A5079677559- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer Treatment and Pharmacology
- Ocular Oncology and Treatments
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Cancer Immunotherapy and Biomarkers
- Cancer therapeutics and mechanisms
- CAR-T cell therapy research
- Immune Response and Inflammation
- Immune cells in cancer
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Erythrocyte Function and Pathophysiology
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Cancer Research and Treatments
- T-cell and B-cell Immunology
- Lung Cancer Research Studies
- Synthesis and Biological Evaluation
- Extracellular vesicles in disease
- Cancer-related Molecular Pathways
- Cutaneous Melanoma Detection and Management
Ospedale Policlinico San Martino
2015-2024
Istituto Oncologico Veneto
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2023
University Hospital of Zurich
2023
University of Siena
2023
Weatherford College
2023
Alleanza Contro il Cancro
2004-2017
University of Genoa
2011-2015
Université Paris-Sud
2011
Université Paris Cité
2011
The treatment of non-small cell lung cancer (NSCLC) has changed dramatically with the advent immune checkpoint inhibitors (ICIs). Despite encouraging results, their efficacy remains limited to a subgroup patients. Circulating checkpoints in soluble (s) form and associated extracellular vesicles (EVs) represent promising markers, especially ICI-based therapeutic settings. We evaluated prognostic role PD-L1 two B7 family members (B7-H3, B7-H4), both EV-associated, cohort advanced NSCLC...
Many renal cancer patients experience disease recurrence after immunotherapy or combined treatments due to persistence of stem cells (CSCs). The identification reliable inducers CSC differentiation may facilitate the development efficient strategies for eliminating CSCs. We investigated whether interleukin 15 (IL-15), a regulator kidney homeostasis, induces CD105-positive (CD105+) CSCs from human cancers. CD105+ were cultured preserve their cell properties and treated with recombinant IL-15...
The combination of Programmed Cell Death 1 (PD-1) and Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4) blockade has dramatically improved the overall survival rate for malignant melanoma. Immune checkpoint blockers (ICBs) limit tumor's immune escape yet only approximately a third all tumors and, in most cases, limited amount time. Several approaches to overcome resistance ICBs are being investigated among which addition epigenetic drugs that expected act on both tumor cells. Guadecitabine,...
Although several genes have been associated to neuroblastoma (NB) predisposition and aggressiveness, further are likely involved in the overall risk of developing this pediatric cancer. We thus carried out whole-exome sequencing on germline DNA from two affected second cousins unlinked healthy relatives a large family with hereditary NB. Bioinformatics analysis revealed 6999 variations that were exclusively shared by familial NB cases. then considered for all unknown or rare missense...
Anti-PD-1 or anti-PD-L1 blocking monoclonal antibodies (mAbs) have shown potent anti-tumor effects in adult cancer patients and clinical studies recently been started pediatric cancers, including high-risk/relapsing neuroblastoma (NB). Therefore, we studied the of anti-PD-1/PD-L1 mAbs two syngeneic models disseminated NB generated by injection either Neuro2a NXS2 cells, which express PD-L1. In addition, tested combination these agents with immune-enhancing cytokine IL-21, Ecto-NTPDase...
Abstract: We studied the constitutive and interferon (IFN)‐γ‐induced expression of HLA class I antigen heavy chain, β 2 ‐microglobulin (β m), TAP‐1, TAP‐2 tapasin in a panel eleven neuroblastoma cell lines. Surface antigens was low eight out lines bearing MYC‐N amplification and/or 1p deletion, while two three lacking these genetic alterations showed normal expression. IFN‐γ treatment restored surface all Eight 11 did not express TAP‐1 mRNA them also lacked mRNA. m barely detectable or...
BackgroundNK cells have been long time considered as cytotoxic lymphocytes competent in killing virus-infected and tumors. However, NK may also play essential immuno-regulatory functions. In this context, the real existence of a defined subset with negative regulatory properties has hypothesized but never clearly demonstrated.Methodology/Principal FindingsHerein, we show vitro generation from human peripheral blood haematopoietic progenitors (PB-HP), novel non-cytolytic displaying mature...
Abstract Novel druggable targets have been discovered in neuroblastoma (NB), paving the way for more effective treatments. However, children with high-risk NB still show high mortality rates prompting a search of novel therapeutic options. Here, we aimed at repurposing FDA-approved drugs treatment by performing high-content screening 349 anticancer compounds library. In primary screening, employed three cell lines, grown as three-dimensional (3D) multicellular spheroids, which were treated...
Background: Uveal melanoma (UM), a rare cancer of the eye, is characterized by initiating mutations in genes G-protein subunit alpha Q (GNAQ), 11 (GNA11), cysteinyl leukotriene receptor 2 (CYSLTR2), and phospholipase C beta 4 (PLCB4) metastasis-promoting splicing factor 3B1 (SF3B1), serine arginine rich (SRSF2), BRCA1-associated protein 1 (BAP1). Here, we tested hypothesis that additional mutations, though occurring only few cases (“secondary drivers”), might influence tumor development....
Metastatic uveal melanoma (MUM) is a highly aggressive, therapy-resistant disease. Driver mutations in Gα-proteins GNAQ and GNA11 activate MAP-kinase YAP/TAZ pathways of oncogenic signalling. MEK-inhibitors do not significantly block MUM progression, likely due to persisting Statins inhibit activation by blocking the mevalonate pathway, geranyl-geranylation, subcellular localisation Rho-GTPase. We investigated drugs that affect valproic acid, verteporfin statins, combination with...
The ability of Interleukin-15 (IL-15) to activate many immune antitumor mechanisms renders the cytokine a good candidate for therapy solid tumors, particularly renal cell carcinoma. Although IL-15 is being currently used in clinical trials, function on kidney's components has not been extensively studied; we thus investigated role normal and tumor epithelial cells. Herein, analyzed expression biological functions proximal tubuli (RPTEC) their neoplastic counterparts, clear carcinomas (RCC)....
A bstract : Neuroblastoma (NB) is a solid tumor of infancy that presents high rate spontaneous regression, phenomenon likely reflects the activation an apoptotic/differentiation program. Indeed, level expression molecules involved in regulation apoptosis, such as p73 or survivin, prognostic factor NB patients. The caspase‐8 gene (CASP8) encodes key enzyme at top apoptotic cascade. Although methylation putative regulatory region CASP8 reportedly inhibits its transcription some MYCN ‐amplified...
Abstract Neuroblastoma is a stroma-poor (SP) aggressive pediatric cancer belonging to neuroblastic tumors, also including ganglioneuroblastoma and ganglioneuroma, two stroma-rich (SR) less tumors. Our previous gene-expression profiling analysis showed different CXCL13 mRNA expression between SP SR Therefore, we studied 13 tumors by reverse transcription quantitative real-time PCR (RT-qPCR) found that CXCR5b was more expressed in than predominantly Then, isolated Schwannian stromal cells...