia-mediated synaptic refinement, but whether contributes to shaping neuronal development remains unclear. Here, we demonstrate plays key role controlling the bioenergetic profile of pyramidal neurons during development. In absence Trem2, developing hippocampal cornus ammonis...
Collagen is involved in the formation of complex fibrillar networks, providing structural integrity tissues. Its low immunogenicity and mechanical properties make this molecule a biomaterial that extremely suitable for tissue engineering regenerative medicine (TERM) strategies human health issues. Here, first time, we performed thorough screening four different methods to obtain sponge collagenous suspensions (FSs) from C. reniformis demosponge, which were then chemically, physically,...
The treatment of non-small cell lung cancer (NSCLC) has changed dramatically with the advent immune checkpoint inhibitors (ICIs). Despite encouraging results, their efficacy remains limited to a subgroup patients. Circulating checkpoints in soluble (s) form and associated extracellular vesicles (EVs) represent promising markers, especially ICI-based therapeutic settings. We evaluated prognostic role PD-L1 two B7 family members (B7-H3, B7-H4), both EV-associated, cohort advanced NSCLC...
Abstract The secretome of mesenchymal stromal cells (MSCs) derived from different tissue sources is considered an innovative therapeutic tool for regenerative medicine. Although adipose tissue-and bone marrow-derived MSCs (ADSCs and BMSCs, respectively) share many biological features, the origins can be mirrored by variations in their secretory profile, particular secreted extracellular vesicles (EVs). In this study, we carried out a detailed comparative characterization middle- small-sized...
Glioblastoma (GBM), one of the deadliest brain tumors, accounts for approximately 50% all primary malignant CNS therefore novel, highly effective remedies are urgently needed. Boron neutron capture therapy, which has recently repositioned as a promising strategy to treat high-grade gliomas, requires conspicuous accumulation boron atoms in cancer cells. With aim selectively deliver sodium borocaptate (BSH, 12 B atoms-including molecule already employed clinics) GBM cells, we developed novel...
Extracellular vesicles (EVs) have emerged as potential vehicles for targeted drug delivery and diagnostic applications. However, achieving consistent reliable functionalization of EV membranes remains a challenge. Copper-catalyzed click chemistry, commonly used surface modification, poses limitations due to cytotoxicity interference with biological systems. To overcome these limitations, we developed standardized method functionalizing an membrane via copper-free chemistry. EVs derived from...
Ocular albinism type I (OA1) is an X-linked disorder characterized by severe reduction of visual acuity, strabismus, photophobia and nystagmus. Ophthalmologic examination reveals hypopigmentation the retina, foveal hypoplasia iris translucency. Microscopic both retinal pigment epithelium (RPE) skin melanocytes shows presence large granules called giant melanosomes or macromelanosomes. In this study, we have generated Oa1-deficient mice gene targeting (KO). The KO males are viable, fertile...
Abstract According to the “gain-of-toxicity mechanism”, neuronal loss during cerebral proteinopathies is caused by accumulation of aggregation-prone conformers misfolded cellular proteins, although it still debated which aggregation state actually corresponds neurotoxic entity. Autophagy, originally described as a variant programmed cell death, now emerging crucial mechanism for survival in response variety stressors, including nutrient deprivation, damage cytoplasmic organelles, or...
Non-alcoholic fatty liver disease (NAFLD) is a major cause of liver-related morbidity and mortality. Oxidative stress release pro-inflammatory cytokines, such as tumor necrosis factor α (TNFα), are consequences hepatic lipid overload, which can contribute to progression NAFLD non-alcoholic steatohepatitis (NASH). Also, mitochondria involved in the pathogenesis for their role metabolism. Definitive treatments NAFLD/NASH lacking so far. Silybin, extract milk thistle seeds, has previously shown...
Rationale: Mesenchymal stromal cells (MSCs)-derived extracellular vesicles (EVs) emerged as an innovative strategy for the treatment of chronic disorders such osteoarthritis (OA).Biological activity EVs is generally driven by their cargo, which might be influenced microenvironment.Therefore, pre-conditioning strategies, including modifications in culture conditions or oxygen tension could directly impact on MSCs paracrine activity.In this study we selected appropriate preconditioning system...
Inflammation is a physiological response to damaging stimulus but sometimes can be the cause of onset neurodegenerative diseases, atherosclerosis, and cancer. These pathologies are characterized by overexpression inflammatory markers like endothelial adhesion molecules, such as Vascular Cell Adhesion Molecule-1 (VCAM-1). In present work, development liposomes for therapeutic targeted delivery inflamed endothelia described. The idea exploit three-step pretargeting system based on...