A5083711626- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Renal Diseases and Glomerulopathies
- Immune cells in cancer
- Advanced Proteomics Techniques and Applications
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Cancer, Hypoxia, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Cell Adhesion Molecules Research
- Chronic Kidney Disease and Diabetes
- RNA Interference and Gene Delivery
- Protein Interaction Studies and Fluorescence Analysis
- Cancer Immunotherapy and Biomarkers
- Glioma Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Diet and metabolism studies
Istituto Giannina Gaslini
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2016-2024
Creative Commons
2023
Ospedale Policlinico San Martino
2012-2023
National Institutes of Health
2020
Harvard University
2020
Rockefeller University
2020
Brigham and Women's Hospital
2020
Federation of American Societies for Experimental Biology
2020
Yale University
2020
Natural killer (NK) cells play a key role in tumor immune surveillance. However, adoptive immunotherapy protocols using NK have shown limited clinical efficacy to date, possibly due escape mechanisms that inhibit cell function. In this study, we analyzed the effect of coculturing melanoma and on their phenotype We found inhibited expression major receptors trigger function, including NKp30, NKp44, NKG2D, with consequent impairment cell-mediated cytolytic activity against various lines. This...
Neutrophil extracellular traps (NET) formation is part of the neutrophil response to infections, but excessive or inappropriate NETosis may trigger production autoantibodies and cause organ damage in autoimmune disorders. Spontaneously netting neutrophils are not frequent induction NET vitro by selected stimuli necessary investigate their structure. In present work, protein composition post-translational modifications produced under different have been studied means proteomic analysis....
Glomerular targets of autoimmunity in human membranous nephropathy are poorly understood. Here, we used a combined proteomic approach to identify specific antibodies against podocyte proteins both serum and glomeruli patients with (MN). We detected anti-aldose reductase (AR) anti-manganese superoxide dismutase (SOD2) IgG(4) sera MN. also eluted high titers anti-AR anti-SOD2 from microdissected three biopsies MN kidneys but not other glomerulonephritides characterized by IgG deposition (five...
Tumor chemoresistance is associated with high aerobic glycolysis rates and reduced oxidative phosphorylation, a phenomenon called "Warburg effect" whose reversal could impair the ability of wide range cancer cells to survive in presence or absence chemotherapy. In previous studies, Short-term-starvation (STS) was shown protect normal organs but sensitize different cell types chemotherapy mechanisms responsible for these effects are poorly understood. We tested cytotoxicity Oxaliplatin (OXP)...
Neuroblastoma (NB) is the most common extracranial solid tumor in childhood, with grim prognosis a half of patients. Exosomes are nanometer-sized membrane vesicles derived from multivesicular bodies (MVBs) endocytic pathway and released by normal neoplastic cells. Tumor-derived exosomes have been shown different model systems to carry molecules that promote cancer growth dissemination. In this respect, we here performed first characterization proteomic analysis isolated human NB cell lines...
Chimeric antigen receptor (CAR) T-cell therapy has been shown to be dramatically effective in the treatment of B-cell malignancies. However, there are still substantial obstacles overcome, before similar responses can achieved patients with solid tumors. We evaluated both vitro and a preclinical murine model efficacy different 2nd 3rd generation CAR constructs targeting GD2, disial-ganglioside expressed on surface neuroblastoma (NB) tumor cells. In order address potential safety concerns...
// Grazia Carbotti 1 , Gaia Barisione Irma Airoldi 2 Delia Mezzanzanica 3 Marina Bagnoli Simone Ferrero 4 Andrea Petretto 5 Fabbi and Silvano Ferrini Department of Integrated Oncological Therapies, IRCCS AOU San Martino-IST Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy Laboratory Oncology, Giannina Gaslini, Experimental Oncology Molecular Medicine, Fondazione dei Tumori, Milan, Surgery, Unit Obstetrics Gynaecology, DINOGMI, University Genoa Core Facilities, Correspondence to:...
Microglia are brain-resident immune cells and regulate mechanisms essential for cognitive functions. Down syndrome (DS), the most frequent cause of genetic intellectual disability, is caused by a supernumerary chromosome 21, containing also genes related to system. In hippocampus Dp(16) mouse model DS individuals, we found activated microglia, as assessed their morphology; activation markers; and, mice, electrophysiological profile. Accordingly, increased pro-inflammatory cytokine levels...
Neutrophil extracellular traps (NET) expose modified antigens for autoantibodies in vasculitis. Little is known about levels and removal pathways of NET systemic lupus erythematosus (SLE), especially nephritis (LN). We determined circulating defined large subsets patients with incident SLE (iSLE), some whom had new-onset nephritis.Serum (ELISA), DNase1/DNase1L3 DNase activity (functional assay) were 216 iSLE [103 LN (iLN)], 50 other primary glomerulonephritis, healthy controls. Ex vivo...
Glioblastoma multiforme (GBM), also known as grade IV astrocytoma, represents the most aggressive primary brain tumor. The complex genetic heterogeneity, acquired drug resistance, and presence of blood-brain barrier (BBB) limit efficacy current therapies, with effectiveness demonstrated only in a small subset patients. To overcome these issues, here we propose an anticancer approach based on ultrasound-responsive drug-loaded organic piezoelectric nanoparticles. This nanoplatform consists...
A dysfunctional gut microbiota-brain axis is emerging as a potential pathogenic mechanism in epilepsy, particularly pediatric forms of epilepsy. To add new insights into gut-related changes acquired epilepsy that develops early life, we used multi-omics approach rat model with 56% incidence The presence spontaneous seizures was assessed adult rats (n = 46) 5 months after status epilepticus induced by intra-amygdala kainate at postnatal day 13, 2 weeks (24/7) ECoG monitoring. Twenty-six...
Glioblastoma multiforme (GBM) is the most aggressive brain cancer, characterized by a rapid and drug-resistant progression. GBM "builds" around its primary core genetically heterogeneous tumor-microenvironment (TME), recruiting surrounding healthy cells releasing various intercellular signals. Glioma-associated microglia (GAM) represent largest population of collaborating cells, which, in TME, usually exhibit anti-inflammatory M2 phenotype, thus promoting an immunosuppressing environment...
Polydopamine nanoparticles (PDA NPs) are proposed as an anti-cancer tool against hepatocellular carcinoma through the combination of near-infrared (NIR)-mediated hyperthermia and loading with a chemotherapeutic drug, sorafenib (SRF). Cell membranes isolated from liver cancer cell line (HepG2) have been exploited for coating (thus obtaining CM-SRF-PDA NPs), to promote homotypic targeting toward cells. The selective ability combined photothermal activity NPs following NIR irradiation evaluated...
Even if nephrotic syndrome is characterized by massive urinary loss of major plasma proteins, a clear structural characterization based on proteomics has never been reported. Urine and 23 patients with different idiopathic syndromes (10 steroid-sensitive minimal-change nephropathy, seven steroid-resistant FSGS, six membranous glomerulonephritis) were analyzed two-dimensional electrophoresis in soft gel, Western blot, matrix-assisted laser desorption/ionization time flight mass spectrometry;...
Tumor cell plasticity is a major obstacle for the cure of malignancies as it makes tumor cells highly adaptable to microenvironmental changes, enables their phenotype switching among different forms, and favors generation prometastatic subsets. Phenotype toward more aggressive forms involves functional, phenotypic, morphologic which are often related process known epithelial-mesenchymal transition (EMT). In this study, we report natural killer (NK) may increase malignancy melanoma by...
Abstract NETs constitute a network of DNA and proteins released by neutrophils in response to infectious immunologic triggers. NET are recognized as autoantigens ANCA vasculitis; limited knowledge is available other autoimmune pathologies. The composition produced ex vivo resting Phorbol-myristate acetate (PMA) stimulated was analyzed high-throughput Fusion Orbitrap technology 16 patients with Systemic Lupus Erythematosus/Lupus nephritis (9 SLE/7 LN) 11 controls. Seven-hundred were...
The proposed nanoplatform represents an efficient tool for a synergic treatment based on hyperthermia and chemotherapy against glioblastoma.
The release of soluble ligands activating Natural Killer (NK) cell receptors may represent a regulatory mechanism NK function both in physiologic and pathologic conditions. Here, we identified the extracellular matrix protein Nidogen-1 (NID1) as ligand NKp44, an important receptor expressed by activated cells. When released molecule, NID1 regulates modulating NKp44-induced IFN-γ production or cytotoxicity. In particular, it also modulates induced Platelet-Derived Growth Factor (PDGF)-DD...
Prostate malignancy represents the second leading cause of cancer-specific death among male population worldwide. Herein, enhanced intracellular magnetic fluid hyperthermia is applied in vitro to treat prostate cancer (PCa) cells with minimum invasiveness and toxicity highly specific targeting. We designed optimized novel shape-anisotropic core-shell-shell nanoparticles (i.e., trimagnetic - TMNPs) significant magnetothermal conversion following an exchange coupling effect external...
Background Epithelial to mesenchymal transition (EMT) endows cancer cells with pro-metastatic properties, which appear most effective when enter an intermediate hybrid (H) state, characterized by integrated (M) and epithelial (E) traits. The reasons for this advantage are poorly known and, especially, it is totally unexplored whether the interplay between H-cells NK could have a role. Here we characterize mechanics of non-small cell lung (NSCLC) their subset cancer-initiating (CICs),...
Non-alcoholic fatty liver disease (NAFLD), or hepatic steatosis, has become one of the most common pathologies among liver-related disorders. The rapid accumulation fat in this organ, and consequent increment oxidative stress levels, have been recognized as main factors involved pathological mechanism underlying progressive non-alcoholic steatohepatitis. Experimental epidemiological data shown how antioxidant compounds rich phenolic groups, such resveratrol quercetin, are particular...
The basic mechanism for idiopathic FSGS still is obscure. Indirect evidence in humans and generation of by oxidants experimental models suggest a role free radicals. In vitro studies demonstrate main plasma albumin as antioxidant, its modification representing chemical marker oxidative stress. With the use complementary liquid chromatography electron spray ionization tandem mass spectrometry (LC-ESI-MS/MS) biochemical methods, was characterized 34 patients with FSGS; 18 had received renal...