A5049961945- Estrogen and related hormone effects
- Adrenal and Paraganglionic Tumors
- Hormonal Regulation and Hypertension
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Sperm and Testicular Function
- Cholesterol and Lipid Metabolism
- Glioma Diagnosis and Treatment
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sirtuins and Resveratrol in Medicine
- Growth Hormone and Insulin-like Growth Factors
- Hormonal and reproductive studies
- Reproductive Biology and Fertility
- Pituitary Gland Disorders and Treatments
- Reproductive Physiology in Livestock
- Prostate Cancer Treatment and Research
- Synthesis and biological activity
- Birth, Development, and Health
- Drug Transport and Resistance Mechanisms
- Phytoestrogen effects and research
- Edible Oils Quality and Analysis
- Reproductive System and Pregnancy
- Inflammatory mediators and NSAID effects
- Stress Responses and Cortisol
- Click Chemistry and Applications
University of Calabria
2015-2025
The University of Texas Southwestern Medical Center
2001-2019
To date, estrogen is the only known endogenous receptor (ER) ligand that promotes ER+ breast tumor growth. We report cholesterol metabolite 27-hydroxycholesterol (27HC) stimulates MCF-7 cell xenograft growth in mice. More importantly, cancer patients, 27HC content normal tissue increased compared to cancer-free controls, and further elevated. Increased correlated with diminished expression of CYP7B1, metabolizing enzyme, reduced CYP7B1 tumors associated poorer patient survival. Moreover,...
Abstract The growth of many breast tumors is stimulated by estradiol (E2), which activates a classic mechanism regulation gene expression and signal transduction pathways inducing cell proliferation. Polyphenols natural origin with chemical similarity to estrogen have been shown interfere tumor aim this study was investigate whether hydroxytyrosol (HT) oleuropein (OL), two polyphenols contained in extra‐virgin olive oil, can affect cancer proliferation interfering E2‐induced molecular...
Many studies have indicated that estrogens could a role in the regulation of testicular function. However, it remains uncertain whether are able to directly activate signaling pathways male germ cells. Estrogens synthesized by enzyme aromatase and classically act binding estrogen receptors (ERs)-α ERβ. Knockout mice for both receptor isoforms exhibit phenotype is less severe than knockout mice, suggesting existence an estrogen-binding may compensate lack ERs. Recently using...
In the current study we test hypothesis that liver receptor homologue-1 (LRH; designated NR5A2) is involved in regulation of steroid hormone production. The potential role LRH was assessed by first examining expression human steroidogenic tissues and second effects on transcription genes encoding enzymes steroidogenesis. closely related to factor 1 (SF1; NR5A1), which expressed most regulates several steroid-metabolizing enzymes. transcripts were at high levels ovary testis. Adrenal placenta...
Scope We have previously demonstrated that oleuropein ( OL ) and hydroxytyrosol HT reduce 17β‐estradiol‐mediated proliferation in MCF ‐7 breast cancer BC cells without affecting the classical genomic action of estrogen receptor ER ), but activating instead ERK 1/2 pathway. Here, we hypothesized this inhibition could be mediated by a G ‐protein‐coupled named GPER / GPR 30. Using ‐negative ‐positive SKBR 3 as experimental model, investigated effects on ‐mediated activation downstream pathways....
Aromatase converts testicular androgens to estrogens, which are essential for male fertility. expression in testis occurs via transcription from promoter II, and requires the presence of a nuclear receptor half-site that binds orphan steroidogenic factor-1 [SF-1 (nuclear 5A1)] mediate basal (in part) cAMP-induced transcription. We hypothesized liver homolog-1 (LRH-1) 5A2), closely related SF-1, could also play role regulating aromatase testis. demonstrate LRH-1 adult rat immature mouse...
Near term the human fetal adrenals (HFAs) initiate production of cortisol, which promotes organ maturation and acts to increase placental CRH biosynthesis. The objective present study was determine whether directly stimulates both cortisol expression steroidogenic enzymes in HFA-definitive zone cells. stimulated a time- dose-dependent manner, with an effective concentration as low 0.01 nm. In real-time RT-PCR experiments, treatment increased mRNA levels acute regulatory protein each needed...
Sexual hormones, estrogens and androgens, determine biological response in a tissue- gender-specific manner have pivotal role endocrine-mediated tumorigenesis. In situ estrogen production by aromatase is critical determinant for breast cancer growth progression. On the contrary, clinical vitro studies indicate that androgens protective mammary carcinogenesis. Here, we demonstrated, hormone-dependent cells, existence of functional interplay between androgen receptor (AR), orphan nuclear DAX-1...
The synthesis and the antiproliferative activity against human breast MCF-7, SkBr3 prostate LNCaP cancer cell lines of a series bis(indolyl)methane derivatives are reported. new compounds was first accomplished by reaction different indoles with trimethoxyacetophenone in presence catalytic amounts hydrochloric acid. A second procedure involving use oxalic acid dihydrate [(CO2H)2·2H2O] N-cetyl-N,N,N-trimethylammonium bromide water carried out led to better yields. Compound 5b significantly...
Abstract Context: Near term, the human fetal adrenal increases production of cortisol and dehydroepiandrosterone sulfate (DHEAS). DHEAS, which acts as substrate for placental estrogen production, induces key changes involved in parturition. Objective: The objective this study was to determine quantitatively effect CRH on mRNA levels enzymes needed DHEAS (steroidogenic acute regulatory protein, CYP11A, CYP17, SULT2A1), receptor (CRH-R) subtype(s) responsible action, CRH-R expression zone (FZ)...
Abstract The aim of this study was to investigate the role estrogens in Leydig cell tumor proliferation. We used R2C rat cells and testicular samples from Fischer rats with a developed tumor. Both experimental models express high levels aromatase estrogen receptor α (ERα). Treatment exogenous 17β-estradiol (E2) induced proliferation up-regulation cycle regulators cyclin D1 E, expression which blocked by addition antiestrogens. These observations led us hypothesize an E2/ERα–dependent...
Summary Spermatogenesis is a precisely controlled and timed process, comprising mitotic divisions of spermatogonia, meiotic spermatocytes, maturation differentiation haploid spermatids giving rise to spermatozoa. It well known that the maintenance spermatogenesis by gonadotrophins testosterone, effects which are modulated complex network locally produced factors, including oestrogens. However, it remains uncertain whether oestrogens able activate rapid signalling pathways directly in male...
Adrenocortical carcinoma (ACC) is a rare tumor with very poor prognosis and no effective treatment. ACC characterized by an increased production of IGF-II estrogen receptor (ER)-α up-regulation. The objective this study was to define the role played ERα in 17β-estradiol (E2)- IGF-II-dependent growth evaluate whether selective modulators are controlling vivo. human adrenocortical cell line H295R used as vitro model generate xenograft tumors athymic nude mice. In cells controlled expression...
Abstract We have previously shown that estrogens binding to estrogen receptor (ER) α increase proliferation of Leydig tumor cells. Estrogens can also bind G protein-coupled ER (GPER) and activation this either or decrease cell several types. The aim study was investigate GPER expression in R2C rat cells, evaluate effects its on define the molecular mechanisms triggered response activation. cells express activation, using specific ligand G-1, is associated with decreased initiation apoptosis....
We have previously demonstrated that estrogen receptor (ER) alpha (ESR1) increases proliferation of adrenocortical carcinoma (ACC) through both an estrogen-dependent and -independent (induced by IGF-II/IGF1R pathways) manner. Then, the use tamoxifen, a selective modulator (SERM), appears effective in reducing ACC growth vitro vivo. However, tamoxifen not only exerts antiestrogenic activity, but also acts as full agonist on G protein-coupled (GPER). Aim this study was to investigate effect...