A5017004109- Synthesis and biological activity
- Estrogen and related hormone effects
- Cancer therapeutics and mechanisms
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Bioactive Compounds and Antitumor Agents
- Synthesis and Biological Evaluation
- Metal complexes synthesis and properties
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Lysosomal Storage Disorders Research
- Click Chemistry and Applications
- Computational Drug Discovery Methods
- Synthetic Organic Chemistry Methods
- Quinazolinone synthesis and applications
- Receptor Mechanisms and Signaling
- Alzheimer's disease research and treatments
- Synthesis and Characterization of Heterocyclic Compounds
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Hemophilia Treatment and Research
- Alkaline Phosphatase Research Studies
- Protein Structure and Dynamics
- Sirtuins and Resveratrol in Medicine
- Cellular transport and secretion
- Toxin Mechanisms and Immunotoxins
Ospedale Policlinico San Martino
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2012-2018
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
2015-2018
Alleanza Contro il Cancro
2005-2017
Istituto Giannina Gaslini
2005-2016
University of Calabria
2015
Scuola Superiore Internazionale di Studi Universitari di Ricerca e Formazione
2015
University of Genoa
1998-2001
Tecnologie Avanzate (Italy)
1998-1999
Bisphenol A (BPA) is the principal constituent of baby bottles, reusable water metal cans, and plastic food containers. BPA exerts estrogen-like activity by interacting with classical estrogen receptors (ERα ERβ) through G protein-coupled receptor (GPR30/GPER). In this regard, recent studies have shown that GPER was involved in proliferative effects induced both normal tumor cells.We studied transduction signaling pathways which influences cell proliferation migration human breast cancer...
Biofilms, sedimented microbial communities embedded in a biopolymer matrix cause vast majority of human bacterial infections and many severe complications such as chronic inflammatory diseases cancer. Biofilms' resistance to the host immunity antibiotics makes this kind infection particularly intractable. Antimicrobial peptides (AMPs) are ubiquitous facet innate animals. However, AMPs activity was studied mainly on planktonic bacteria little is known about their effects biofilms. We...
Scope We have previously demonstrated that oleuropein ( OL ) and hydroxytyrosol HT reduce 17β‐estradiol‐mediated proliferation in MCF ‐7 breast cancer BC cells without affecting the classical genomic action of estrogen receptor ER ), but activating instead ERK 1/2 pathway. Here, we hypothesized this inhibition could be mediated by a G ‐protein‐coupled named GPER / GPR 30. Using ‐negative ‐positive SKBR 3 as experimental model, investigated effects on ‐mediated activation downstream pathways....
The multiple biological responses to estrogens are mainly mediated by the classical estrogen receptors ERα and ERβ, which act as ligand-activated transcription factors. exerts a main role in development of breast cancer; therefore, ER antagonist tamoxifen has been widely used although its effectiveness is limited de novo acquired resistance. Recently, GPR30/GPER, member seven-transmembrane G protein-coupled receptor family, implicated mediating effects various normal cancer cells. In...
Quercetin (Q1) is a flavonoid widely present in plants and endowed with several pharmacological properties mostly due to its antioxidant potential. Q1 shows anticancer activity could be useful cancer prevention. On the other hand, poorly soluble water unstable physiological systems, bioavailability very low.A small set of derivatives (Q2-Q9) has been synthesized following opportunely modified chemical procedures previously reported. Anticancer evaluated by MTT assay. Human Topoisomerases...
Herein, we report the synthesis and multiple anti-tumor properties of new gold silver carbene complexes. The chemical modifications, grounded on our previous studies, led us to identify a good lead complex, gold-based, whose biological features are very exciting promising in anti-cancer research could be further developed. Indeed, bis-[4,5-dichloro-(N-methyl-N'(2-hydroxy-2-phenyl)ethyl-imidazole-2-ylidene)gold(I)]+[dichloro-gold]- (AuL7) complex possesses ability interfere with at least...
Although the action of estrogens has been traditionally explained by binding to and transactivation nuclear estrogen receptor (ER)α ERβ, recently G protein-coupled GPR30/GPER involved in rapid signaling. We investigated ability two original molecules, which were named GPER-L1 GPERL2, bind activate GPER transduction pathway cancer cells. Competition assays, docking simulations, transfection experiments, real-time PCR, immunoblotting, gene silencing technology growth assays performed ascertain...
Mutational analysis of the IDUA gene was performed in a cohort 102 European patients with mucopolysaccharidosis type I. A total 54 distinct mutant alleles were identified, 34 which novel including 12 missense mutations, 2 nonsense splicing 5 micro-deletions, 1 micro-duplication translational initiation site mutation, and 'no-stop' change (p.X654RextX62). Evidence for pathological significance all mutations identified sought by means range methodological approaches, assessment evolutionary...
A meso-p-nitroaniline-calix[4]pyrrole derivative trans-coordinated to a Pt(II) center was synthesized and its structure solved by X-ray analysis. Adenosine monophosphate (AMP) used as model compound evaluate the potential for assisted delivery of metal DNA nucleobases via phosphate anion-binding properties calix[4]pyrrole unit. An NMR investigation kinetics AMP complexation in absence an H-bonding competing solvent (dry CD3CN) consistent with this hypothesis, but we could not detect...
Metal carbenic complexes have received considerable attention in both the catalysis and biological fields for their potential applications cancer antimicrobial therapies.A small series of new silver gold N-heterocyclic carbene has been designed synthesized. Among tested complexes, one compound was particularly active inhibiting anchorage-dependent -independent breast proliferation, inducing cell apoptosis via a mitochondria-related process. The antitumor activity associated to...
Alzheimer’s disease (AD) is a progressive and age-related neurodegenerative disorder affecting brain cells the most common form of “dementia”, because cognitive detriment which takes place. Neuronal disruption represents its major feature, due to cytosolic accumulation amyloid β-peptide (Aβ) leads senile plaques formation intracellular neurofibrillary tangles. Many studies have focused on design therapeutic use new molecules able inhibit Aβ aggregation. In this context, we evaluated ability...