A5076007276- Neuroblastoma Research and Treatments
- Reproductive Biology and Fertility
- Cancer, Hypoxia, and Metabolism
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Virus-based gene therapy research
- Neuroendocrine Tumor Research Advances
- Assisted Reproductive Technology and Twin Pregnancy
- Ovarian function and disorders
- Cancer therapeutics and mechanisms
- Renal and related cancers
- COVID-19 Impact on Reproduction
- Ubiquitin and proteasome pathways
- Prenatal Screening and Diagnostics
- Nanopore and Nanochannel Transport Studies
- Lung Cancer Research Studies
- Fuel Cells and Related Materials
- Ion-surface interactions and analysis
- Erythrocyte Function and Pathophysiology
- Cancer-related Molecular Pathways
- Genomic variations and chromosomal abnormalities
- Glutathione Transferases and Polymorphisms
- Epigenetics and DNA Methylation
- Protein Degradation and Inhibitors
- Cell death mechanisms and regulation
Ospedale Policlinico San Martino
2015-2025
National Cancer Research Institute
2006-2012
Alleanza Contro il Cancro
1994-2011
University of Genoa
2011
Schneider Children's Medical Center
2010
Institut Gustave Roussy
2010
University of Cologne
2010
Heidelberg University
2010
University Hospital Heidelberg
2010
Zero to Three
2008
STUDY QUESTIONIs the amount of cell-free DNA released by human embryos into culture medium correlated with embryo morphological features?
This study aims to evaluate levels of anxiety and depression in women, correlated with infertility per se treatments, highlighting predictors higher distress. Two validated standardized questionnaires, the Hospital Anxiety Depression Scale (HADS) Fertility Quality Life (FertiQoL), were administered 89 women both before their first cycle treatment again at end ovarian stimulation for vitro fertilization (IVF). Women's significantly than during itself. Stratifying three groups based on...
To evaluate the impact of a predefined gene expression-based classifier for clinical risk estimation and cytotoxic treatment decision making in neuroblastoma patients.Gene expression profiles 440 internationally collected specimens were investigated by microarray analysis, 125 which examined prospectively. Patients classified as either favorable or unfavorable 144-gene prediction analysis microarrays (PAM) established previously on separate set 77 patients. PAM classification results...
Neuroblastoma is the most common, pediatric, extra-cranial, malignant solid tumor. Despite multimodal therapeutic protocols, outcome for children with a high-risk clinical phenotype remains poor, long-term survival still less than 40%. Hereby, we evaluated potential of non-coding RNA expression to predict in high-risk, stage 4 neuroblastoma.We analyzed 481 Ultra Conserved Regions (UCRs) by reverse transcription-quantitative real-time PCR and 723 microRNAs microarrays 34 neuroblastoma...
In assisted reproduction technology, embryo competence is routinely evaluated on morphological criteria. Over the last decade, efforts in improving non-invasive assessment have looked into secretome of embryos. Human embryos release genomic DNA (gDNA) and mitochondrial (mtDNA) culture medium, mtDNA/gDNA ratio significantly correlated with fragmentation. Here, we investigate whether spent medium blastulation potential implantation. The was assessed 699 Day 3 media by quantitative polymerase...
Abstract Purpose: To optimize neuroblastoma treatment stratification, we aimed at developing a novel risk estimation system by integrating gene expression–based classification and established prognostic markers. Experimental Design: Gene expression profiles were generated from 709 specimens using customized 4 × 44 K microarrays. Classification models built 75 tumors with contrasting courses of disease. Validation was performed in an independent test set (n = 634) Kaplan–Meier estimates Cox...
PURPOSE To evaluate the prognostic role of MYCN oncogene amplification in children with neuroblastoma. PATIENTS AND METHODS Of 694 (age, 0 to 15 years) previously untreated neuroblastoma, 295 (42%) were evaluated at diagnosis for gene amplification. RESULTS Clinical characteristics and survival results patients studied 399 not comparable. In 48 (16%), was amplified (> or = three copies). Amplification more frequent older than 1 year, abdominal tumor (18% v 7%), advanced disease, normal...
About 50% of children with neuroblastoma (NB) show a metastatic disease and have poor prognosis. However, progression is greatly variable depends on patients' age MYCN oncogene amplification. To investigate the role in tumor aggressiveness, we performed array-CGH gene expression profiles three groups (G) NBs: G1, stage 4S patients single copy (MYCN-) tumors; G2, 4 patients, ≤ 18 months age, MYCN- tumors favorable outcome G3, Stage ≥ 19 unfavorable outcome. G1 was characterized by numerical...
Are there reasons that motivate young cancer survivors to ask for follow-up visits at an oncofertility unit?Cancer request the management of treatment-related side effects or ovarian reserve evaluation, even if not (or yet) wishing a pregnancy.Personalised counselling before gonadotoxic therapies is considered standard care women with newly diagnosed cancer. However, long-term these patients in unit described literature other than use cryopreserved material.We retrospectively examined rates...
Abstract BACKGROUND. Neuroblastic tumors (NTs) are largely comprised of neuroblastic (Nb) cells with various quantities Schwannian stromal (SS) cells. NTs show a variable genetic heterogeneity. NT gene expression profiles reported so far have not taken into account the cellular components. The authors genome‐wide analysis whole and microdissected Nb SS METHODS. analyzed 10 stroma‐poor (NTs‐SP) 9 stroma‐rich (NTs‐SR) by microarray technology. were isolated laser microdissection from NTs‐SP...
STUDY QUESTIONDoes storage time have any impact on the transcriptome of slowly frozen cryopreserved human metaphase II (MII) oocytes?
Individual response to oxidative stress, due exposure asbestos fibres plays a significant role in the malignant pleural mesothelioma (MPM) etiology. The differential impact on MPM risk of polymorphic alleles glutathione-S-transferases (GSTs) and manganese superoxide dismutase (MnSOD/SOD2) genes involved defence against damage has been investigated. Ninety cases 395 controls were genotyped using arrayed-primer extension technique. Logistic regression analysis was applied assess predictive...
The possible use of nanopores for single DNA molecules biosensing has been demonstrated, but much remains to do in order develop advanced engineered devices with enhanced stability, and controlled geometry surface properties. Here we present morphological electrical characterization solid state silicon nitride fabricated by focused ion beam direct milling chemically functionalized probe oligonucleotides, the final aim developing a versatile tool gene expression profiling.
Metastases in the bone marrow (BM) are grim prognostic factors patients with neuroblastoma (NB). In spite of extensive analysis primary tumor cells from high- and low-risk NB patients, a characterization freshly isolated BM-infiltrating metastatic is still lacking. Our aim was to identify proteins specifically expressed by cells, that may be relevant for therapeutic purposes. Sixty-six Italian children over 18 months age, diagnosed stage 4 NB, were included study. Metastatic patients' BM...
Reduction in motility and number of spermatozoa change their morphology are some the most relevant causes male infertility. Production reactive oxygen species may affect motility, DNA stability spermatozoa. This study aimed at evaluating effect combined treatment with myo-inositol, alpha-lipoic acid, folic betaine vitamins (namely, Sinopol®) on semen parameters sub-fertile men.We recruited 143 men, 26-53 years aged, no-smokers, without any testicular pathologies, a normal...
Abstract STUDY QUESTION Is there any association between the appearance of smooth endoplasmic reticulum aggregates (SERa) in oocytes and ovarian stimulation, embryological, clinical neonatal outcomes ICSI IVF cycles? SUMMARY ANSWER A suboptimal prolonged stimulation is detrimental to by inducing occurrence SERa, which reduces reproductive potential oocytes. WHAT IS KNOWN ALREADY Controlled recruits different qualities. Based on current evidence, it was agreed that non-homogeneous cytoplasm...
Some 30% to 80% of male sub-fertility may be associated with oxidative stress that damages spermatozoa and can decrease success in vitro fertilization (IVF) techniques. This multicenter, longitudinal, prospective study aimed investigate whether oral antioxidant supplementation improved the reproductive competence men who had low rates their previous intracytoplasmic sperm injection (ICSI) cycles without azoospermia or severe oligozoospermia any identifiable andrological disease....
The male reproductive system may be a potential target for SARS-CoV-2 since the presence of ACE and TMPRS2 receptors. After first report in semen COVID-19 patients, several papers reported that was not detected semen. However, some evidences indicated disease could impair parameters. During infection, or short period after, reduction sperm concentration motility an increase DNA fragmentation were observed, even asymptomatic patients. There is no conclusive data exploring whether this damage...